Study of Toxic Profile of Staphylococcus Aureus Isolated From Raw Milk Samples of Mastitis SOR Consumption in Oran Area, Algeria

Authors

  • Nadjia BENHAMED  Department of Biotechnology, University of USTO-MB, Oran, Algeria
  • Fatma BELHOUCINE  Department of Biotechnology, University of USTO-MB, Oran, Algeria
  • Mustapha MOHAMED BENKADA  Department of Biotechnology, University of USTO-MB, Oran, Algeria
  • Philippe GAUTIER  EA 125Microbiologie-Risques Infectieux, University of Rennes 1, Rennes, France
  • Amel ALIOUA  Department of Biotechnology, University of USTO-MB, Oran, Algeria
  • P-Y DONNIO  EA 125Microbiologie-Risques Infectieux, University of Rennes 1, Rennes, France
  • Mebrouk KIHAL  Laboratory of Applied Microbiology, University of Oran 1 Ahmed Benbela, Oran, Algeria

Keywords:

milk, mastitis, Staphylococcus aureus, Toxic profile, pvl, TSST-1, sea, seb.

Abstract

Mastitis S. aureus is considered one of the major diseases in dairy cattle. The presence of S. aureus causes damage to the mammary gland tissue that will impact milk production in both volume and quality. The many virulence factors such as toxins and proteins or surface polysaccharides are involved in the pathology. This study determines the toxic profile of S. aureus strains isolated from raw milk samples from bovine mastitis diagnosed in dairy farms in Oran.

After identification by MALDI-TOF, spa typing and MLST have identified the phylogenetic position of the strains. Search for virulence factors is performed by real-time PCR TaqMan type, to detect manipulation in one of the four genes of virulence factors of S. aureus: tst for TSST-1, pvl leucocidin for ED, sea for enterotoxin A, seb for enterotoxin B, in this study sought.

Toxins research shows that only a few strains have proven carriers of different virulence genes including the gene encoding the pvl Panton-Valentine leukocidin. Other strains were positive for the presence of tst gene encoding the toxin of toxic shock syndrome (TSST-1), 100% of the Staphylococcus aureus isolates identified were sensitive to methicillin.

In conclusion, the results obtained in this work have determined the phylogenetic profile, toxic and sensitivity profile of the méticiline strains studied. Stem found produisantes as the Panton-Valentine leukocidin are sensitive to méticilline. Cette study is the first molecular characterization study of animal strains of S. aureus isolated in the region of Oran.

References

  1. Akineden, O., Annemuller, C. and Hassan, A.A, Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. 2001.Clin. Diagn Lab. Immunol. 8:959–964.
  2. Velusamy, S, Ashish, A, Sawant, A, Barbara, E, Gillespie, S, Headrick, J, Lorenza, C. and Stephen, P., Prevalence of Enterotoxin and Toxic Shoc Sydrome Toxin Genes in S. aureus isolated from milk of cow with Mastitis,food. 2006. Path. And disease 3:274-283.
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International Journal of Scientific Research in Science and Technology

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Published

2017-06-30

Issue

Volume 1, Issue 6, CIBA - Conference Proceeding-2015

Section

Research Articles

License

Copyright (c) IJSRST

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Nadjia BENHAMED, Fatma BELHOUCINE, Mustapha MOHAMED BENKADA, Philippe GAUTIER, Amel ALIOUA, P-Y DONNIO, Mebrouk KIHAL, " Study of Toxic Profile of Staphylococcus Aureus Isolated From Raw Milk Samples of Mastitis SOR Consumption in Oran Area, Algeria, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 1, Issue 6, pp.4-6, CIBA - Conference Proceeding-2015.