Biological Control of Botrytis cinerea by Bacillus sp. Strain S7LiBe Under Abiotic Stress

Authors

  • Bensidhoum Leila  Department of Microbiology, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria
  • Rai Abdelwahab  Department of Microbiology, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria
  • Tabli Nassira  Department of Microbiology, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria
  • Kahouadji Nabila  Department of Microbiology, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria
  • Khaber Meriem   Department of Microbiology, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria
  • Nabti Elhafid  Department of Microbiology, University of Bejaia, Targa Ouzemmour, Bejaia, Algeria

Keywords:

Bacillus sp., Botrytis cinerea, PGPR, Biocontrol.

Abstract

In this investigation, the isolate S7LiBe isolated from trefoil rhizosphere in Barbacha, Bejaia (northern Algeria), was selected on the basis of plant growth promoting attributes. This isolate identified as Bacillus sp. based on 16SrDNA sequence analysis, exhibits the highest similarity of 99.1% with Bacillus pichinotyi AF519463 andAF519460 and Bacillus sp. JX152775. Bacillus sp. S7LiBe has been subjected to the study of its antifungal activity against Botrytis cinerea in vitro and in vivo on the detached lettuce leaves. The effect of some abiotic factors on the performance of this bacterial strain as biocontrol agent and the production of some antifungal metabolites (siderophores, ammonia, hydrogen cyanide and chitinase) were also carried out. The results showed that Bacillus sp. S7LiBe strain inhibit the mycelial growth in high level (70%±1,33) and the fungal spores germination (82,54%±3,58 ). The strain produce siderophores, ammonia, hydrogen cyanide and chitinase. Its antagonistic activity was found to be optimal on CZA modified medium at a pH of 6.5 and a temperature of 30 ° C. The antagonist activity on the detached lettuce leaves showed that the bacterial strain inhibit 57.0% of the lesion extension.

References

  1. S. Compant,  B. Duffy, J. Nowak, C. Clement, E. A. Barka, 2005, Use of plant growth promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl. Environ. Microbiol., 71 4951-4959.
  2. R. Borris, Use of Plant-Associated Bacillus Strains as Biofertilizers and Biocontrol Agents in Agriculture, in Bacteria in agrobiology: Plant growth responses, Maheshwari DK. (Ed.), Springer, Berlin, Heidelberg 2011.
  3. W. E. McKeen, 1974, Mode of penetration of epidermal cell walls of vicia faba by botrytis cinerea. Phytopathology, 64(4) 461.
  4.  M. K. Hausbeck and S. P. Penny-Packer, 1991, InXuence of grower activity on concentration of airborne conidia of Botytis cinerea among geranium cuttings, Plant disease, 75 1236-1243.
  5. G. Peng, J. C. Sutton, D. W. Li, 1996, Sites of infection in tomato stems by Botrytis cinerea biological control of Botrytis cinerea on tomato plant by use of epiphytic yeasts Candida guilliermondii strain 101 and US 7 and Candida oleophila strain I-182: I. in vivo study Can J. Plant Pathol Biol. Control, 18 143-150.
  6. T. M. O´Neil, D. Shtienberg, Y. Elad, 1997, Effect of some Host and microclimate factors on infection of tomato stems by botrytis cinerea, Plant disease, 81(1) 36-40.
  7. Akhtar MS et Siddiqui ZA. (2010). Role of Plant Growth Promoting Rhizobacteria in Biocontrol of Plant Diseases and Sustainable Agriculture, in Plant Growth and Health Promoting Bacteria, Maheshwari DK. (Ed.), Springer-Verlag Berlin Heidelberg 2010.
  8. N. Kokalis-Burelle, E. N. Vavrina, E. N. Rosskopf, R. A. Shelby. 2002. Field evaluation of plant growth-promoting rhizobacteria amended transplant mixes and soil solarization for tomato and pepper production in Florida. Plant Soil,  238 257-266.
  9. Y. Bai, X. Zhou, D. L. Smith. 2003. Enhanced soybean plant growth resulting from coinoculation of Bacillus strains with Bradyrhizobium japonicum. Crop Sci 43:1774-1781
  10. J. W. Kloepper, C. M. Ryu, S. Zhang, 2004, Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94 1259-1266.
  11. C. K. Deepa, S. G. Dastager, A. Pandey. 2010. Isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (Vigna unguiculata (L.) Walp.) seedling growth. World J Microbiol Biotechnol. 26(7) 1233-1240.
  12.  Y. Elad and A. Stewart. Microbial control of Botrytis spp., in Y. Elad, B. Williamsom and P. Tudzynski (Eds.), Botrytis: Biology, Pathology and Control. Springer, Netherlands, 2007.
  13.  J. Raaijmakers, M. Vlami and T. Jorge. 2002. Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek. 81 537-547.
  14.  M. do Vale Barreto Figueiredo, L. Seldin, F. F. de Araujo and R. de Lima Ramos Mariano. Plant Growth Promoting Rhizobacteria: Fundamentals and Applications, in Plant Growth and Health Promoting Bacteria, Maheshwari DK. (Ed.), Springer-Verlag Berlin Heidelberg 2010
  15. O. Susana Correa and M. Abel Soria. Potential of Bacilli for Biocontrol and Its Exploitation in Sustainable Agriculture, in Plant Growth and Health Promoting Bacteria, Maheshwari DK. (Ed.), Springer-Verlag Berlin Heidelberg 2010
  16. B. Bouizgarne. Bacteria for Plant Growth Promotion and Disease Management, in Bacteria in Agrobiology: Disease Management, Maheshwari DK. (Ed.), Springer-Verlag Berlin Heidelberg 2013.
  17. V. Govindasamy, M. Senthilkumar, V. Magheshwaran, U. Kumar, P. Bose, V. Sharma and K. Annapurna. Bacillus and Paenibacillus spp.: Potential PGPR for Sustainable Agriculture, in Plant Growth and Health Promoting Bacteria, Maheshwari DK. (Ed.), Springer-Verlag Berlin Heidelberg 2010.
  18. W. Ludwig, O. Strunk, R. Westram, L. Richter, H. Meier, Yadhukumar, A. Buchner, T. Lai, S. Steppi, G. Jobb, W. Förster, I. Brettske, S. Gerber, A.W. Ginhart, O. Gross, S. Grumann, S. Hermann, R. Jost, A. König, T. Liss, R. Lüßmann, M. May, B. Nonhoff, B. Reichel, R. Strehlow, A. Stamatakis, N. Stuckmann, A. Vilbig, M. Lenke, T. Ludwig, A. Bode, K.H. Schleifer. 2004. ARB: a software environment for sequence data. Nucl. Acids. Res. 32 1363-1371.
  19. G.J. Olsen, H. Matsuda, R. Hagstrom, R. Overbeek. 1994. Fast DNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood, Computer Appl. Biosci. 10 41-48.
  20. I. P. Sagahón, M. A. Anducho-Reyes, H. V. Silva-Rojas, A. Arana-Cuenca, A. Alejandro Tellez-Jurado, I. O. Cárdenas-Álvarez and Y. Mercado-Flores. 2011. Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora. International Journal of Molecular Sciences. 12 5522-5537.
  21. Z. N. Sadfi, I. Hannachi, D. Andurand, B. Essghaier, A. Boudabous and P. Nicot. 2008. Biological control of Botrytis cinerea on stem wounds with moderately halophilic bacteria. World J Microbiol Biotechnol. 24 2871-2877.
  22. H. Lorck. 1948. Production of hydrocyanic acid by bacteria. Physiol. Plant. 1 142-146.
  23. J. C. Cappucino and N. Sherman. 1992. Negative staining. Microbiology: A laboratory Manual. 3 125-179.
  24. B. Schwyn, J. B. Neilands. 1986. Universal chemical assay for the detection and determination of siderophores, Anal. Biochem. 160 47-56.
  25. J. Kopečný, B. Hodrová and C. S. Stewart. 1996. The isolation and characterization of a rumen chitinolytic bacterium. Lett. Appl. Microbiol. 23 195-198.
  26. P. Marschner and S. Timonen. 2006. Bacterial community composition and activity in rhizosphere of roots colonized by arbuscular mycorrhizal fungi, in K. G. Mukerji, C. Manoharachary, J. Singh (Eds.), Microbiam activity in the rhizosphere, Soil Biology.7 139-154.
  27. F. Jamali, A. Sharifi-Tehrani, M.P. Lutz and M. Maurhofer. 2009. Influence of Host Plant Génotype, Presence of a pathogen, and Coinoculation with Pseudomonas fluorescens Strains on the rhizosphere expression of hydrogen Cyanide- and 2,4-Diacetylphloroglucinol biosynthesis Genes in P. fluorescens biocontrol Strain CHAO. Microb Ecol. 57 267-275.
  28. P. Calvo, E. Ormeño-Orrillo, E. Martínez-Romero and D. Zúñiga. 2010. Characterization of Bacillus isolates of potato rhizosphere from andean soils of peru and their potential PGPR characteristics. Brazilian Journal of Microbiology. 41 899-906.
  29. C. Alabouvette, C. Olivain and C. Steinberg. 2006. Biological control of plant diseases. The European situation. European Journal of Plant Pathology. 114 329-341.
  30. R. Guetsky, Y. Elad, D. Shtienberg et A. Dinoor. 2002. Establishment, survival and activity of the biocontrol agents Pichia guillermondii and Bacillus mycoides applied as a mixture on strawberry plants. Biocontrol Science and Technology. 12 705-714.
  31. S. Alstrom. 2001. Characteristics of bacteria from oil seed rape in relation to their biocontrol of activity against Verticillium dahliae. J Phytopathol. 149 57-64.
  32. R. E. Wheatley. 2002. The consequences of volatile organic compound mediated bacterial and fungal interactions. Antonie Van Leeuwenhoek. 81 357-364.
  33. M. H. Charest, C. J. Beauchamp and H. Antoun. 2005. Effects of the humic substances of de-inking paper sludge on the antagonism between two compost bacteria and Pythium ultimum. FEMS Microbiology Ecology. 52 219-227.
  34. O.H. Ahmed, H. Aminuddin and M. H. A. Husni. 2008. Ammonia volatilization and ammonium accumulation from urea mixed with zeolite and triple superphosphate. Acta Agric. Scand. 58 182-186.
  35. P. A. Wani, M. S. Khan and A. Zaidi. 2007. Synergistic effects of the inoculation with nitrogen fixing and phosphate-solubilizing rhizobacteria on the performance of field grown chickpea. J. Plant Nutr. Soil Sci. 170 283-287.
  36. C. M. Ryu, M. A. Farag, C. H. Hu, M. S. Reddy, H. X. Wei, P. W. Paré and J. W. Kloepper. 2003. Bacterial volatiles promote growth in Arabidopsis. Proc. Natl. Acad. Sci.100 4927-4932.
  37. S. Wang, T. Hu, Y. Jiao, J. Wie, K. Cao. 2009. Isolation and characterization of Bacillus subtilis EB-28, an endophytic bacterium strain displaying biocontrol activity against Botrytis cinerea Pers. Front. Agric. China. 3 247-252.
  38. P. Prashar, N. Kapoor, and S. Sachdeva. 2013. Isolation and Characterization of Bacillus sp with In-vitro Antagonistic Activity against Fusarium oxysporum from Rhizosphere of Tomato.15 1501-1512.
  39. T. Kavitha, R. Nelson and J. S. Josephin. 2013. Screening of rhizobacteria for plant growth promoting traits and antifungal activity against charcoal rot pathogen Macrophomina phaseolina. Int J Pharm Bio Sci. 4 177 - 186.
  40. E. H. Nabti, N. Mokrane, M. Ghoul, H. Manyani, M. Dary and M. G. Megias. 2013. Isolation and characterization of two halophilic Bacillus (B. licheniformis and Bacillus sp) with antifungal activity. Journal of Ecology of Health & Environment. 1 13-17.
  41. S. H. Ji , M. A. Gururani and C. S. Chul. 2014. Isolation and characterization of plant growth promoting entophytic diazotrophic bacteria from Korean rice cultivars. Microbiological Research. 169 83- 98.
  42. R. Baker, Y. Flad, B. Sneh. 1986. Physical, biological and host factors in iron competition in soils in T. R. Swinburne (ed) Iron siderophores and plant diseases. Plenum press, New York.
  43. J. E. Loper and M. D. Henkels. 1999. Utilization of heterologous siderophores enhances levels of iron available to Pseudomonas putida in the rhizosphere. Appl. Environ. Microbiol. 65 5357-5363.
  44.  D. J. O’Sullivan and F. O’Gara. 1992. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiol. Rev. 56 662-676.
  45. B. K. Duffy, G. Défago. 1999. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Applied and Environmental Microbiology. 65 2429-38.
  46. W. De Boer, P. J. A.K. Gunnewiek, P. Lafeber, J. D. Janse, B. E. Spit and J. W. Woldendorp. 1998. Antifungal properties of chitinolytic dune soil bacteria. Soil Biol Biochem. 30 193-203.
  47. S. L. Wang, W. J. Hsiao and W. T. Chang. 2002. Purification and characterization of an antimicrobial chitinase extracellular produced by Monascus purpureus CCRC31499 in a shrimp and crab shell powder medium. J Agr Food Chem. 50 2249-55.
  48. L. Chernin, Z. Ismailov, S. Haran, and I. Chet. 1995. Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Appl. Environ. Microbiol. 61 1720-1726.
  49. D. Y. Kobayashi, R. M. Reedy J. A. Bick and P. V. Oudemans. 2002. Characterization of a chitinase gene from Stenotrophomonas maltophilia strain 34S1 and its involvement in biological control. Appl. Environ. Microbiol. 68 1047-1054.
  50. S. Freeman, O. Minzm, I. Kolesnik, O. Barbul, A. Zveibil, M. Maymon, Y. Nitzani, B. Kirshner, D. Rav-David, A. Bilu, A. Dag, S. Shafir and Y. Elad. 2004. Trichoderma biocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry. Eur. J. Plant Pathol. 110 361-370.
  51. I. O. M. El-Hamshary, S. E. M. Abo-Aba, N. Awad and A. M. Gomaa. 2008. Genetic Profile of Bacillus cereus and Bacillus subtilis indigenous isolates and their performance as bio-control agent against the plant pathogen Fusarium oxysporum. Research Journal of Cell and Molecular Biology. 2 30-38.
  52. L. L. Burpee. 1990. The influence of abiotic factors on biological control of soilborne plant pathogenic fungi. Canadian Journal of Plant Pathology 12 308-17.
  53. B. H. Ownley, B. K. Duffy, D. M. Weller. 2003. Identification and manipulation of soil properties to improve the biological control performance of phenazine-producing Pseudomonas fluorescens. Applied and Environmental Microbiology 69 3333-43.
  54. J. Van Veen, L. S. van Overbeek, J. D. van Elsas. 1997. Fate and activity of microorganisms introduced into soil. Microbiol. Mol. Biol. Rev. 61 121-135.
  55. J. Sorensen.1997. The rhizosphere as a habitat for soil microorganisms, in Modern soil microbiology, J. D. van Elsas, J. T. Trevors, E. M. H. Wellington (eds). Marcel Dekker, New York.
  56. E. A. Paul and F. E. Clark. 1989. Soil microbiology and biochemistry. Academic Press, San Diego. p234.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2017-06-30

Issue

Volume 1, Issue 6, CIBA - Conference Proceeding-2015

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Bensidhoum Leila, Rai Abdelwahab, Tabli Nassira, Kahouadji Nabila, Khaber Meriem ,Nabti Elhafid, " Biological Control of Botrytis cinerea by Bacillus sp. Strain S7LiBe Under Abiotic Stress, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 1, Issue 6, pp.7-14, CIBA - Conference Proceeding-2015.