The Relative Gene Expression of GAPDH in Mice Fed with a Short-Term High Fat Diet

Authors(6) :-Mariam DESSAY, Hasnaa MAKSOURI, Myriam RIYAD, Paolo AJMONE MARSAN, Khadija AKARID, Fatima CHEGDANI

The important role of nutrition in health and disease prevention is well recognized. The glyceraldehyde-3- dehydrogenase phosphate gene (GAPDH) is considered as a housekeeping gene: it encodes a key enzyme of the glycolysis, and seems to affect the metabolism of fatty acids. The purpose of this work was to study the relative expression of the GAPDH gene in mice subjected to a short-term high fat diet. The expression of the GAPDH gene has been analyzed in the livers of C57BL / 6N mice: 5 control mice and 5 mice subjected to a short term high-fat diet. The quantification of the mRNA has been performed by a real-time PCR using specific primers designated and validated in silico. The data have been analyzed by the REST software. The results showed a significant down expression (p < 0.005) of the GAPDH gene in mice subjected to high-fat diet. This suggests that a lipid diet may have an effect on the expression of the GAPDH gene. Thus the use of GAPDH as a reference gene should be reconsidered notably in gene expression studies.

Authors and Affiliations

Mariam DESSAY
University Hassan II Casablanca, Faculty of sciences Ain Chock Casablanca, Morocco
Hasnaa MAKSOURI
University Hassan II Casablanca, Faculty of Medicine and Pharmacy, Casablanca, Morocco
Myriam RIYAD
University Hassan II Casablanca, Faculty of Medicine and Pharmacy, Casablanca, Morocco
Paolo AJMONE MARSAN
Nutrigenomics Centre Universittą Cattolica Del Sacro Cuore, Piacenza, Italy
Khadija AKARID
University Hassan II Casablanca, Faculty of sciences Ain Chock Casablanca, Morocco
Fatima CHEGDANI
University Hassan II Casablanca, Faculty of sciences Ain Chock Casablanca, Morocco

GAPDH, high-fat Diet, Housekeeping Gene, Lipid Metabolism, Mice

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Publication Details

Published in : Volume 1 | Issue 6 | CIBA - Conference Proceeding 2015
Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 44-46
Manuscript Number : CIBA08
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Mariam DESSAY, Hasnaa MAKSOURI, Myriam RIYAD, Paolo AJMONE MARSAN, Khadija AKARID, Fatima CHEGDANI, " The Relative Gene Expression of GAPDH in Mice Fed with a Short-Term High Fat Diet, International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 1, Issue 6, pp.44-46, CIBA - Conference Proceeding-2015. Available at doi : 10.32628/CIBA08
Journal URL : http://ijsrst.com/CIBA08

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