Biofilm production and antibiotic susceptibility profiles of Staphylococcus strains isolates from urinary catheter at the university hospital center of Tlemcen (Algeria)

Authors(4) :-Ibtissem Kara Terki, Hafida Hassaine, Samia Bellifa, Did W.

Staphylococci are a major cause of infections associated with urinary catheterization and other medical devices. The ability of Biofilm production is an important step in the pathogenesis of these Staphylococci and depends on the expression of the icaADBC operon leading to the synthesis of a polysaccharide intercellular adhesion.
In this study , 44 staphylococci isolates obtained from urinary catheter at the university hospital center (UHC) of Tlemcen (North-West Algeria) were analyzed for detecting the presence or absence of the intercellular adhesion icaA and icaD genes by polymerase chain reaction, phenotypic biofilm production was examined by tissue culture plate (TCP) and Congo red agar (CRA) methods. 17of 44 isolates were shown to carry ica -specific DNA, 18 produced slime on CRA plates but only 8 produced biofilm spontaneously on the polystyrene surfaces. Staphylococci strains isolated from urinary catheter showed high levels of resistance to penicillin (98%) and gentamicine (75%). The data reported indicate an important role of ica genes, phenotypic variability of biofilm production and antibiotic multiresistance as virulence factors in staphylococcal from urinary catheters.

Authors and Affiliations

Ibtissem Kara Terki
Laboratory of Food, Biomedical and Environnemental Microbiology (LAMAABE) University of Tlemcen - Algeria
Hafida Hassaine
Laboratory of Food, Biomedical and Environnemental Microbiology (LAMAABE) University of Tlemcen - Algeria
Samia Bellifa
Laboratory of Food, Biomedical and Environnemental Microbiology (LAMAABE) University of Tlemcen - Algeria
Did W.
Laboratory of Food, Biomedical and Environnemental Microbiology (LAMAABE) University of Tlemcen - Algeria

Staphylococcus; Urinary catheter; Biofilm; ica operon; slime; TCP.

  1. Aricola CR, Commpaoccia D, Baldassari L, Donati ME, Gamberini S , Montanaro L .2005.   Detection of biofilm formation in Staphylococcus epidermidis from implant infections Comparisons of a PCR method that recognize the presence of ica genes with two classic phenotypic methods. J Biomed Mater Res.  76A (2): 425-430.
  2. Arciola CR, Baldassarri L, Montanaro L .2001. Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections. J Clin Microbiol .39:2151—2156.
  3. Brun-Buisson C .1994. Analyse critique des méthodes diagnostiques d’infection liée au cathéter sur matériel enlevé. Réan Urg.   3 : 343-6.
  4. Cafiso V, Bertuccio T, Santagati M, Campanile F, Amicosante G, Perilli MG, Selan L, Artini M, Nicoletti G, Stefani S .2004.   Presence of the ica operon in clinical isolates of Staphylococcus epidermidis and its role in biofilm production. Clin Microbiol Infect .10:1081-1088.
  5. Cerca N, Pier G B, Vilanova M, Oliveira R, Azeredo J. 2005. Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis. Research in Microbiology.  156 : 506–514
  6. Chaieb K, Mahdouania K,  Bakhrouf A .2005.  Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit. Journal of Hospital Infection .61: 225–230.
  7. Cho S, Naber K, Hacker J, Ziebuhr W .2002. Detection of the ica ADBC gene cluster and biofilm formation in Staphylococcus epidermidis isolates from catheter-related urinary tract infections. International Journal of Antimicrobial Agents. 19:570-/575.
  8. Chokr A,  Watier D, Eleaume H, Pangon B, Ghnassi JC, D Mack,  Jabbouri S.2006. Correlation between biofilm formation and production of polysaccharide intercellular adhesin in clinical isolates of coagulase-negative staphylococci. International Journal of Medical Microbiology .296: 381–388.
  9. Chokr A, Leterme D, Watier D,  Jabbouri S.2007.  Neither the presence of ica locus, nor in vitro-biofilm formation ability is a crucial parameter for some Staphylococcus epidermidis strains to maintain an infection in a guinea pig tissue cage model. Microbial Pathogenesis.  42: 94–97.
  10. Christensen GD, Simpson WA, Yonger JJ, Baddor LM, Barrett FF, Melton DM, Beachey EH .1985 .Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol .22:996-1006.
  11. Conlon KM, Humphreys H, O’Gara  JP. 2002. Regulation of icaR gene expression in Staphylococcus epidermidis. FEMS Microbiol . 216: 171–177.
  12. Cramton S, Gerke C,  Schnell N  F, Nichols W W, Gotz F .1999.  The Intercellular Adhesion (ica) Locus is Present in Staphylococcus aureus and is Required for Biofilm Formation.  Infection and immunity. 67(10):5427-5433.
  13. Cramton SE, Ulrich M, Gotz F. 2001. Anaerobic conditions induce expression of polysaccharide intercellular adhesin in Staphylococcus aureus and Staphylococcus epidermidis. Infect Immun .69:4079-4085.
  14. Diemond-Hernández B, Solórzano-Santos F, Leaños-Miranda B, Peregrino-Bejarano L, Miranda-Novales G. 2010.  Production of icaADBC-encoded polysaccharide intercellular adhesin and therapeutic failure in pediatric patients with staphylococcal device-related infections. BMC Infectious Diseases. 10:68.
  15. Dobinski S, Bartscht K, Mack D. 2002.  Influence of Tn917 insertion of transcription of the icaADBC operon in six biofilm-negative transposon mutants of Staphylococcus epidermidis . Plasmid.  47:10-17.
  16. Dobinsky S, Kiel K, Rohde H, Bartscht K, Knobloch JK, Horstkotte MA, Mack D. 2003. Glucose-related dissociation between icaADBC transcription and biofilm expression by Staphylococcus epidermidis: evidence for an additional factor required for polysaccharide intercellular adhesin synthesis. J Bacteriol. 185 : 2879–2886.
  17. Donlan RM.  2001. Biofilm formation: a clinically relevant microbiological process. Clin Infect Dis .33:1387–92.
  18. Duran N, Dogramaci Y, Ozer B, Demir C, Kalaci A .2010. Detection of adhesin genes and slime production among Staphylococci in orthopaedic surgical wounds. African Journal of Microbiology Research. 4(9): 708-715.
  19. El Mahallawy H A, Samah A. El-Wakil LM, Abeer K, Morcos H. 2009. Clinical implications of icaA and icaD genes in coagulase negative Staphylococci and S. aureus bacteremia in febrile neutropenic pediatric cancer patients. Pediatric Blood and Cancer .52(7): 824-828.
  20. Espinassea F, Pageb B, Cottard-Boullea B .2010.  Risques infectieux associés aux dispositifs médicaux invasifs. Revue francophone des laboratoires.   426 :51-63.
  21. Fitzpatrick F, Humphreys H, Smythy E, Kennedy CA, O'Gara GP . 2002 . Environmental regulation of biofilm formation in intensive care unit isolates of Staphylococcus epidermidis. Journal of Hospital Infection .42: 212-218.
  22. Freeman DJ, Falkner FR, Keane CT .1989. new method for detecting slime    production by coagulase-negative staphylococci. J Clin Pathol. 42:872-874.
  23. Gad G, El-Feky M, El-Rehewy M, Hassan M, Abolella H, Abd El-Baky R. 2009.   Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients.  J Infect Dev Ctries.  3(5):342-351.
  24. Gerke C, Kraft A, Sussmuth R .1998. Characterization of the N-acetylglucosaminyl transferase activity involved in the biosynthesis of the Staphylococcus epidermidis polysaccharide intercellular adhesion . J Biol Chem. 273:86-93.
  25. Gilbert P, Maira-Litran T, McBain AJ, Rickard AH, Whyte F .2002. the physiology and collective recalcitrance of microbial biofilm communities. Advances in Microbial Physiology .46: 203-256.
  26. Gotz F .2002. Staphylococcus and biofilms. Mol Microbiol. 43:1367-1378.
  27. Hoyle B D, Costerton J W .1990. Bacterial resistance to antibiotics: the role of biofilms. Prog Drug Res.    37:91–105.
  28. Jain A, Agarwal A .2009. Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci. J Microbiol Methods. 76:88-92
  29. Johannes KM,  Knobloch MA, Horstkotte H,  Dietrich M .2002. Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. Med Microbiol Immunol. 191: 101–106.
  30. Macka D, Beckerb P, Chatterjeec I, Dobinskya S, Knoblocha J K M, Petersb G, Rohdea H, Herrmannc M .2004.   Mechanisms of biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus: functional molecules, regulatory circuits, and adaptive responses.  International Journal of Medical Microbiology. 294: 203–212.
  31. Martín-López JV, Pérez-Roth E,   Díez Gil FCMO,  Batista N,    Morales M ,  Méndez-Álvarez S. 2002 . Detection of Staphylococcus aureus clinical isolates harboring the ica Gene cluster needed for biofilm establishment. J Clin Microbiol. 40(4): 1569–1570.
  32. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A . 2006.  Detection of biofilm formation among the clinical isolates of Staphylococci: An evaluation of three different screening methods.  Indian Journal of Medical Microbiology  .24 (1) :25-9
  33. Mempel M, Feucht H, Ziebuhr W, Endres M, Laufs R, Gruter L .1994. Lack of mecA transcription in slime-negative phase variants of methicillin-resistant Staphylococcus epidermidis . Antimicrob Agents Chemother .38:1251-/5.
  34. Oliveira A, de Lourdes RS, Cunha M .2010. Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci. BMC Research Notes. 3:260
  35. Otto M .2008 .Staphylococcal Biofilms.Microbiol Immunol.322: 207–228.
  36. Seif El-Din S, El-Rehewy M S, Ghazaly M M, Abd-Elhamid M H .2011. Biofilm Formation by Blood Stream Staphylococcal Isolates from Febrile Pediatric Cancer Patients at South Egypt Cancer Institute. Journal of American Science.7 (1): 674-686.
  37. Soussy CJ .2010 .Comité de l’antibiogramme de la Société française de microbiologie. Édition janvier  .33–35.   
  38. Touati A , Achour W,  Abbassi MS,  Ben Hassen A .2007  . Detection of ica genes and slime production in a collection of Staphylococcus epidermidis strains from catheter-related infections in neutropenic patients. Pathologie Biologie  .55: 277–282.
  39. Wang L, Yu F, Yang L, Li, X Zhang Q, Zeng Y, Xu Y .2010 .Prevalence of virulence genes and biofilm formation among Staphylococcus aureus clinical isolates associated with lower respiratory infection. African Journal of Microbiology Research  . 4(23): 2566-2569.
  40. Ziebuhr W, Heilmann C, Gotz F .1997. Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infect Immun .65:890-6.
  41. Ziebuhr, W, Krimmer, V, Rachid S, Lossner, I, Gotz F, Hacker J .1999. A novel mechanism of phase variation of virulence in Staphylococcus epidermidis: evidence for control of the polysaccharide intercellular adhesin synthesis by alternating insertion and excision of the insertion sequence element IS256. Mol Microbiol. 32 : 345–356.

Conflict of interest: No conflict of interest is declared.

Publication Details

Published in : Volume 1 | Issue 6 | CIBA - Conference Proceeding 2015
Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 77-85
Manuscript Number : CIBA14
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Ibtissem Kara Terki, Hafida Hassaine, Samia Bellifa, Did W., " Biofilm production and antibiotic susceptibility profiles of Staphylococcus strains isolates from urinary catheter at the university hospital center of Tlemcen (Algeria)", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 1, Issue 6, pp.77-85, CIBA - Conference Proceeding-2015.
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