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Biofilm production and antibiotic susceptibility profiles of Staphylococcus strains isolates from urinary catheter at the university hospital center of Tlemcen (Algeria)

Authors(4) :-Ibtissem Kara Terki, Hafida Hassaine, Samia Bellifa, Did W.

Staphylococci are a major cause of infections associated with urinary catheterization and other medical devices. The ability of Biofilm production is an important step in the pathogenesis of these Staphylococci and depends on the expression of the icaADBC operon leading to the synthesis of a polysaccharide intercellular adhesion.
In this study , 44 staphylococci isolates obtained from urinary catheter at the university hospital center (UHC) of Tlemcen (North-West Algeria) were analyzed for detecting the presence or absence of the intercellular adhesion icaA and icaD genes by polymerase chain reaction, phenotypic biofilm production was examined by tissue culture plate (TCP) and Congo red agar (CRA) methods. 17of 44 isolates were shown to carry ica -specific DNA, 18 produced slime on CRA plates but only 8 produced biofilm spontaneously on the polystyrene surfaces. Staphylococci strains isolated from urinary catheter showed high levels of resistance to penicillin (98%) and gentamicine (75%). The data reported indicate an important role of ica genes, phenotypic variability of biofilm production and antibiotic multiresistance as virulence factors in staphylococcal from urinary catheters.

Ibtissem Kara Terki, Hafida Hassaine, Samia Bellifa, Did W.
Staphylococcus; Urinary catheter; Biofilm; ica operon; slime; TCP.
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Conflict of interest: No conflict of interest is declared.

Publication Details
  Published in : Volume 1 | Issue 6 | CIBA - Conference Proceeding 2015
  Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 77-85
Manuscript Number : CIBA14
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Ibtissem Kara Terki, Hafida Hassaine, Samia Bellifa, Did W., "Biofilm production and antibiotic susceptibility profiles of Staphylococcus strains isolates from urinary catheter at the university hospital center of Tlemcen (Algeria)", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 1, Issue 6, pp.77-85, CIBA - Conference Proceeding-2015
URL : http://ijsrst.com/CIBA14