Isolation and Enrichment of Microbes for Degradation of Diesel Oil

Authors

  • Pooja Pandey  Department of Biotechnology, School of Science, JECRC University, Jaipur, Rajasthan, India
  • Hardik Pathak  Department of Biotechnology, School of Science, JECRC University, Jaipur, Rajasthan, India
  • Saurabh Dave  Department of Chemistry, School of Science, JECRC University, Jaipur, Rajasthan, India

Keywords:

Environmental pollution, Petroleum, Microbes, Enrichment and Biodegradation

Abstract

Oil refinery proves to be chief source of environmental pollution, which leads to human health hazards and damage soil surroundings. The present investigation aims to isolate petroleum tolerant and degrading microbes from oil contaminated soil of oil refinery, which later be used in environmental biotechnology as remediating mechanisms. Bushnell Haas Media (BHM) supplemented with diesel oil as carbon and energy source, used for isolation of oil degrading bacteria. The degraders were analyzed by streak plate method and bacteria were isolated by the selective enrichment culture plate technique and the biodegradation potential was determined.

References

  1. Plohl K, Leskovsek H, Bricelj M. (2002). Biological degradation of motor oil in water. Acta Chim. Slovenica. 49: 279-289.
  2. Geetha SJ, Sanket JJ, Kathrotiyab S. (2013). Isolation and characterization of hydrocarbon degrading bacterial isolate from oil contaminated sites APCBEE Procedia 5, 237 ? 241
  3. Teli N, Verma D, Gavankar R, Bhalerao S. (2013). Isolation, Characterization and Identification of Diesel Engine Oil Degrading Bacteria from Garage Soil and Comparison of their Bioremediation Potential. International Research Journal of Environment Sciences. 2(2): 48-52.
  4. Baker, JM. (1982). Mangrove swamps and the oil industry. Environment pollution bulletin. 12.
  5. Abdel-El-Haleem D, Moawad H, Zaki E, Zaki S. (2002) Molecular characterization of phenol-degrading bacteria isolated from different Egyptian ecosystems. Microb. Ecol. 43: 217-224.
  6. Samanta KS, Singh OV, Jain RK. (2002). Polycyclic aromatic hydrocarbons: environmental pollution and bioremediation. Trends Biotechnol. 20(6): 243-248.
  7. Wilson, SC. and Jones, KC. (1993) Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons, Environmental pollution. 81: 229- 249.
  8. Quatrini P, Scaglione G, De Pasquale C, Reila S, Puglia AM. (2008). Isolation of Gram-positive n-alkane degraders from a hydrocarbon contaminated Mediterranean shoreline. J Appl Microbiol. 104: 251- 259.
  9. Nwinyi OC. (2014). Characterization of diesel degrading bacterial species from contaminated tropical ecosystem. Braz. arch. biol. Technol Curitiba. 57(5):789-796.?
  10. Chikere CB, Okpokwasili GC, Chikere BO. (2009). Bacterial diversity in a tropical crude oil-polluted soil undergoing bioremediation. Afr J Biotech. 8(11):2535-2540.
  11. Bushnell, L.D. and H.F. Haas. (1941). The utilization of certain hydrocarbons by microorganisms. Journal of Bacteriology. 41: 653-673.
  12. Bharathi, S., and N. Vasudevan. 2001. Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from petroleum contaminated soil. Environ. Int., 26:413 413.
  13. Alessandra CO, Chagas-Spinelli Mario TK, Edmilson S, de Lima SG. (2012). Bioremediation of a tropical clay soil contaminated with diesel oil. J Environ Management. 113: 510-516.
  14. Akhavan Sepahi, I. Dejban Golpasha, M. Emami, A. M. Nakhoda, Iran. (2008). J. Environ. Health. Sci. Eng., 5(3), 149-154.
  15. Jirasripongpun K. (2002). The characterization of oil-degrading microorganisms from lubricating oil contaminated (scale) soil. Lett Appl Microbiol. 35: 296-300.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2018-04-30

Issue

Volume 4, Issue 5, March-April-2018

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Pooja Pandey, Hardik Pathak, Saurabh Dave, " Isolation and Enrichment of Microbes for Degradation of Diesel Oil, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 5, pp.1281-1284, March-April-2018.