Home > Archives > IJSRST11845496 IJSRST-Library

Pathogenicity of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina : Hyphomycetes) against the Odontotermes obesus (Rambur) workers (Isoptera : Termitidae) under laboratory conditions

Authors(1) :-Anjana Intodia

Odontotermes obesus (Rambur) (Isoptera: Termitidae) is a common termite pest found in almost all crop plants in South East part of Rajasthan (India).It causes considerably economic losses in tropical and subtropical areas and occurrence of termites in the crop field has become a major threat to farmers. Presently, the control methods mostly swing towards chemical that has led to high cost, persistence and adverse effects to the environment. Presently, emphasis has been given on safer bio control agents like fungi, protozoans, bacteria and virus etc. that are environmental friendly. The present investigation is an attempt to control Odontotermes obesus (R) workers with pathogenic fungus Beauveria bassiana . The pathogenicity of Beauveria bassiana (Balsamo) was tested against the worker termite at four different concentrations (4.5 x 108, 4.5 x 107, 4.5 x 106 and 4.5 x 105 conidia/ml.). Complete population check was observed after 8 days of post inoculation at 4.5 x 108 conidia / ml with LC50 value of 8.67 X 107 conidia / ml. under laboratory conditions. ANOVA further supported the significance of the result (F= 89.61; df=3 and 8, P < 0.05).  LT50 and LT90 for highest dose (4.5 x 108 conidia / ml) were 2.55 days and 5.15 days respectively with inverse correlation with concentrations. Various developmental changes such as abnormal appendages, fragile body, shrunken body, body covered with fungal mycelium were also indicative of chitin inhibitory nature of fungus thus revealing two fold effect on the treated insects. Thus the result shows that mycoinsecticide Beauveria bassiana has a great potential to control the termite and reduce the burden of chemical insecticides on the environment.
Anjana Intodia
Beauveria bassiana, Odontotermes obesus, mortality, ANOVA, Entomopathogenic fungus, mycoinsectisides,
  1. Abbott W S. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 1925; 265-267.
  2. Ahmed, S, Ashraf, M.R., Hussain, M A, Riaz, M A. Pathogenicity of isolates of Metarhizium anisopliae from Gujranwala (Pakistan) against Coptotermes heimi (Wasmann) (Isoptera: Rhinotermitidae). Int J Agric Biol, 11, 2009; 707–711.
  3. Alves, S B, Almeida, J E M, Moino Jr., A, Stimac, J L Pereira, RM. Uso de Metarhizium anisopliae and Beauveria bassiana no controle de Cornitermes cumulans (Kollar, 1832) em pastagens. Australia, 1995; 44.
  4. Carruthers, R I, Soper, R S.Fungal diseases,. In; J. R. Fuxa & Y.Tanada Eds.], Epizootiology of insect diseases. John Wiley and Sons, New York, 1987; 357–416.
  5. Changjin D,Zhang J, Huang H, Chen W and Hu Y Pathogenicity of a new China variety of Metarhizium anisopliae (M. Anisopliae var. Dcjhyium) to subterranean termite Odontotermes formosanus.Microbiological Research vol. 164. 2009; 27-35.
  6. Chouvenc Thomas, Su Nan-Yao, Grace, J Kenneth. Fifty years of attempted biological control of termites-Analysis of a failure, Biological Control .59 (2): 2011;69–82.
  7. Creffield, JW. Wood-destroying Insects, Wood Borers and Termites, CSIRO Publishing, Collingwood, Dekker, Inc. Basel, Switzerland, 1996; 211-222 PP.
  8. Delante K M, Grace J K, Tome C H M. Potential use of pathogenic fungi in baits to control directorymcp/directory.html. 1995.
  9. Dong C, Zhang J, Huang H, Chen W, Hu, Y. Pathogenicity of new China variety of Metarhizium anisopliae (Manisopliae) to subterranean termite Odontotermes formosanus. Microbiol Res 164: 2009; 27–35.
  10. Duncan D B. Multiple Range and Multiple F.-Test. Biometrics 11: 1955; 1-12.
  11. Fuxa JR. Ecological considerations for the use of Entomopathogens in IPM. Annual Review of Entomology, 32: 1987; 225-51.
  12. Grace JK. Biological control strategies for suppression of termites. J. Agric. Entomol. 14: 1997; 281-289.
  13. Jung HS, Lee HB, Kim K, Lee EY. Selection of Lecancillium strains for aphid (Myzus persicae) control. Korean J. Mycol., 34: 2006; 112- 118.
  14. Karthikeyan K, Jacob S. Biological efficacy of Beauveria bassiana against rice blue beetle, Leptispa pygmaea Baly (Coleoptera: Chrysomelidae) . International Research Journal of Plant Science, 1(2): 2010; 026-033.
  15. Khetan, SK Mycoinsecticides. In: Khetan, S.K. (ed.) Microbial pest control. 2001; 55 PP.
  16. Lacey LA, Wraight SP, and Kirk AA Entomopathogenic fungi for control of Bemisia spp.foreign exploration, research and implementation. In: Classical Biological Control of Bemisia tabaci in the USA: A Review of Interagency Research and Implementation. J.K.Gould, K.Hoelmer, J Goolsby (eds.), Vol 4 'Progress in Biological Control' (H.Hokkanen, series editor), springer, Dorcrecht. 2008; 33-69.
  17. Liu H, Skinner M, Parker BL,Brownbridge M. Pathogenicity of Beauveria bassiana, Metrhazium anisopliae -(Deuteromycotina: Hyphomycetes) and other entomopathogenic fungi against Lygus lineolaris (Hemiptera: Miridae). J. Econ. Entomol., 95: 2002; 675–81.
  18. McCoy CW, Shapiro WDI and Ducan LW Application and Evaluation of Entomopathogens .Application and Evaluation of Entomopathogens for Citrus Pest Control. In: Field Manual of Techniques in Invertebrate Pathology; application and Evaluation of Pathogens for insects and other invertebrate Pests (Lacy,L.A. and H.K. Kaya, Eds.),Kluwer Academic publishers, Dordrecht. 2000; 33.
  19. Milner RJ Staples JA, Hartley TR, Lutton GG, Driver F, Watson WAL. Occurrence of Metarhizium anisopliae in nests and feeding sites of Australian termites. Mycological Research 102, 1998; 216- 220.
  20. Quasim M. Termites and Microbial biological control strategies.South Asia of multidisciplinary studies. Vol. 1 No. 6; 2015.
  21. Pardeshi MK, Kumar D,Bhattacharya A K Termite (Insecta: Isoptera) Fauna of some agricultural crops of Vadodara, Gujarat, India.Rec. Zool.Surv. India, 110. 2010; 47-59.
  22. Pearce, M.J.. Termites-Biology and Pest Management. CAB International, London; 1997;172.
  23. Pik-Kheng H Choon-Fah J, Bong KJ,Amartalingam R. Evaluation of Metarhizium anisopliae var. anisopliae (Deuteromycotina: Hyphomycete) isolates and their effects on subterranean termite Coptotermes curvignathus (Isoptera: Rhinotermitidae) American. J Agric Biol Sci 4(4): 2009; 289–297.
  24. Quesada M E, Maranhao E A A, Garcia P V,Alvarez C S Selection of Beauveria bassiana isolates for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements, and toxicogenic activity. Biol. Control, 36: 2006; 274 -287.
  25. Quesada-Moraga E, Navas-Cortes JA, Maranhao EAA, Ortiz-Urquiza A ,SantiagoAlvarez C. Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. Mycological Research 111: 2007; 947-966.
  26. Roonwal ML, Chhotani OB.Fauna of India- Isoptera(termites). ZSI .I. 1989; 672.
  27. Rosengaus RB, Traniello JFA. Pathobiology and disease transmission in dampwood termites Zootermopsis angusticollis (Isoptera: Termopsidae)] infected with the fungus Metarhizium anisopliae (Deuteromycotina: Hypomycetes). Sociobiology 30. 1997; 185–195.
  28. Sattar A,Salihah Z. Detection and control ofsubterranean termites. In: Technologies for Sustainable Agriculture (Ed.). Proceed. Natl. Workshop, Sept. 24-26 NIAB, Faisalabad, Pakistan. 2001; 195-198.
  29. Shah PA, Tymon A,Pell JK . Conservation biocontrol with mycopathogens:Erynia neoaphidis and agricultural field margins. Proceedings of the XXXIV Annual Meeting of the Society for Invertebrate Pathology, Noordwijkerhout, The Netherlands. 2001
  30. Shimazu M A. novel technique to inoculate conidia of entomopathogenic fungi and its application for investigation of susceptibility of the Japanese pine sawyer, Monochamus alternatus, to Beauveria bassiana. Appl. Entomol. Zool. 39: 2004; 495-490.
  31. Singha D, Singha B,Dutta B K. Ultrastructural Details of the Morphological Changes in Termite (Microtermes obesi Holmgren) Pest of Tea Exposed to Entomopathogenic Fungi in vitro. Assam University Journal of Science & Technology :Biological and Environmental Sciences( 5) I:2010;100-104.
  32. Smyth DS . Insect Cell: Their structure and function. Edinburgh: Oliver & Boyd. 1968; 262-269.
  33. Varshney A K . Applied Entomology Insecticidal Methods of Pest Control: Household Pests-Termites (The White Ants). 2004; 11-12.
  34. Wright M S,Osbrink W L A ,Lax A R. Potential of Entomopathogenic Fungi as Biological Control Agents against the Formosan Subterranean Termite. Agricultural Applications in Green Chemistry: 2004; 173–188.
Publication Details
  Published in : Volume 1 | Issue 1 | March-April 2015
  Date of Publication : 2015-04-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 95-104
Manuscript Number : IJSRST11845496
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Anjana Intodia, "Pathogenicity of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina : Hyphomycetes) against the Odontotermes obesus (Rambur) workers (Isoptera : Termitidae) under laboratory conditions ", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 1, Issue 1, pp.95-104, March-April-2015
URL : http://ijsrst.com/IJSRST11845496