In the current situation heavy metal contamination is becoming a serious threat for plants, animals, humans and whole nature, the main sources of soil and water pollution are solid waste and waste water sometimes with extremely toxic level of hazardous substances. To manage these wastes, the decomposition of it by the potent microbial action is the only safer, natural and cheap way. Because all the microbes are isolated from the nature and being studied by the scientist weather they donít have any type of negative impact on soil, plant, animal, humans or nature in any way. The present study deals with isolation, identification and characterization of heavy metal resistant bacteria was isolated from different metal dumping sites collected in and around Hosur. Initially, a total of 27 isolates were screened from the effluent after screening experiments. The five isolates were selected based on high level of heavy metal and antibiotic resistances. On the basis of morphological, biochemical analysis revealed that, the isolates were authentically identified as Acinetobacter pittii, Escherichia coli, Fictibacillus nanhaiensis, Lysinibacillus xylanilyticus and Planococcus maritimus. The identified isolates were resistant to Aluminium nitrate (Al(No3)3), Calcium chloride (CaCl2), Nickel sulphate (NiSo4), Cobalt chloride (CoCl2), Mercury chloride (HgCl2), Potassium dichromate (K2Cr2O7), Zinc sulfate (ZnSo4) and Copper(II) sulfate (CuSO4). The minimum inhibitory Concentration (MIC) of the effluent isolates was determined in solid media. The multiple metal resistances of these isolates were also associated with Antibiotics Ampicillin, Penicillin, Bacitracin, Novobiacin, Erythromycin, Methicillin, Rifampicin, Clindamycin, Linezolid, Imipenem, Azithromycin and Trimetholinin. The identified heavy metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sites.
Mahilarasi A, Veeramanikandan Veeramani, Jaianand Kannaiyan, Balaji Paulraj
Antibiotics, Bio-Remediation, Bacteria, Heavy Metals