Capability of Bioorganic Fertilizer Pseudomonas GanoEB3 for Suppressing Basal Stem Rot Disease in Oil Palm Seedlings

Authors

  • AW Nurul Isma  Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  • AS Idris  Ganoderma and Diseases Research for Oil Palm Unit (GANODROP), Division of Biology, Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, Kajang, Selangor, Malaysia
  • N Rosimah  Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  • O Hishamuddin  Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  • A Mohd. Puad  Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

Keywords:

Basal stem rot, endophytic bacteria, Pseudomonas GanoEB3, bioorganic EFB Pseudomonas GanoEB3, Bioorganic RS Pseudomonas GanoEB3, pathological study

Abstract

Oil palm is one of the important crops in Malaysia. Basal Stem Rot (BSR) disease is the biggest threat for oil palm production in Malaysia caused by Ganoderma boninense which had been causing a huge damage for the oil palm industry in Malaysia. The objective of this study was to develop bioorganic fertilizer containing Pseudomonas GanoEB3 for suppressing basal stem rot disease in oil palm seedlings. Endophytic bacteria, Pseudomonas GanoEB3 was isolated from healthy oil palm roots and cultured on nutrient agar media. A suspension containing 108 CFU/mL of the bacteria cells being mixed with vermiculite powder and stored at room temperature. The vermiculite powder containing Pseudomonas GanoEB3 has been formulated with Bioorganic EFB and Bioorganic RS. Pathological analysis showed that Bioorganic EFB Pseudomonas GanoEB3 (T3) and Bioorganic RS Pseudomonas GanoEB3 (T4) have a good potential in inhibiting basal stem rot disease in oil palm seedlings. After eight months of experiment, oil palm seedlings treated with Bioorganic EFB Pseudomonas GanoEB3 and Bioorganic RS Pseudomonas GanoEB3 resulted the reduced percentage for following parameters; disease incidence (DI) for T3 (40%) and T4 (50%), area under the disease progress curve (AUDPC) for T3 (42 units2) and T4 (76 units2), disease severity of foliar index (DSFI) for T3 (37.5%) and T4 (45%), disease severity of bole index (DSBI) for T3 (40%) and T4 (47.5%), disease severity of root index (DSRI) for T3 (55%) and T4 (52.5%) and dead seedlings for T3 (30%) and T4 (40%) compared with control treatment (T2). It shows that both treatments have a good potential in inhibiting BSR disease in oil palm seedlings. This study revealed that Bioorganic EFB and Bioorganic RS containing Pseudomonas GanoEB3 are suitable as an effective biological control agent for suppressing BSR disease in oil palm seedlings.

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International Journal of Scientific Research in Science and Technology

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Published

2015-12-25

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Volume 1, Issue 5, November-December-2015

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Research Articles

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[1]
AW Nurul Isma, AS Idris, N Rosimah, O Hishamuddin, A Mohd. Puad, " Capability of Bioorganic Fertilizer Pseudomonas GanoEB3 for Suppressing Basal Stem Rot Disease in Oil Palm Seedlings, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 1, Issue 5, pp.264-274, November-December-2015.