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Acrylamide - induced acute nephrotoxicity in Rats

Authors(1) :-Keivan Jamshidi

Acrylamide (ACR) has been shown to cause neurotoxic effects in humans and neurotoxic, genotoxic, reproductive, and carcinogenic effects in laboratory animals. To investigate the nephrotoxic effect of Acrylamide (ACR), 50 adult male rats (Wistar, approximately 250 g) housed in polycarbonate boxes as 5 per each, and randomly assigned in 5 groups including 4 exposure groups as A, B, C, and D groups of rats (10 rats per exposure group., total) and were exposed to 0.5, 5, 50, 100 mg/kg ACR per day×11days i.p. respectively. The remaining 10 rats were housed in group (E) as control group. Control rats received daily i.p. injections of 0.9% saline (3ml/kg). On day 12, four rats, were randomly selected, perfused , dissected and proper samples were collected from their kidneys. Results of histopathological studies based on H&E technique did show no morphologic changes in kidneys of rats belong to groups A, B and E, while moderate to severe morphologic changes including glomerular hypercellularity, global pattern of proliferative glomerulonephritis, occupation of capsular space, tubular cell swelling and hyaline cast formation, were observed in different stained sections obtained from the kidneys of rats belong to group, C, and D. This finding, beside neurotoxic, reproductive and carcinogenic effects, seems to indicate for the first time another important aspect of toxic effect of ACR, i.e., acute nephrotoxicity.
Keivan Jamshidi
Acrylamide, nephrotoxicity, glomerulonephritis, rats
  1. LoPachin RM, Balaban C.D., Ross,J.F.,2003. Acrylamide axonopathy revisited. Toxicol  Appl  Pharmacol .188,135-153.
  2. Kirsi Annolaa, Vesa Karttunena, Pekka Keski-Rahkonenb, P ivi Myllynenc, Dan Segerb ckd, Seppo Heinonene, Kirsi V h kangasa .2008.Transplacental transfer of acrylamide and glycidamide are comparable to that of antipyrine in perfused human placenta., Toxicology Letters 182, 50–56
  3. LoPachin R M, 2004: The Changing View of Acrylamide Neurotoxicity  NeuroToxicology 25, 617–630  Acrylamide axonopathy revisited Toxicology and Applied Pharmacology 188 (2003) 135–153
  4. Tyla W, Melissa C M, Christina B. Myersa, W P. Rossa M A. 2000: Friedmanb Relationship between acrylamide reproductive and neurotoxicity in male rats Rochelle. Reproductive Toxicology 14 147–157
  5. Shan-xia Li , Ning Cui b, Cui-li Zhang , Xiu-lan Zhao, Su-fang Yu , Ke-qin Xie 2006: Effect of subchronic exposure to acrylamide induced on the expression of bcl-2, bax and caspase-3 in the rat nervous system . Toxicology 217, 46–5
  6. Hao Wanga, Pan Huanga, Tietao Lie, Jian Li, Reinhold J. Hutzc, Kui Li, Fangxiong Shia 2010: Reproductive toxicity of acrylamide-treated male rats, Reproductive Toxicology 29 225–230
  7. Hye-Jin Y, Sang-Hyun L, Yong J, Jin-Hyang C, Dong-Un H, Chanhee C, Mun-Han L, Chang-Hoon H. 2005: Toxicological effects of acrylamide on rat testicular gene expression profile,Reproductive Toxicology 19 527–534
  8. Jamshidi K, Tiraihi T, Sohrabi Haghdoost I  2009: Light and electron microscopy study of the neuropathologic effects of different doses of acrylamide monomer on CNS of rat. Toxicology Letters, Volume 189, Supplement 1, 13 September  Page S163.
  9. Jamshidi K and Panahi S. 2011.
  10. Tyl RW, Friedman MA. Effects of acrylamide on rodent reproductive performance.Reprod Toxicol 2003;17:1–13.
  11. Spencer, P.S., Schaumburg, H.H., 1974a. A review of acrylamide neurotoxicity. Part I. Properties, uses and human exposure. Can. J. Neurol. Sci. 1, 151–169.
  12. LoPachin .R M. The Changing View of Acrylamide Neurotoxicity * NeuroToxicology 25 (2004) 617–630
  13. Collins, J.J., Swaen, G.M., Marsh, H.M.D., Utidjian, J.C.,
  14. Marsh, G.M., Lucas, L.J., Youk, A.O., Schall, L.C., 1999. Mortality patterns among workers exposed to acrylamide:1994 follow up. Occup. Environ. Med. 56, 181–190.
  15. Sakamoto, J., Hashimoto, K., 1986. Reproductive toxicity of acrylamide and related compounds in mice—effects on fertility and sperm morphology. Arch. Toxicol. 59, 201–205.
  16. Sublet VH, Zenick H, Smith MK. Factors associated with reduced fertility and implantation rates in females mated to acrylamide-treated rats. Toxicology 1989;55:53– 67.
  17. Tyl RW, Marr MC, Myers CB, Ross WP, Friedman MA. Relationship between acrylamide reproductive and neurotoxicity in male rats. Reprod Toxicol 2000;14:147–57.
  18. Yang, H.-J. et al., 2005. Toxicological effects of acrylamide on rat testicular gene expression profile. Reprod. Toxicol. 19, 527–534.
  19. McGavin MD, Zachary JF 2007: Patholgic Basis of Veterinary Disease. Mosby International. China.  628-631p
  20. Hickey E J, Raje R R, Reid V E, Gross S M, and Ray S D  2001: DICLOFENAC INDUCED IN VIVO NEPHROTOXICITY MAY INVOLVE OXIDATIVE STRESS-MEDIATED MASSIVE GENOMIC DNA FRAGMENTATION AND APOPTOTIC CELL DEATH. Free Radical Biology & Medicine, Vol. 31, No. 2, pp. 139–152
  21. Ishikawa A, Suzuki K, and Fujita K 1999:  Mechanisms of Cyclosporine-Induced Nephrotoxicity Transplantation Proceedings, 31: 1127–1128
  22. Blachley JD, Hill JB. Renal and electrolyte disturbances associated with cisplatin. Ann Int Med 1981;95:628–32.
  23. Weijil NI, Cleton FJ, Osanto S. Free radicals and antioxidants in chemotherapy-induced toxicity. Cancer Treat Rev 1997;23:209–40.
Publication Details
  Published in : Volume 1 | Issue 5 | November-December 2015
  Date of Publication : 2015-12-25
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 286-293
Manuscript Number : IJSRST151428
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Keivan Jamshidi, "Acrylamide - induced acute nephrotoxicity in Rats", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 1, Issue 5, pp.286-293, November-December-2015
URL : http://ijsrst.com/IJSRST151428