Impact of an atmosphere rich in sulfur dioxide on a foliose lichen species Flavoparmelia caperata (L) Hale from the El Kala National Park (northeast Algerian)

Authors(4) :-Monia Serradj Ali Ahmed, Zineeddine Boumedris, Ali Tahar, Mohamed Reda Djebar

Lichens are often exposed in their natural habitat to anthropogenic pollutants, including those from vehicles and industrial and agricultural activities. In the absence of outer protective barrier, such that the waxy cuticle has evolved in vascular plants. The thallus of these organisms is sensitive to the penetration of suspended substances, some of which are essential for the functioning of cells, while others can be toxic. Accordingly, lichens can disappear within and around urban and industrial areas. Lichens can be used for early detection of stress caused by air pollution.
To better characterize the impact of SO2 pollution in lichens. We began our study by highlighting the accumulation of sulfur dioxide by the thallus of foliose species Flavoparmelia caperata (L) Hale using the immersion method designed by Deruelle and Lallemand, which involves immersing lichens in SO2 -saturated atmosphere at different concentrations . Thereafter, we performed morphological observations and measurements of some physiological parameters (chlorophyll, total protein and as biomarkers of oxidative stress such as antioxidant enzymes system).
Our results indicate physiological and biochemical morphological alterations of lichens exposed to SO2, including
• discolorations and necrosis weaknesses frond splitting them into small clusters
• Disturbances contents chlorophylls (a, b and (a + b) )
• Activation detoxification systems ( APX , CAT and GPX ) ;
• Increased levels of proteins.

Authors and Affiliations

Monia Serradj Ali Ahmed
Biology Department, Badji Mokhtar University, Laboratory of Plant Biology and Environment, Annaba, Algeria
Zineeddine Boumedris
Biology Department, Badji Mokhtar University, Laboratory of Plant Biology and Environment, Annaba, Algeria
Ali Tahar
Biology Department, Badji Mokhtar University, Laboratory of Plant Biology and Environment, Annaba, Algeria
Mohamed Reda Djebar
Biology Department, Badji Mokhtar University, Laboratory of Plant Biology and Environment, Annaba, Algeria

Lichen, Biomarkers, SO2, Oxidative Stress, Pollution.

  1. Le Blanc F., 1969. Epiphytes And Air Pollution. Air Pollut., Proc.Eur.Congr. Influence Air Pollut. Plant Anim., 1st, 1968, Pp.211-221.
  2. Marthe Bégin-Robitaille, 1978. Pollution Atmosphérique Et Végétation Épiphytique Dans La Région De La Capitale Nationale Du Canada. Thèse Présentée À l'Université d'Ottawa Pour L'obtention Du Diplôme De Maîtrise En Sciences (Biologie).
  3. Garrec J.Pierre.,  Van Haluwyn C, 2002. Biosurveillance Végétale De La Qualité De L'air, Concepts, Méthodes Et Applications. Ed Tec Et Doc - Lavoisier.
  4. Markert, B.A., Breure, A.M. Et Zechmeister, H.G. (2003). Definitions, Strategies And Principles For Bioindication/Biomonitoring Of The Environment. Bioindicators And Biomonitors: Principles, Concepts And Applications, B.A. Markert, A.M. Breure, H.G. Zechmeiter, Editors. (Oxford : Elsevier Science Limited), Pp. 3-39
  5. Deruelle S., Lallemant R, 1983. Les Lichens Témoins De La Pollution. Thèmes Vuibert Université De Biologie, Paris. 
  6. Jean-Pierre Gavériaux, 2008. Association Française De Lichénologie AFL - Les Champignons Lichénisés De France
  7. Plan De Gestion PNEK 2005, Algérie
  8. Bradford M, 1976. A Rapid And Sensitive Method For The Quantification Of Microgram Quantities Of Protein Utilizing The Principle Of Protein-Dye Binding. Anal. Biochem; 72: 248-254.
  9. Holden M, 1975. Chlorophylls In Chemistry And Biochemistry Of Plant Pigment.2nd Edition  Academie Press, New York, Pp: 133.
  10. Arnon DI, 1949. Copper Enzymes In Isolated Chloroplasts. Polyphenoloxidase In Beta Vulgaris. Plant Physiol 24: 1–15
  11. Loggini B., Scartazza A., Brugnoli E & Navari-Izzo F, 1999. Antioxidative Defence System, Pigment Composition And Photosynthetic Efficiency In Two Wheat Cultivars Subjected To Drought. Plant Physiology 119: 1091-1100.
  12. Nakano Y & Asada K, 1987. Purification Of Ascorbate Peroxidase In Spinach Chloroplasts: Its Inactivation In Ascorbate Depleted Medium And Reactivation By Mono Dehydro Ascorbate Radical. Plant Cell Physiol; 28: 131-140.
  13. Cakmak I., Horst W.J, 1991. Effect Of Aluminum On Lipid Peroxidation, Superoxide Dismutase, Catalase And Peroxidase Activities In Root Tips Of Soybean (Glycine Max). Physiol. Plant. 83, Pp. 463-468.
  14. Fielding J.L., Hall J.L., 1978. A Biochemical And Cutochemical Study Of Peroxodase Activity In Roots Of Pisum Sativum. 1. A Comparaison Of DAB-Peroxidase And Guaiacol-Peroxidase With Particular Emphasis On The Properties Of Cell Wall Activity. J.Exp. Bot. 29, Pp.  969-981.
  15. Servais , 2004 Alterations Mitochondriales Et Stress Oxydant Pulmonire En Réponse À L’ozone : Effete De L’age Et D’une Supplementation En Omega3. These De Doctorat, Université Claude Bernard Lyon 1.127p
  16. Pierre. Dagnelie, 2013 – Statistique Théorique Et Appliquée (Tome 1) –Tome 1 Statistique Descriptive Et Bases De L’inférence Statistique. Bruxelles, De Boeck, 2013, 517 P.
  17. Arora A., Sairam R.K., Srivastave G.C, 2002. Oxidative Stress And Antioxidative Systems In Plants. Curr. Sci; 82: 1227–1238.
  18. Kohen R & Nyska A, 2002. Oxidation Of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, And Methods For Their Quantification. Toxicol Pathol 30: 620–650.
  19. Rao D.N., Leblanc F.N, 1966. Effets Du Dioxyde De Soufre Sur Le Lichen Algue, Avec Une Référence Particulière À La Chlorophylle Bryologist, 69, Pp 69-75.
  20. David J.C Et Grongnet J.F, 2001. Les Protéines De Stress. INRA Prod. Anim., 14(1): 29-40.
  21. Nzengue Y, 2008. Comparaison Des Mécanismes De Toxicité Redox Du Cadmium , Du Cuivre Et Du Zinc : Place Des Métallo Thionines  Et De P53 .  Thèse De Doctorat, Université  Joseph Fourier – Grenoble 1, France .299 Pages.
  22. Chance B., Sies H., Boveris A, 1979. Hyperoxide Metabolism In Mammalian Organs. Physiological Review; 59: 527-605.
  23. Franco A.A., Odom R.S., Rando T.A, 1999. Regulation Of Antioxidant Enzyme Gene Expression In Response To Oxidative Stress And During Differentiation Of Mouse Skeletal Muscle. Free Radic Biol Med, 27 (9-10), 1122-1132.
  24. Ramel F, 2009. Implication Des Sucres Solubles Dans Les Réponses Aux Stress Xénobiotique Et Oxydatif Chez Arabidopsis Thaliana. Thèse De Doctorat, Université De Rennes 1, France. 231 Pages.
  25. Khosravinejad F., Heydari R., Farboodnia T, 2008. Antioxidant Responses Of Two Barley Varieties To Saline Stress. Res. J. Biol Sci. 3: 486-490.
  26. Scheidegger C & Schroeter B, 1995. Effects Of Ozone Fumigation On Epiphytic Macrolichens: Ultrastructure, CO2 Gas Exchange And Chlorophyll fluorescence. Environ Pollut 88: 345–354.
  27. Wetmore C.M., 1985. Lichens And Air Quality In Isle Royal NP: Final Report. NPS Contract CX 0001-2-0034. Pp. 1-40.
  28. Das K., U. Dey R., Bhaumik J.K., Datta And N.K. Mondal, 2011. A Comparative Study Of Lichen Biochemistry And Air Pollution Status Of Urban, Semi Urban And Industrial Area Of Hooghly And Burdwan District, West Bengal. Journal Of Stress Physiology & Biochemistry, Vol. 7 No. 4 2011, Pp. 311-323

Publication Details

Published in : Volume 2 | Issue 1 | January-February 2016
Date of Publication : 2016-02-25
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 21-27
Manuscript Number : IJSRST16213
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Monia Serradj Ali Ahmed, Zineeddine Boumedris, Ali Tahar, Mohamed Reda Djebar, " Impact of an atmosphere rich in sulfur dioxide on a foliose lichen species Flavoparmelia caperata (L) Hale from the El Kala National Park (northeast Algerian)", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 1, pp.21-27 , January-February-2016.
Journal URL : https://ijsrst.com/IJSRST16213
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