Home > Archives > IJSRST1622114 IJSRST-Library

Peroxidase Isoenzyme Profiles as Indicators of Sensitivity in SO2-fumigated Crop Plants

Authors(1) :-Aprajita Chauhan

A study was made on the isoenzyme patterns of the anti-oxidative enzyme peroxidase in three crop species viz., Solanum esculentum (tomato), Vigna radiata (mung bean ) and Zea mays (maize)  exposed to low levels of SO2 . Exposure to the  phytotoxicant  gas resulted in alteration in isozyme profiles. The intensity and number of Peroxidase(POD) bands increased in S. esculentum and V. radiata. Changes in isoperoxide zymograms are indicative of enhanced  peroxidation  processs in response to pollutant-stress. This method also underlines the value of Polyacrylamide Gel Electrophoresis (PAGE) in sensitivity screening of degrees of SO2- tolerance, prior to the manifestation of visible injury symptoms
Aprajita Chauhan
Information Broking System, Automation segmentation, coordinates broker, privacy preserving, and Attribute-correlation attack
Mung bean, tomato, maize, electrophoresis, peroxidases, isozyme
  1. Chauhan A., 1989a. Effect of Sulphur Dioxide on Plants at Biochemical and Physiological Levels. PhD. Thesis, University of Garhwal, India. P. 297.
  2. Tripathi A.K. and Gautam M. 2007. Biochemical parameters of plants as indicators of air pollution. J.Environ Biol. 28(1): 127-132.
  3. Rai R., Rajput M., Agrawal M. and Agrawal S.B. 2011. Gaseous air pollutants: A review on current and future trends of emissions and impact on agriculture. J.Sci.Res. BHU,Varanasi. 55:77-102.
  4. Singh L.P., Gill S.S., Gill R. and Tuteja N. 2012. Mechanism of sulphur dioxide toxicity and tolerance in crop plants. In: Improving Crop Resistance to Abiotic Stress. Eds. Tuteja N., Tiburci A.F., Gill S.S. and Tuteja R. (pp. 133-163). Wiley VCH Verlag GmBh & KgaA, Berlin.
  5. Arora A., Sairam R.K. and Srivastava G.C. 2002. Oxidative stress and antioxidative systems in plants. Curr. Sci. 82: 1227–1238.
  6. Chauhan A. 1989b. Superoxide dismutase—a bioindicator of plant response to SO2-stress. In : Plants and pollutants in developed and developing countries . Ed Öztürk M.A. (pp 549-568). Ege University, Izmir, Turkey.
  7. Chauhan A. 2015. Peroxidase Activity as an Indicator of SO2- Tolerance in Crop Plants . Int.J.Innov.Res &Dev. 4: 45-50.
  8. Jokinen J., Karjalainen R., Saynatkari T., Hakkinen A. and Markkanen K. 1983.Effects of air pollution on scots pine needles. I. Effects on total peroxidase activity. Silva Fennica 17(3): 279-287.
  9. Chauhan A. 2015d. Effect of SO2 on ascorbic acid content in crop plants—First line of defense against oxidative stress. Int. J. Innov. Res. & Dev. 4(11) : 8-13.
  10. Kim Y., Lim S., Han S., Lee J., Song W., Bang J., Kwon S., Lee H. and Kwak S. 2007. Differential expression of 10 sweet potato peroxidases in response to sulfur dioxide, ozone, and ultraviolet radiation. Plant Physiol. Biochem. 45: 908-914.
  11. Horsman, D.C. and Wellburn, A.R. (1976). Guide to the metabolic and biochemical effects of air pollutants on higher plants. In Mansfield T.A. (Ed.), Effects of air pollutants on plants (pp. 185-199). Cambridge: Cambridge University Press.
  12. Bucher Wallin I. 1976. Zur Beeinflussung des physiologischen Blattalters von Waldbäumen durch Fluor-Immissionen. Mitt.Eidgen.Anst. For. Vers. Schweiz. 52: 101-158.
  13. Keller T., Schwager H. and Yee-Meiler D. 1976. Der Nachweis Winterlich SO2 Immissionen an jungen Fichten: Einvergleich dreier Methoden. Eur. J. Pathol. 6: 244-249.
  14. Varshney S.R.K. and Varshney C.K. 1985. Response of peroxidase to low levels of SO2. Environ.Exp.Bot. 25: 107-114.
  15. Takahama U. , Vekjovic-lovanovic S. and Heber U. 1992. Effects of air pollutant SO2 on leaves: inhibition of sulfite oxidation in the apolast by ascorbate and of apoplastic peroxidase by sulfite. Plant Physiol. 100: 261-266.
  16. Parui S., Mondal A.K. and Mandal, S. 2001. DNA, peroxidase profile, and biochemical composition changes during the treatment in vitro of pollen of Argemone mexicana L. with SO2. Grana 40: 154-158.
  17. Li L. and Yi H. 2012. Effect of sulfur dioxide on ROS production, gene expression and antioxidant enzyme activity in Arabidopsis plants. Plant Physiol. Biochem. 58: 46-53.
  18. Ali Ahmed M.S., Boumedris Z., Tahar A. and Djebar M.R. 2016. Impact of an atmosphere rich in sulfur dioxide on a foliose lichen species Flavoparmelia caperata (L) Hale from the El Kala National Park (Northeast Algerian). Int. J. Innov. Res. Dev. 2(1): 21-27.
  19. Li Z.G., Wu Y.M., Liu Y. and Yu S.W. 1981. Studies on the response and resistance of plants to sulphur dioxide. Effects of SO2 fumigation on peroxidase of wheat leaves. Acta Phytophysiol. Sinica 7: 363-371.
  20. Yannarelli G.G., Gallego S.M. and Tomaro M.L. 2006. Effect of UV-B radiation on the activity and isoforms of enzymes with peroxidase activity in sunflower cotyledons. Environ.Exp. Bot. 56: 174-181.
  21. Moss D.W.1982. Isoenzymes. Chapman and Hall, London and New York.
  22. Gasper T., Panel C., Hagege D. and Greppin H. 1991.Peroxidases in plant growth, differentiation and developmental processes. In Biochemical, Molecular and Physiological Aspects of Plant Peroxidases. J. Lobarzewsky, H. Greppin, C. Penel and T. Gaspar Eds. University M. Curie-Sklodowska, Poland and University of Geneva, Switzerland, p 249-280.
  23. Ianculescu M. and Budu C.E. 2008. Detecton of resistant individuals to SO2 pollution by using peroxidase activity regulated by a glycoprotein in sessile oak seedlings (Quercus petraea (Matt.) Liebl. Forest Science Proc. Rom. Acad. Series B. 1-2: 101-107.
  24. Davis B.J. 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann.N.Y.Acad.Sci. 121: 404-427.
  25. Mitra, R., Jagannath D.R. and Bhatia, C. 1970. Disc electrophoresis of analogous enzymes in Hordeum. Phytochemistry 2: 1843-1850.
  26. Liu E.H. 1975. Substrate specificities of plant peroxidase isozymes. In: Isozymes II. Physiological Function (Ed. C.L. Markert). Academic Press, New York. Pp837-849.
  27. Fhu Z., Xiaofeng Y. and Jingyang Z. 1994. Effects of simulated acid rain on the injury and physiological responses of crops. J. Environ.Sci. 6(1): 87-92.uz
  28. Curtis C.R. and Howell R.K. 1971. Increases in peroxidase isoenzyme activity in bean leaves exposed to low doses of ozone, Phytopathology 61: 1306-1307.
Publication Details
  Published in : Volume 2 | Issue 2 | March-April 2016
  Date of Publication : 2016-04-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 299-303
Manuscript Number : IJSRST1622114
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Aprajita Chauhan, "Peroxidase Isoenzyme Profiles as Indicators of Sensitivity in SO2-fumigated Crop Plants ", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 2, pp.299-303, March-April-2016
URL : http://ijsrst.com/IJSRST1622114