Surveillance of Helminthes and Molecular Phylogeny of Fasciola Gigantica Infecting Goats in Sadat District, Egypt

Authors(2) :-ElKhtam A. O., Khalafalla R. E

Helminthes cause health problems in ruminants as anemia, anorexia, weight loss, diarrhea, decreased production and deaths therefore this study was aimed to identify helminthes of goats, their incidence and genotyping of Fasciola gigantica recovered from Sadat district (Kafr Dawoud, Wadi Elnatron and ElKhatatba abattoirs) Egypt. The study was applied on 790 animals from March 2014 to March 2015. The overall infection rate with helminthes was 23.54% among slaughtered goats at Sadat district, Egypt. Prevalence in different localities was recorded. The overall prevalence rate of trematode helminth was 9.66% and the recovered trematode species and their associated infection rates were Paramphistomum cervi (1.43%), Fasciola hepatica (0.41%), Fasciola gigantica (5.83%), Carmyerius gregarious (1.99%). While the prevalence rate of cestodes was 9% and the recovered species and their correlated infection rates were Moniezia expansa (4.28%), Moniezia benedeni (0.71%) and Avitellina centripunctata (4%). Genotyping of Fasciola gigantica of the current study was done by amplifying 18S ribosomal RNA gene (656 bp of nucleic acids), there are no Single Nucleotides Polymorphisms (SNPs)

Authors and Affiliations

ElKhtam A. O.
Department of Parasitology, Faculty of Veterinary Medicine, University of Sadat City, Egypt
Khalafalla R. E
Department of Parasitology, Faculty of Veterinary Medicine, University of Kafrelsheikh, Kafr El-Sheikh, Egypt

Goat, Helminth, Surveillance, Fasciola Gigantica, Molecular, Phylogeny Egypt

  1. Cawthorne, R.J.C. 1984. Anthelminthics for cattle, sheep, goats, pigs, horses and poultry. MAFF publication lion house, willowbum estate, Alinwick.
  2. Morgan, J. A. and Blair, D. 1995. Nuclear rDNA ITS sequence Variation in the trematode genus Echinostoma: An aid to establishing relationships within the 37-collar spine group. Parasitology, 111: 609-615.
  3. Leon-Regagnon, V.; Brooks, D.R. and de Leon, G.P.P. 1999. Differentiation of Mexican species of Haematoloechus Looss, 1899 (Digenea: Plagiorchiformes): Molecular and morphological evidence. J. Parasitol., 85: 935-94.
  4. Huang, W.Y.; He, B.; Wang, C.R. and Zhu, X.Q. 2004. Characterization of Fasciola species from Mainlad China by ITS-2 ribosomal DNA sequence Vet. Parasitol., 120: 75-83.
  5. Amer, S.; Dar, Y.; Ichikawa, M.; Fukuda, Y.; Tada, C.; Itagaki, T. and Nakai, Y. 2011. Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers. Parasitol. Int., 60: 5-12.
  6. Lotfy, W.M. and Hillyer, G.V. 2003. Fasciola species in Egypt. Experimental Pathology and Parasitology, 6: 9-22.
  7. MAFF, 1986. Manual of Veterinary Parasitological Laboratory Techniques. Ministry of Agriculture, Fisheries and Food. Reference Book No. 418. Her Majesty`s Stationary Office, London, UK.
  8. Soulsby, E.J.L., 1986. Heminths, Arthropods and protozoa of domesticated animals. 7th ed. Baillier, Tidal and Cassel, London.
  9. Sahba, G.H., Arfaa, F., Farahmandian, I., Jalali, H., 1972. Animal fascioliasis in Khuzestan, southwestern Iran. Journal of Parasitology 58, 712–716.
  10. Muller, R., 2002. Worms and Human Diseases. CABI International, Wallingford, Oxon, UK.
  11. El-Gozamy B.R. and Shoukry N.M., 2009. Identification of Egyptian Fasciola species by PCR and restriction endonuclease digestion of the nuclear small subunit ribosomal RNA gene. Journal of Egyptian Society of Parasitology, 39, 429–439
  12. Bowles J, Hope M, Tiu WU, Liu X, McManus DP (1993) Nuclear and mitochondrial genetic markers highly conserved between Chinese and philippine Schistosoma japonicum. Acta Trop (Basel) 55: 217±229
  13. Omar, M.A., Metwally, A.M. and Sultan, K., 2013. Molecular and Phylogenetic status of Fasciola sp., of cattle in Qena, Upper Egypt. Pak. J. Biol. Sci, 16(15): 726-730.
  14. Higgins, D.G. and Sharp, P.M., 1988. CLUSTAL: A package for performing multiple sequence alignment on a microcomputer. Genetics, 73: 237-244.
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., and Kumar S., 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution (In Press).
  16. Mir, M.R.; Chishti, M.Z.; Dar, S.A.; Katoch, R.; Rashid, M.; Ahmad, F. and Tak, H. 2013. Incidence and Histopathological Study of Monieziosis in Goats of Jammu (J&K), India. Cibtech Journal of Zoology, 2 (1): 19-23.
  17. Elkhtam, A.O. 2008. Studies on helminthes infecting ruminants in Menofia governorate. M. V. S., Fac. Vet. Med., Menofia Univ.
  18. Mazyad, S. and El-Nemr, H. 2002. The endoparasites of sheep and goats, and shepherd in North Sinai Governorate, Egypt. Egypt Soc. Parasitol, 32 (1) : 119-126.
  19. Adlard, R.D.; Barker, S.C.; Blair, D. and Cribb, T.H. 1993. Comparison of the second internal transcribed spacer (Ribosomal DNA) from populations and species of fasciolidae (Digenea) .Int. J. Parasitol, 23: 423-425.
  20. Saitou N. and Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  21. Tamura K., Nei M., and Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035.
  22. Dar, Y.; Amer, S.; Mercier, A.; Courtioux, B. and Dreyfuss, G. 2012. Molecular identification of Fasciola spp., (Digenea: Fascioladae) in Egypt. Parasite, 19: 177-182.

Publication Details

Published in : Volume 2 | Issue 4 | July-August 2016
Date of Publication : 2016-08-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 188-192
Manuscript Number : IJSRST162448
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

ElKhtam A. O., Khalafalla R. E, " Surveillance of Helminthes and Molecular Phylogeny of Fasciola Gigantica Infecting Goats in Sadat District, Egypt", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 4, pp.188-192, July-August-2016.
Journal URL :

Article Preview