Isolation and Identification of Bacteria from Different Soil Samples of Telangana and Andhra Pradesh States

Authors(2) :-Arsheen Tabassum, Dr. M. Shailaja Raj

The word agriculture is more related to natural activity and to nature like plant life cycle, ecosystem, green earth, vegetables, fruits, soil, water & natural environment. Plants have a number of relationships with fungi, bacteria, and algae. These are known to deliver a number of benefits including plant nutrition, disease resistance, tolerance to adverse soil and climatic conditions. These techniques have proved to be successful biofertilizers that form a healthy relationship with the roots. Biofertilizers provide eco-friendly organic agro-input and are more cost-effective than chemical fertilizers.

In the present studies various soil samples were collected from actively cultivated lands after a thorough survey of the areas. The places from where these soil samples were collected include Gandipet, Shamirpet areas in Hyderabad, Chevella on the outskirts of Hyderabad and Adilabad district of Telangana State and Kurnool, Kadapa, Gudipadu areas of Andhra Pradesh State. Most of the soils thus obtained were seem to be supporting agriculture actively. These soil samples thus collected were packeted and brought to the laboratory for further microbiological testing. Characterization and microscopic observation of the culture was done and the bacteria identified were Streptomyces sp., Staphylococcus sp., Azotobacter sp., Leptothrix sp., Derxia sp., Bacillus sp., and Sphaerotilus sp. Of the above isolated organisms Azotobacter sp. Streptomyces sp. and Bacillus sp. are familiar to be used as biofertilizers in various crop fields. Derxia sp. is a Gram negative, nitrogen fixing bacteria but not much known as a biofertilizer, which seems to be very interesting to scientist to work on it. Hence it is important to study its role in agriculture.

Authors and Affiliations

Arsheen Tabassum
Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad, Telangana, India
Dr. M. Shailaja Raj
Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad, Telangana, India

Soil samples, Biofertilizers, Bacteria, Crop fields, Farmers.

  1. Vessey, J. K., "Plant growth promoting Rhizobacteriaas bio-fertilizers". Plant Soil ,2003, 255, 571-586
  4. De Freitas, J. R. and Germida, J. J., "Plant growth promoting rhizobacteria for winter Wheat" .Can. J. Microbiol., 1990; 36, 265.
  5. Khosro Mohammadi and Yousef Sohrabi,"Bacterial biofertilizers for sustainable crop Production". ARPN Journal of Agricultural and Biological Science: may 2012; vol.7,no. 5.
  6. Mohd Mazid and Taqi Ahmed Khan. "Future of Bio-fertilizers in Indian Agriculture" International Journal of Agricultural and Food Research , ISSN 1929-0969 , 2014; Vol. 3 , No.3, pp. 10-23.
  7. Tinatin Doolotkeldieva et al, "Effects of Streptomyces Biofertilizer to Soil Fertility and Rhizosphere’s Functional Biodiversity of Agricultural Plants’’. Advances in Microbiology, 2015, 5, 555-571
  8. Biotechnology of Biofertilizers - Page 54 - Google Books Result
  9. Zohreh Mazinani1, Mehdi Aminafshar, Ahmad Asgharzadeh, Mohammad Chamani "Effect of Azotobacter population on physico–chemical characteristics of some soil samples in Iran". Annals of Biological Research, 2012; 3 (7):3120-3125.
  10. Sartaj a. wani et al, "Potential Use of Azotobacter Chroococcum in Crop Production: An Overview, ISSN: 2347-4688, Online ISSN: 2321–9971.
  11. Xie, Cheng-Hui; Yokota, Akira "Phylogenetic analyses of the nitrogen-fixing genus Derxia". The Journal of General and Applied Microbiology, 2004; 50 (3):129– 35.
  12. Roy, A. B.; Sen, Subir A New Species of Derxia". Nature , 1962; "194 (4828): 604–5.
  13. The ProkaryotesA Handbook on the Biology of Bacteria Vol 5 Proteobacteria: Alpha and Beta Subclasses ISBN 3540238611 Springer 2006.
  14. Biotechnology of Biofertilizers - Page 340 - Google Books Result
  15. G.H. Tan and S. A. Nazaruddin, "Isolation, characterization and identification of effective microorganisms from Malaysia biofertilizer products". Director research journal ofAgriculture and Food science (DRJAFS) 2015; vol.3,157- 161.
  16. Pellegrin, Veronique, Stefan Juretschko, Michael Wagner and Gilles Cottenceau. "Morphological and biochemical properties of a Sphaerotilus sp. isolated from paper mill slimes." Appl Environ Microbiol, 1999; vol. 65, no. 1. American Society for Microbiology. (156-162).
  17. BS Saharan and V Nehra. "Plant Growth Promoting Rhizobacteria": A Critical Review Life Sciences and Medicine Research, Volume 2011: LSMR-21.
  18. Azolla Biofertilizer for Sustainable Rice Production
  19. Influence of bio, organic and chemical fertilizers on medicinal pumpkin.
  20. Yoav Bashan, Gina Holguin, and Ran Lifshitz."Isolation and Characterization of Plant Growth-Promoting Rhizobacteria".1993; › Publications.
  21. Annika Durve- Gupta, Bharati Devaguptapu, Vijaya Lobo , Isolation and evaluation of isolated bacteria as potential biofertilizer, International Journal of Applied Research 2016; 2(2): 429-435.

Publication Details

Published in : Volume 2 | Issue 4 | July-August 2016
Date of Publication : 2016-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 23-28
Manuscript Number : IJSRST16246
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Arsheen Tabassum, Dr. M. Shailaja Raj, " Isolation and Identification of Bacteria from Different Soil Samples of Telangana and Andhra Pradesh States", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 4, pp.23-28, July-August-2016.
Journal URL :

Article Preview