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Measurements of Intracellular Free Calcium on Cancer Cell Lines by the Effects of Crude Water-Soluble Extract of Momordica Charantia

Authors(1) :-Dr. Gunasekar Manoharan

The Momordica charantia L., (family: Cucurbitaceae) is a scientific name of the plant and its fruit. It is also known by other names, for instance in the USA it is known as Bitter gourd or balsam pear while its referred to as the African cucumber in many African countries. This study was specifically designed to investigate the cellular mechanisms, whereby crude water soluble extracts of M. charantia can induce cell death measuring the elevation in intracellular free calcium concentrations in four different cancer cell lines 1321N1, Gos-3, U-87 and Sk Mel. 

The results show that incubation of the four cancer cell lines 1321N1, Gos-3,U-87 and Sk Mel with maximum concentrtion of 800 µg of crude water soluble extracts of M. charantia can result in significant (p < 0.05) time-dependent increases in [Ca2+]i in all four cancer cell lines compared  to control (untreated) cells. Maximal increases in [Ca2+]i  was attained after 420 min of incubation. In control (untreated cell lines), [Ca2+]i remained more or less stable in both cell lines after 420 min.  The results also show that the increase in [Ca2+]i in Gos-3 cell line was much more pronounced following incubation with crude water soluble extracts of M. charantia compared to 1321N1 and U-87 cell line. The results show that incubation of the four cancer cell lines with crude water soluble extracts of M. charantia can result in significant (p < 0.05) time-dependent increases in [Ca2+]i in all four cancer cell lines compared  to control (untreated) cells. Maximal increases in [Ca2+]i  was attained after 420 min of incubation. In control (untreated cell lines), [Ca2+]i remained more or less stable in all four cell lines after 420 min.   These results clearly show that crude water soluble extracts of M. charantia  exerting its anti- cancer effect via an insult to the mitochondria resulting in apoptosis, calcium overloading and subsequently, cell death

Dr. Gunasekar Manoharan
Cancer Cells, Crude Water Soluble Extracts of M. Charantia, Intra Cellular Calcium,  Cell Viability
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Publication Details
  Published in : Volume 2 | Issue 5 | September-October 2016
  Date of Publication : 2016-10-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 261-266
Manuscript Number : IJSRST162570
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Dr. Gunasekar Manoharan, "Measurements of Intracellular Free Calcium on Cancer Cell Lines by the Effects of Crude Water-Soluble Extract of Momordica Charantia ", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 5, pp.261-266, September-October-2016
URL : http://ijsrst.com/IJSRST162570