A Pseudomonas Protegens with High Antifungal Activity Protects Apple Fruits Against Botrytis Cinerea Gray Mold

Authors(7) :-Rai Abdelwahab, Bensidhoum Leila, Tabli Nacera, Bouaoud Yousra, Naili Fatma, Cristina Cruz, Elhafid Nabti

Using beneficial bacteria to control plant pathogens has become an ecofriendly alternative to the excessive use of chemicals. In this investigation, a bacterial strain RhiNA, isolated from an agricultural land in northern Algeria (Bejaia), was selected based on its ability to produce antifungal and plant growth promoting (PGP)-metabolites. It was then molecularly identified and screened for its antagonistic activity against B. cinerea, Mucor sp., A. niger and A. flavus. The isolate was also tested for its ability to attenuate gray mold caused by B. cinerea on apple fruits. This strain, identified as Pseudomonas protegens, produced several hydrolytic enzymes, IAA, siderophores, HCN, ammonia and showed potential inhibition of mycelial growth and spore fungal germination (PGI : 66,66,62 and 58% ; SGP : 14.32, 55.10, 28.92 and 10.15% against A. niger, Mucor sp., B. cinerea and A. flavus, respectively). Only 172.823 mm2 of each inoculated area on apple fruits were touched by B. cinerea-gray mold, compared to 529.74 mm2 of rotted zone in absence of the strain. P. protegens-RhiNA shows high efficiency against a virulent fungal strain of B. cinerea. Thus, it could be used as a biocontrol agent for a sustainable agriculture in future.    

Authors and Affiliations

Rai Abdelwahab
Universite de Bejaia, FSNV, Laboratoire de Maitrise des Energies Renouvelables (LMER), Equipe de Biomasse et Environnement, Targa Ouzemmour, 06000 Bejaia, Algerie
Bensidhoum Leila
Universite de Bejaia, FSNV, Laboratoire de Maitrise des Energies Renouvelables (LMER), Equipe de Biomasse et Environnement, Targa Ouzemmour, 06000 Bejaia, Algerie
Tabli Nacera
Universite de Bejaia, FSNV, Laboratoire de Maitrise des Energies Renouvelables (LMER), Equipe de Biomasse et Environnement, Targa Ouzemmour, 06000 Bejaia, Algerie
Bouaoud Yousra
Universite de Bejaia, FSNV, Laboratoire de Mycologie appliquee, Targa Ouzemmour, 06000 Bejaia, Algerie
Naili Fatma
LR Biotechnology and Bio-Geo Resources Valorization, Higher Institute for Biotechnology, University of Manouba, Sidi Thabet Biotechpole,2020 Sidi Thabet, Ariana, Tunisia
Cristina Cruz
Universidade de Lisboa, Faculdade de Ciências, Centro de Ecologia, Evolução e Alterações Ambientais (cE3c), Lisboa, Portugal
Elhafid Nabti
Universite de Bejaia, FSNV, Laboratoire de Maitrise des Energies Renouvelables (LMER), Equipe de Biomasse et Environnement, Targa Ouzemmour, 06000 Bejaia, Algerie

PGPR, Biocontrol, Phytopathogens, IAA, Siderophores, Phosphate Solubilization.

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Publication Details

Published in : Volume 2 | Issue 6 | November-December 2016
Date of Publication : 2016-12-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 227-237
Manuscript Number : IJSRST162612
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Rai Abdelwahab, Bensidhoum Leila, Tabli Nacera, Bouaoud Yousra, Naili Fatma, Cristina Cruz, Elhafid Nabti, " A Pseudomonas Protegens with High Antifungal Activity Protects Apple Fruits Against Botrytis Cinerea Gray Mold", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 6 , pp.227-237, November-December-2016.
Journal URL : http://ijsrst.com/IJSRST162612

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