Isolation of Novel Marine Chitinolytic Actinomycete Streptosporangium Sp. and Production Optimization of Extracellular Chitinase

Authors

  • Jebitta M Shirlin  School of Environmental Toxicology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India
  • J. M. V Kalaiarasi  School of Environmental Toxicology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India

Keywords:

Chitin, Chitinase, Actinomycete, Streptosporangium

Abstract

Chitin is the second most abundant natural polymer and widely distributed as a structural component of crustaceans, insects, and other arthropods, as well as a component of the cell walls of most fungi and some algae. Chitinase (EC 3.2.11.14) enzyme is capable of hydrolyzing insoluble chitin to its oligo and monomeric components found in a variety of organisms including viruses, bacteria, fungi insects, higher plants and animals. In the present study, a rare marine actinomycete strain Streptosporangium sp. was isolated from the marine sediments and screened for its ability to degrade chitin. The effects of media composition and various fermentation conditions were optimized. Maximum chitinase activity was observed in the CCMB medium with 0.1% of colloidal chitin. The optimum pH value was found to be 7 and the temperature range was 350C. Highest chitinase activity was observed on the 14th day of incubation, where the growth of the organism was found to be maximum

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International Journal of Scientific Research in Science and Technology

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Published

2016-12-30

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Volume 2, Issue 6 , November-December-2016

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Research Articles

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How to Cite

[1]
Jebitta M Shirlin, J. M. V Kalaiarasi, " Isolation of Novel Marine Chitinolytic Actinomycete Streptosporangium Sp. and Production Optimization of Extracellular Chitinase, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 2, Issue 6 , pp.311-316, November-December-2016.