Home > Archives > IJSRST162650 IJSRST-Library

Isolation of Novel Marine Chitinolytic Actinomycete Streptosporangium Sp. and Production Optimization of Extracellular Chitinase

Authors(2) :-Jebitta M Shirlin, J. M. V Kalaiarasi

Chitin is the second most abundant natural polymer and widely distributed as a structural component of crustaceans, insects, and other arthropods, as well as a component of the cell walls of most fungi and some algae. Chitinase (EC 3.2.11.14) enzyme is capable of hydrolyzing insoluble chitin to its oligo and monomeric components found in a variety of organisms including viruses, bacteria, fungi insects, higher plants and animals. In the present study, a rare marine actinomycete strain Streptosporangium sp. was isolated from the marine sediments and screened for its ability to degrade chitin. The effects of media composition and various fermentation conditions were optimized. Maximum chitinase activity was observed in the CCMB medium with 0.1% of colloidal chitin. The optimum pH value was found to be 7 and the temperature range was 350C. Highest chitinase activity was observed on the 14th day of incubation, where the growth of the organism was found to be maximum
Jebitta M Shirlin, J. M. V Kalaiarasi
Chitin, Chitinase, Actinomycete, Streptosporangium
  1. El-Tarabily, K. A., Soliman, M.H., Nassar, A.H., Al-Hassani, H.A., Sivasithamparam, K., McKenna, F., and Hardy, G.E.St.J. (2000). Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol., 49: 573–83.
  2. El–Tarabily, K.A. (2003). An endophytic chitinase–producing isolate of Actinoplanes missouriensis, with potential for biological control of root rot of lupin caused by Plectosporium tabacinum. Australian J. Botany, 51: 257–66.
  3. Gohel, V., Singh, A., Vimal, M., Ashwini, P., Chhatpar, H.S. (2006). Bioprospecting and antifungal potential of chitinolytic microorganisms. J. Biotechnol. 5, 54–72.
  4. Goodfellow, M., L. J. Stanton, K. E. Simpson, and D. E. Minnikin. (1990). Numerical and chemical classification of Actinopfunes and some related actinomycetes. Gen. Microbiol. 136:19-36.
  5. Hsu, S. C., and Lockwood , J.L. (1975). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Environ. Microbiol. 29 : 422 – 426.
  6. Jung, W. J., An, K. N., Jin, Y. L., Park, R. D., Lim, K. T., Kim, K. Y., and Tim, T. H. (2003). Biological control of damping- off caused by Rhizoctonia solani using chitinaseproducing Paenibacillus illinoisensis KJA-424. Soil Biology & Biochemistry. 35, 1261-1264.
  7. Kawase, T., Yokokawa, S., Saito, A., Fujii, T., Nikaidou, N., Miyashita, K and Watanabe, T. (2006). Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from coelicolor A3 (2). Biosci Biotech Bioch. 70:988–998
  8. Kemmerling, C., Gurtler, H., Kroppenstedt, R.M., Toalster, R., and Stackebrandt, E. (1993). Evidence for the phylogenetic heterogeneity of the genus Syst. Appl. Microbiol. 16:369- 372.
  9. Kim, H.S., Timmis, K.N. and Golyshin, P.N. (2007). Characterization of a chitinolytic enzyme from Serratia sp. KCK isolated from kimchi juice. Applied Microbiology and Biotechnology. 75, 1275e1283.
  10. Mathivanan, N., Kabilan, V., and Murugesan, K. (1998). Purification, characterization and anti-fungal activity from Fusarium chlamydosporum, a mycoparasite to groundnut rust, Puccinia arachidis. J. Microbiol. 44: 646-651.
  11. Ordentlich, A., Elad, Y. and Chet, I. (1988). The role of chitinase of Serratia mercesens in biocontrol of Sclerotium rolfsii. Journal of Phytopathology. 78, 84-88.
  12. Reguera, G., and Leschine, S. B. (2001). Chitin degradation by cellulolytic anaerobes and facultative aerobes from soils and sediments. FEMS Microbiol Lett. 204: 367-374.
  13. Reissig, J.L., Strominger, J.L and Leloir, L.F. (1955). A modified calorimetric method for the estimation of N-acetyl amino sugars. Biol. Chem. 217, 959-966.
  14. Stakebrandt, E, Kroppenstedt, R.M, Jahnke, D, Kemmerling, C and Gurtler.H. (1994). Transfer of Streptosporangium viridogriseum, Streptosporangium viridogriseum kofuense, and Streptosporangium albidum to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., Respectively, and Emendation of the Genus Streptosporangium. International Journal of Systemic Bacteriology. 44(2): 265-269.
  15. Tronsmo, A. (1991). Biological and integrated controls of Botrtis cinerea on apple with Trichoderma harzianum. Biological Control. 1, 59-62.
  16. Vogelsang, R., and Barz, W. (1993) Purification, characterization and. differential hormonal regulation of a /3-1, 3-glucanase and two chitinases from chickpea (Cicer arietinum ). Planta. 189: 60-69
  17. Xayphakatsaa, K., Tsukiyamaa, T., Inouyeb, K., Okumotoa, Y., Nakazakia, T., Tanisaka, T. (2008). Gene cloning, expression, purification and characterization of rice (Oryza sativa L.) class II chitinase CHT11. Enzyme and Microbial Technology. 43, 19e24.
  18. Yang, C.Y., Ho, Y.C., Pang, J.C., Huang, S.S., and Tschen, J.S. (2009). Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field. Bioresource Technology. 100, 1454e1458.
  19. Ye, X., and Ng, B.T. (2005). A chitinase with antifungal activity from the mung bean. Protein expression and purification. 40: 230-236.
  20. Zhang, H., Huang, X., Fukamizo, T., Muthukrishnan, S., and Kramer, K.J. (2002). Site–directed mutagenesis and functional analysis of an active site tryptophan of insect chitinase. Insect Biochem. Molec., 32: 1477– 88.
Publication Details
  Published in : Volume 2 | Issue 6 | November-December 2016
  Date of Publication : 2016-12-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 311-316
Manuscript Number : IJSRST162650
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Jebitta M Shirlin, J. M. V Kalaiarasi, "Isolation of Novel Marine Chitinolytic Actinomycete Streptosporangium Sp. and Production Optimization of Extracellular Chitinase", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 6 , pp.311-316, November-December-2016
URL : http://ijsrst.com/IJSRST162650