Isolation of Novel Marine Chitinolytic Actinomycete Streptosporangium Sp. and Production Optimization of Extracellular Chitinase

Authors(2) :-Jebitta M Shirlin, J. M. V Kalaiarasi

Chitin is the second most abundant natural polymer and widely distributed as a structural component of crustaceans, insects, and other arthropods, as well as a component of the cell walls of most fungi and some algae. Chitinase (EC enzyme is capable of hydrolyzing insoluble chitin to its oligo and monomeric components found in a variety of organisms including viruses, bacteria, fungi insects, higher plants and animals. In the present study, a rare marine actinomycete strain Streptosporangium sp. was isolated from the marine sediments and screened for its ability to degrade chitin. The effects of media composition and various fermentation conditions were optimized. Maximum chitinase activity was observed in the CCMB medium with 0.1% of colloidal chitin. The optimum pH value was found to be 7 and the temperature range was 350C. Highest chitinase activity was observed on the 14th day of incubation, where the growth of the organism was found to be maximum

Authors and Affiliations

Jebitta M Shirlin
School of Environmental Toxicology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India
J. M. V Kalaiarasi
School of Environmental Toxicology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India

Chitin, Chitinase, Actinomycete, Streptosporangium

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Publication Details

Published in : Volume 2 | Issue 6 | November-December 2016
Date of Publication : 2016-12-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 311-316
Manuscript Number : IJSRST162650
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Jebitta M Shirlin, J. M. V Kalaiarasi, " Isolation of Novel Marine Chitinolytic Actinomycete Streptosporangium Sp. and Production Optimization of Extracellular Chitinase", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 2, Issue 6 , pp.311-316, November-December-2016.
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