A Validated HPTLC Method for the Quantification of ?-Sitosterol In Leaves, Bark of Putranjiva Roxburghii Wall

Authors

  • Kalyani Abhimanyu Kedar  P. E. Society’s Modern College of Pharmacy, Nigdi, Pune, Maharashtra, India
  • Sanjay R. Chaudhari  Rasiklal M. Dhariwal Institute of Pharmaceutical Education and Research, Pune, Maharashtra, India.
  • Avanapu S. Rao  Bhaskar Pharmacy College, Yeknapally, Moinabad (Mandal), R.R. (Dt), Hyderabad, Telangana, India

Keywords:

Putranjiva roxburghii Wall; Euphorbiaceae; B-sitosterol; HPTLC; Method validation.

Abstract

Objective: A simple and sensitive high-performance thin-layer chromatography method was developed and validated for the determination of ?-sitosterol in Putranjiva roxburghii Wall leaf and bark
Methods: Analysis of samples was performed on TLC aluminium precoated plate (60F 254) by using mobile phase toluene: ethyl acetate: formic acid (9:1:0.1v/v/v). TLC plate derivatized with vanillin sulphuric acid reagent. The method was validated using International Council for Harmonization (ICH) guidelines, including linearity, precision, accuracy, and robustness.
Results: A good linearity relationship was found to be with correlation coefficient (r2) value of 0.9951 for ?-sitosterol, from calibration curve it shows presence of 0.16%w/w for ?-sitosterol in leaf extract, 0.07% w/w in bark extract of Putranjiva roxburghii Wall (Family:Euphorbiaceae). Limit of detection and limit of quantitation was found to be 0.04, 0.13 ng spot-1 respectively for ?-sitosterol. The interday and intraday precision was found to be 1.33%, 1.99% (%RSD). Accuracy of the method was performed by recovery studies at three different concentration levels and the average percentage recovery was found to be 98.05% for ?-sitosterol.
Conclusion: The proposed method for the quantitation of ?-sitosterol was found to be simple, specific, accurate and robust in Putranjiva roxburghii Wall. 

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International Journal of Scientific Research in Science and Technology

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Published

2017-12-31

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Volume 3, Issue 10, November-December-2017

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Research Articles

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[1]
Kalyani Abhimanyu Kedar, Sanjay R. Chaudhari, Avanapu S. Rao, " A Validated HPTLC Method for the Quantification of ?-Sitosterol In Leaves, Bark of Putranjiva Roxburghii Wall, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 3, Issue 10, pp.73-78, November-December-2017.