Home > Archives > IJSRST1731026 IJSRST-Library

Translocation of Cyclophosphamide by Using Multi-Walled Carbon Nanotubes Into Mammalian Cancer Cells

Authors(2) :-P. S. Uttekar, V. V. Chopade

The aim of the present work was to prepare cyclophosphamide loaded MWCNTs for target drug delivery of Cyclophosphamide (CP), an Anti-Cancer drug. Experimental designing was done by using the Ultrasonic probe sonicator, PCI Analytics DP-120 under continuous stirring. Translocation of cyclophosphamide was done on functionalized MWCNTs. Functionalization of MWCNTs was done by Acid Purification of pure MWCNTs & conjugation of FA-EDA with oxidized MWCNTs.The loaded MWCNTs were characterized for various parameters including the MTT assay,DSC,SEM,FTIR, Zeta Potential,NMR,Particle Size Analysis, XRD, Stability Study & poly dispersive Index.it is worth that the cyclophosphamide loaded MWCNTs showed better targeting action.
P. S. Uttekar, V. V. Chopade
Multiwall Carbon nanotubes (MWCNTs), cyclophosphamide, Functionalization of MWCNTs, Translocation.
  1. Mehra N.K, and Jain N.K,"Development, characterization and cancer targeting potential of surface engineered carbon nanotubes", (Journal of Drug Targeting, 3 July 2013, 745-758)
  2. Vaibhav Rastogi, Pragya Yadav , Shiv Shankar Bhattacharya, Arun Kumar Mishra, Navneet? Verma and jayanta Kumar Pandit,"Carbon Nanotubes: An Emerging Drug Carrier for Targeting Cancer Cells",( Journal of drug delivery Volume 20141-23)
  3. H.Qui,J.Yang," Structure and Properties of Carbon Nanotubes",(Elsevier Publication,2017,47-66)
  4. Swatantra Kumar Singh Kushwaha,Saurav Ghoshal,Awani Kumar Rai,Satyawan Singh,"Carbon Nanotubes As A Novel Drug Delivery System For Anticancer therapy: A Review"(Brazalian? journal Of Pharmaceutical Sciences,4 Oct,2013,630-642)
  5. Rajashree Hirlekar,ManohYamagar,HarshalGarse, Mohit Vij,Vilasrao kadam, Carbon Nanotubes And It's Application: A Review (2009); 17-27
  6. Neelesh Kumar Mehra and N.K.Jain,"Cancer targeting propensity of folate conjugated surface engineered Multi-Walled Carbon Nanotubes", (Colloids and Surfaces B: Biointerfaces, 27 April 2015)44
  7. Maurizio Prato, Kostas Kostarelos and Alberto Bianco, "Functionalized Carbon Nanotubes in Drug Design and Discovery"(Accounts Of Chemical Research,Jan,2008,60-68)
  8. Mehra N.k , and Jain N.K," Development , Characterization and Cancer Targeting Potential of Surface Engineered CNTs"(Journal Of Drug Targeting ,3 July 2013,745-758)
  9. "Folic Acid". Drugs.com. American Society of Health-System Pharmacists. 1 January 2010. Retrieved 1 September 2016.
  10. Florey Klaus, Abdullah A. Al-Badr, Wozniak Timothy J,"Analytical Profile of Drug Substances", Volume-9, Academic Press An Inprint of Elsevier, 24-28 Oval Road, London 2005.
  11. Monagle, J. J. (1962). "Carbodiimides. III. Conversion of Isocyanates to Carbodiimides. Catalyst Studies". J. Org. Chem. 27 (11): 3851-3855.
  12. Sheehan, John; Cruickshank, Philip; Boshart, Gregory, "A Convenient Synthesis of Water-Soluble Carbodiimides", (1961); J. Org. Chem. 26 (7): 2525.
  13. Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. "Hydrogen Ion Buffers for Biological Research", (1966);? Biochemistry 5 (2): 467-477.
  14. "N-Hydroxysuccinimide" (http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/56480). Sigma-Aldrich. http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/56480. Retrieved 2007-07-03.
  15. Hans-Jurgen Arpe,"Industrielle Organische Chemie-6", Auflage (2007); Seite 245, Wiley VCH.
  16. Nabil Fakhre, Alaadin Naqisshbandi,"Spectrophotometric derivation of Cyclophosphamide", (African Journal of Biotechnology,Nov 2013,6531-6537).
  17. "Indian Pharmacopeia" Edition 2010, Volume-II, Published by Indian Pharmacopeia Commission, Ghaziabad.
  18. Klaus Florey, Jerome I. Bodin, Hans-Georg Leeman,"Analytical Profile of Drug Substances", Volume-9, Academic Press An Inprint of Elsevier, 24-28 Oval Road, London 2005.
  19. Neelesh Kumar Mehra and N.K.Jain,"Cancer targeting propensity of folate conjugated surface engineered Multi-Walled Carbon Nanotubes", (Colloids and Surfaces B: Biointerfaces, 27 April 2015)44
  20. Neelesh Kumar Mehra, and Narendra Kumar Jain,"One platform comparison of Estrone and Folic acid anchored surface engineered MWCNTs for Doxorubicin delivery", (Molecular Pharmaceutics, ACS Publications Washington DC, 17 Dec 2014)43
  21. Sharma Sonam, Neelesh Kumar Mehra, Keerti Jain, and Narendra Kumar Jain, "Effect of functionalization on drug delivery potential of carbon nanotubes" Artificial cells, Nanomedicine, and Biotechnology 44, no. 8 (2016): 1851-1860.
  22. S.K. Smart, A.I. Cassady, G.Q. Lu, D.J. Martin,"The biocompatibility of carbon nanotubes", S.K. Smart et al. / Carbon 44 (2006); 1034-1047
  23. T. Ramanathan, Frank T. Fisher, Rodney S. Ruoff, and L."Catherine Brinson, Apparent Enhanced Solubility of Single-Wall Carbon Nanotubes in a DeuteratedAcidMixture" Research Letters in Nanotechnology Volume 2008; 4 pages
  24. VukovicGoran, AleksandarMarinkovic, Maja Obradovic, Velimir Radmilovic, Miodrag?olic, Radoslav Aleksic, and Petar S. Uskokovic, "Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes" Applied Surface Science 255, no. 18 (2009): 8067-8075.
  25. Joseph Goldstein (2003),"Scanning Electron Microscopy and X-Ray Microanalysis" Springer. ISBN 978-0-306-47292-3. Retrieved 26 May 2012.
  26. Huang H, Yuan Q, Shah J.S, Misra R.D.K,"A new family of folate-decorated and carbon nanotubes-mediated drug delivery system: synthesis and drug delivery response" Adv Drug Deliv. Rev. 2011, 63, 1332-1339.
  27. VukovicGoran, AleksandarMarinkovic, Maja Obradovic, Velimir Radmilovic, Miodrag?olic, Radoslav Aleksic, and Petar S. Uskokovic, "Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes" Applied Surface Science 255, no. 18 (2009): 8067-8075.
  28. Shen M, Wang S.H, Shi X, Chen X, Huang Q, Petersen E.J, Pinto R.A, Baker J.R, Weber W.J,"Polyethyleneimine-Mediated Functionalization of Multiwalled Carbon Nanotubes: Synthesis,Characterization, and In Vitro Toxicity Assay", J. Phys. Chem. C, 2009, 113, 3150-3156.
  29. Zumdahl, Steven S."Chemical Principles", (2009); 6th Ed. Houghton Mifflin Company. p. A23.
  30. "British Pharmacopeia" Edition 2011, Volume-I, Published by British Pharmacopeia Commission, Queen's Road Teddington Middlesex.
  31. Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. "Hydrogen Ion Buffers for Biological Research", (1966);? Biochemistry 5 (2): 467-477.
  32. Son K.H, Hong J.H, Lee J.W,"Carbon nanotubes as cancer therapeutic carriers and mediators" International Journal of Nanomedicine 2016, Vol-11, 5163-5185
  33. Martel, R. et al.? "Ambipolar Electrical Transport in Semiconducting Single-Wall Carbon Nanotubes", (2001); Physical Review Letters 87 (25): 256805.
  34. ManouchehrVossoughi, Shiva Gojgini, A. Kazemi, Iran Alemzadeh and MajidZeinali,"Conjugation of Amphotericin B to Carbon Nanotubes via Amide-Functionalization for Drug Delivery Applications", (Advance online publication: 19 November 2009)
  35. V. Datsyuk, M. Kalyva, K. Papagelis, J. Parthenios, D. Tasis, A. Siokou, I. Kallitsis, C. Galiotis,"Chemical oxidation of multiwalled carbon nanotubes", CARBON 46(2008); 833-840
  36. T. Ramanathan, Frank T. Fisher, Rodney S. Ruoff, and L."Catherine Brinson, Apparent Enhanced Solubility of Single-Wall Carbon Nanotubes in a Deuterated Acid Mixture" Research Letters in Nanotechnology Volume 2008; 4 pages
  37. VukovicGoran, AleksandarMarinkovic, Maja Obradovic, Velimir Radmilovic, Miodrag?olic, Radoslav Aleksic, and Petar S. Uskokovic, "Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes" Applied Surface Science 255, no. 18 (2009): 8067-8075.
  38. Uttekar. P.S, Kulkarni.A.M, Sable.P.N, Chaudhari.P.D,"Surface modifications of carbon nanotubes with Nystatin for drug delivery applications", (IJPER: Vol-50, Issue-3, Jul-Sept 2016).
  39. Xueyan Cao, Lei Tao, Shihui Wen, WenxiuHou, Xiangyang Shi,"Hyaluronic acid-modified multi-walled carbon nanotubes for targeted delivery of Doxorubicin into cancer cells", (Carbohydrate Research, 27 June 2014)48
  40. NeerajLodhi, Neelesh Kumar Mehra, and Narendra Kumar Jain,"Development and characterization of Dexamethasone Mesylate anchored on Multi-Walled Carbon Nanotubes", (Journal of Drug Targeting, 08 Sept 2012, 21(1):67-76)46
  41. Huang H, Yuan Q, Shah J.S, Misra R.D.K,"A new family of folate-decorated and carbon nanotubes-mediated drug delivery system: synthesis and drug delivery response" Adv Drug Deliv. Rev. 2011, 63, 1332-1339.
  42. Sharma Sonam, Neelesh Kumar Mehra, Keerti Jain, and Narendra Kumar Jain, "Effect of functionalization on drug delivery potential of carbon nanotubes" Artificial cells, Nanomedicine, and Biotechnology 44, no. 8 (2016): 1851-1860.
  43. Anbarasan B, S. VigneshBabu, K. Elango, B. Shriya, and S. Ramaprabhu, "pH responsive release of doxorubicin to the cancer cells by functionalized multi-walled carbon nanotubes" Journal of nanoscience and nanotechnology 15, no. 7 (2015): 4799-4805.
  44. ICH Topic Q1A Stability testing Guidelines: stability testing of new drug substances and products, the European agency for the evaluation of medicinal products Human medicines evaluation unit, CPMP/ICH/380/95.
  45. Iijima, Sumio; Ichihashi, Toshinari, "Single-shell carbon nanotubes of 1-nm diameter", (1993); Nature 363 (6430): 603-605
  46. H.Qui, J. Yang, Structure and properties Of Carbon Nanotubes (2017):47-65
  47. Flahaut, E.; Bacsa, R; Peigney, A; Laurent, C "Gram-Scale CCVD Synthesis of Double-Walled Carbon Nanotubes". Chemical Communications (2003); 1442-1443
  48. Rajashree Hirlekar,ManohYamagar,HarshalGarse, Mohit Vij,Vilasrao kadam, Carbon Nanotubes And It's Application: A Review (2009); 17-27
  49. Kinghong Kwok, Wilson K.S. Chiu."Growth of carbon nanotubes by open-air laser-induced chemical vapor deposition" Carbon 43 (2005); 437-446
  50. K. Jurewicz, K. Babe?, R. Pietrzak, S. Delpeux, H. Wachowska,"Capacitance properties of multi-walled carbon nanotubes modified by activation and ammoxidation" Carbon 44 (2006); 2368-2375
  51. Harini Kantamneni,Akhila Gollakota,"carbon Nanotubes Based Systems for targeted drug delivery: A Review"(International Journal of Engineering Research & Technology , Feb,2013,1-9 )
  52. Alberto Bianco, Kostas Kostarelos and Maurizio Prato,"Applications of carbon nanotubes in drug delivery, Current Opinion in Chemical Biology" (2005); 9:674-679)
  53. 9.Wolfgang K. Maser, Ana M. Benito, M. Teresa Martinez, Production of carbon nanotubes: the light approach, Carbon 40 (2002); 1685-1695
  54. S.K. Smart, A.I. Cassady, G.Q. Lu, D.J. Martin, The biocompatibility of carbon nanotubes, Carbon 44 (2006); 1034-1047
  55. Jonathan N. Coleman, Umar Khan, Werner J. Blau, Yurii K. Gun'ko,"Small but strong: A review of the mechanical properties of carbon nanotube-polymer composites", Carbon 44 (2006); 1624-1652 [52]
  56. V. Datsyuka, M. Kalyvaa, K. Papagelisb, J. Partheniosa, D. Tasisb, A. Siokoua, I. Kallitsisa,c, C. Galiotis,"Chemical oxidation of multiwalled carbon nanotubes", CARBON 46 (2008);? 833 -840
Publication Details
  Published in : Volume 3 | Issue 10 | November-December 2017
  Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 121-136
Manuscript Number : IJSRST1731026
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
P. S. Uttekar, V. V. Chopade, "Translocation of Cyclophosphamide by Using Multi-Walled Carbon Nanotubes Into Mammalian Cancer Cells", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 10, pp.121-136, November-December-2017
URL : http://ijsrst.com/IJSRST1731026