Home > Archives > IJSRST173158
Bioremediation of Diesel Contaminated Water Using Indigenous Hydrocarbon Degrading Bacteria
Authors(3) :-Tudararo-Aherobo Laurelta, Iritare Mudiaga, Ogeleka Doris Fovwe
Petroleum-based refined products such as diesel, kerosene, petrol are the major source of energy for industry and daily life. Accidental spills and leakages occur regularly during the re?ning, transport, and storage of petroleum products. Bioremediation of diesel contaminated water was studied using indigenous degrading bacteria isolated from hydrocarbon contaminated soils obtained from automobile mechanic workshops located at Effurun, Delta State. Four (4) bacteria isolates with high diesel biodegradability potential assessed by turbidity measurement were used for the diesel bioremediation study. The bacteria isolates used for the test were identified as, Acetobacter sp, Staphylococcus sp, Marinococcus sp, and Acinetobacter sp. The test microcosms were incubated for two weeks at 28 ± 2oC. Oil & grease concentrations, bacteria count, pH and turbidity were monitored weekly to assess the biodegradation of the diesel. At the end of the test duration, Acetobacter sp. recorded the highest percentage diesel biodegradation (77.57%) for the 5% diesel experimental set up. Similarly, Acinetobacter sp. recorded the highest percentage diesel biodegradation (81.34%) for the 10% experimental set up. Both residual diesel concentrations obtained are below the DPR recommended limit of 10 mg/L for oil & grease in inland waters. Thus, Acetobacter sp. and Acinetobacter sp. could be used to effectively bioaugment the bioremediation process of diesel contaminated waters within a short period of time.
Tudararo-Aherobo Laurelta, Iritare Mudiaga, Ogeleka Doris Fovwe
Bioremediation, Diesel, Polluted Water, Indigenous, Hydrocarbon Degrading Bacteria
- Abioye P.O., Agamuthu P. and Aziz A. R.(2012). Biodegradation of used oil in soil amended with organic wastes. Biotechnol Res Int. DOI: 10.1155/2012/58704.
- Adam, G and Duncan, H.J (1999). Effect of diesel fuel on growth of selected plant species. Environ Geochem Health 21:353–357.
- Agunwamba, J. C., Ezeogu, L. I., and Chukwu, (2000) I: Effects of Nutrient, Crude Oil Pseudomonas Concentrations II: Fungal Growth. International Journal of Environmental Issues, Vol. 2, No. 1 & 2, Pp. 132-142.
- Aleer S, Adetutu, E.M, Makadia, T.H, Patil S and Ball. A. S.(2011;) Harnessing the hydrocarbon-degrading potential of contaminated soils for the bioremediation of waste engine oil. Water Air Soil Poll. 218:121–130.
- American Public Health Association. (2009). Standard Methods for the Examination of Water and Wastewater. Edited by Arnold E. Greenberg, 19th Edition.
- Agency for Toxic Substances and Disease Registry (ATSDR). (1995). Toxicological profile for Polycyclic Aromatic Hydrocarbons (PAHs). Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service.
- American Petroleum Institute (API). (1990). Basic petroleum data book: Petroleum industry statistics. Washington, DC: American Petroleum Institute 10(3): Section VII.
- Bacha, J., Freel, J.,Gibbs A., Gibb, L and Hemighaus G. (2007.) Diesel fuels technical review. Chevron Production Company, San Ramon, CA., USA. http://www.chevronwithtechron.com/products/documents/Diesel_Fuel_Tech_Review.pdf.
- Bhattacharya D, Sarma P.M., Krishnan S, Mishra S, Lal B (2002). Evaluation of genetic diversity among Pseudomonas citronellolis strains isolated from oilysludge–contaminated sites. Appl. Environ. Microbiol. 69(3): 1435-1441.
- Bamforth, S.M., and Singleton, I. (2005). Bioremediation of Polycyclic Aromatic Hydrocarbons: Current Knowledge and Future Directions. J. Chem. Technol. Biotechnol. 80 (7): 723-736.
- Biswal, B.K, Tiwari, S.N and Mukherji, S. (2008). Biodegradation of oil in oily sludges from steel mills. Bio-resource Technol. 2009; 100:1700–1703. DOI: 10.1016/j.biotech..09.037.
- Dorn, P.H. and Salanitro, J., (2000). Temporal ecology assessment of oil contaminated soils before and after bioremediation. Chemosphere, 40: 4, p. 419-426.
- Gallego, J.L.R, Loredo J, Llamas, J.F, Vazquez F and Sánchez, J (2001). Bioremediation of diesel contaminated soils: evaluation of potential in situ techniques by study of bacterial degradation. Biodegradation 12:325–335.
- Ghazali, F.M, Rahman RNZA, Salleh, A.B, Basri, M. (2004) Biodegradation of hydrocarbons in soil by microbial consortium. Int. Biodegrad. 54:61–67. DOI: 10.1016.
- Grant R. J., Muckian, L. M. Clipson, N. J. W and Doyle. E. M. (2007). Microbial community changes during the bioremediation of creosote-contaminated soil. Letters in Applied Microbiology 44:293-300.
- International Agency for Research on Cancer (IARC). (1998). IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans: Re-evaluation of Some Organic Chemicals, Hydrazine and Hydrogen Peroxide. Volume 71. Lyon, France: World Health Organization, International Agency for Research on Cancer.
- Iwamoto, T., M. Nasu, (2001). Current bioremediation practice and perspective. Journal of Bioscience and Bioengineering, 92(1): 1-8.
- Jain, P.K, Gupta, V. K, Pathak H, Lowry M and Jaroli, D. P. (2010) ( Characterization of 2T engine oil degrading indigenous bacteria, isolated from high altitude (Mussoorie), India. World J Microb. Biotechnology.
- Kang,Y,S and Park, W (2010) Protection against diesel oil toxicity by sodium chloride-induced exo-polysaccharides in Acinetobacter sp. strain DR1. J Biosci Bioeng 109:118–123.
- Kvenvolden, K. A, Cooper, C. K (2003) Natural seepage of crude oil into the marine environment. Geo-Mar Lett 23:140–146.
- Lee, J.Y., Roh, J.R. and Kum H.S. (1994). "Metabolic Engineering of Pseudomonas Pulida for the Simultaneous Biodegradation of Benzene, Toluene and P-xylene Mixture". Biotech Bioeng. 43:1146-1152.
- Lee, K., and Levy, E.M., (1991). "Bioremediation: Waxy Crude Oils in D.C., Stranded on Low-Energy Shorelines. Proceedings of the 1991 on Spill Conference". American Petroleum Institute, Washington D.C. PP. 541-547.
- McKee R.H., Schmitt S and Wong Z, (1990a). The reproductive and developmental toxicity of high flash aromatic naphtha. Toxicologist 10:41.
- Mills, M. M., Ridame, C., Davey M, La roche J, and Geider, R.J. (2004). Iron and phosphorus co-limit nitrogen fixation in the eastern tropical North Atlantic. Nature 429: 292–294.
- Mos, L, Cooper, G.A, Serben K, Cameron M and Koop, B. F (2008). Effects of diesel on survival, growth, and gene expression in rainbow trout Fry. Environmental Science Technology 42:2656–2662.
- Muzyka V., S. Bogovski, A. Viitak, and T. Veidebaum. (2002). Alterations of heme metabolism in lymphocytes and metal content in blood plasma as markers of diesel fuels effects on human organism. Science of the Total Environment 286:73-81.
- Nessel, C.S., Freeman J.J., Forgash R.C. and McKee R.H. (1999). The role of dermal irritation in the skin tumor promoting activity of petroleum middle distillates. Toxicol. Sci. 49 (1): 48 -55.
- Payne J.R and G.D. McNabb, Jr.1984. Weathering of petroleum in the marine environment. Marine Technology Society Journal, 18(3): 24-42.
- Sabate, J., Vinas, M., and Solanas, A.M., (2004). "Laboratory-scale Bioremediation Experiments on Hydrocarbon-Contaminated Soils". Int. Biodet. Biod. 54:19-25.
- Sanders, G, Jones, K.C, Hamilton-Taylor J and Dorr, H, (1993). Concentrations and deposition fluxes of polynuclear aromatic hydrocarbons and heavy metals in the dated sediments of a rural English lake. Environ Toxicol Chem 12:1567–1581.
- Sandro, J.B., Magali, C.C., and Denize Dias, C.F., (2005). "Production off CO2, in Crude Oil Bioremediations in Clay Soil". Brazilian Archives of Biology and Technology. Vol. 48 no Spe.
- TTL , Työterveyslaitos. (2011).OVA, Onnettomuuden vaaraa aiheuttavat aineet, Accident hazardous substances safety instructions (diesel 1202), http://www.ttl.fi/ova/yklista.html.
- Van Hamme JD, Singh A, Ward OP: Recent advances in petroleum microbiology. Microbiol Mol Biol Rev. 2003, 67: 503-549. 10.1128/MMBR.67.4.503-549.2003.
- Vasudevan; DM Vasudevan; S Sreekumari; Vaidyanathan Kannan (2010). Textbook of Biochemistry for Medical Students (6th ed.). Jaypee Medical Publishers. ISBN 9350250160.
- Wang X.B, Chi C.Q, Nie Y, Tang Y, Wu G, Wu X.L (2011). Degradation of Petroleum hydrocarbons (C6-C40) and Crude oil by a novel Dietzia strain. Bioresour Technol 102: 7755 – 7761.
- Xu Y and Lu M (2010). Bioremediation of crude oil-contaminated soil: comparison of different bio-stimulation.
- Yu S.H, Ke L., Wong Y.S, Tam N.F.Y. (2005). Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a consortium enriched from mangrove sediments. Environment International. 31:149–154.
- Zananoli, G., Toro, S. Di, Varese, G.C., Bertolotto, A and Fava, F. (2010). Characterization of two diesel fuel degrading microbial consortia enriched from a non-acclimated, complex source of microorganisms. Microbial Cell factories. 9:10.
- Zhu, X.; Venosa, A.D.; Suidan, M.T.; Lee, K. (2001) Guidelines for the Bioremediation of Marine Shorelines and Freshwater Wetlands; U.S. Environmental Protection Agency, Cincinnati, OH.
Published in : Volume 3 | Issue 1 | January-February 2017
Date of Publication : 2017-02-28
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 352-360
Manuscript Number : IJSRST173158
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Tudararo-Aherobo Laurelta, Iritare Mudiaga, Ogeleka Doris Fovwe, "Bioremediation of Diesel Contaminated Water Using Indigenous Hydrocarbon Degrading Bacteria", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 1, pp.352-360, January-February-2017
URL : http://ijsrst.com/IJSRST173158