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Production, Purification and Applications of L-asparaginases

Authors(5) :-Sikander Ali, Ayesha Akram, Arfa Jabbar, Rabia Riaz, Unaiza Sarwar

L-Asparaginase is found in many living organisms including animals, plants and microorganisms. Owing to the difficult extraction procedures, microorganisms are preferred for its industrial scale production. Commonly L-asparaginase is present in three dimensional tetrameric structure but other forms also exist depending on its different sources. Its biochemicsal properties are also dependent on the organism from which it is obtained. L-asparaginase acts on amino acid L-asparagine in two main steps, first an intermediate is formed and then it is attacked by a water molecule and is hydrolyzed into ammonia and aspartic acid. Deficiency of L-asparagine causes inhibition of nucleic acid synthesis in malignant cells which results in cell death. L-Asparaginase can be produced by both solid state and submerged fermentation, but the SSF is more economical and eco-friendly. Enzyme purification is done either by protein precipitation, dialysis, and chromatography or liquid-liquid extraction procedures. L-Asparaginase is involved in treating acute lymphoblastic leukemia (ALL). It is also used essentially in food industry, biosensing and amino acid metabolism.
Sikander Ali, Ayesha Akram, Arfa Jabbar, Rabia Riaz, Unaiza Sarwar
ALL, SSF, SmF, Biosensors, Antineoplastic
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Publication Details
  Published in : Volume 3 | Issue 4 | May-June 2017
  Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 226-235
Manuscript Number : IJSRST173416
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Sikander Ali, Ayesha Akram, Arfa Jabbar, Rabia Riaz, Unaiza Sarwar, "Production, Purification and Applications of L-asparaginases", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 4, pp.226-235, May-June-2017
URL : http://ijsrst.com/IJSRST173416