Production, Purification and Applications of L-asparaginases

Authors

  • Sikander Ali  Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan
  • Ayesha Akram  Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan
  • Arfa Jabbar  Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan
  • Rabia Riaz  Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan
  • Unaiza Sarwar  Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan

Keywords:

ALL, SSF, SmF, Biosensors, Antineoplastic

Abstract

L-Asparaginase is found in many living organisms including animals, plants and microorganisms. Owing to the difficult extraction procedures, microorganisms are preferred for its industrial scale production. Commonly L-asparaginase is present in three dimensional tetrameric structure but other forms also exist depending on its different sources. Its biochemicsal properties are also dependent on the organism from which it is obtained. L-asparaginase acts on amino acid L-asparagine in two main steps, first an intermediate is formed and then it is attacked by a water molecule and is hydrolyzed into ammonia and aspartic acid. Deficiency of L-asparagine causes inhibition of nucleic acid synthesis in malignant cells which results in cell death. L-Asparaginase can be produced by both solid state and submerged fermentation, but the SSF is more economical and eco-friendly. Enzyme purification is done either by protein precipitation, dialysis, and chromatography or liquid-liquid extraction procedures. L-Asparaginase is involved in treating acute lymphoblastic leukemia (ALL). It is also used essentially in food industry, biosensing and amino acid metabolism.

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International Journal of Scientific Research in Science and Technology

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Published

2017-06-30

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Volume 3, Issue 4, May-June-2017

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Research Articles

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[1]
Sikander Ali, Ayesha Akram, Arfa Jabbar, Rabia Riaz, Unaiza Sarwar, " Production, Purification and Applications of L-asparaginases, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 3, Issue 4, pp.226-235, May-June-2017.