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Production, Purification and Advance Applications of L- Asparaginase (Review)

Authors(4) :-Sadia Sanawer, Sikander Ali, Tahira Mohsin, Ammara Nasir

L-Asparaginase, an enzyme (E.C.3.5.1.1) that is responsible for breakdown of L-asparagine into aspartic acid and ammonia, has significant applications in the therapeutics and food technology. Presently L-Asparaginase is mostly produced by using microbial sources including bacteria, fungi and actinomycetes. In therapeutics L-Asparaginase is used as antileukemic agent to treat acute lymphoblastic leukemia (ALL), a malignancy mostly present in children. In food industry this enzyme is used to diminish the quantity of acrylamide that is present in fried and baked starchy foods and carcinogenic in nature. L-Asparaginase done deamination of asparagine thus reduced acrylamide concentration. Another application is its use in biosensors. Both at industrial scale and at laboratory level production of L-Asparaginase can be done by submerged fermentation (SmF) or by solid-state fermentation (SSF). Comparison of both methods revealed that SSF is preferable over SmF because it is inexpensive, environment-friendly and it provides high yield of L-Asparaginase enzyme. SSF method consumes both industrial and agricultural wastes as their substrate. The contamination is significantly decreased in SSF because of presence of low moisture content. Purification of enzyme can be done by using different techniques i.e. precipitation of sample, dialysis and various chromatographic techniques are used in different combinations. A wise combination of steps increase the purification and yield
Sadia Sanawer, Sikander Ali, Tahira Mohsin, Ammara Nasir
E.C.3.5.1.1, ALL, SSF, L-Asparaginase enzyme, pH
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Publication Details
  Published in : Volume 3 | Issue 4 | May-June 2017
  Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 341-351
Manuscript Number : IJSRST173473
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Sadia Sanawer, Sikander Ali, Tahira Mohsin, Ammara Nasir, "Production, Purification and Advance Applications of L- Asparaginase (Review) ", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 4, pp.341-351 , May-June-2017.
Journal URL : http://ijsrst.com/IJSRST173473

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