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Effects of Rosemary and Thyme Extracts on Acrylamide Formation in Fried Beef

Authors(3) :-Fatemeh Gholami, Alireza Rahman, Toktam Mostaghim

This study examined the effects of rosemary and thyme extracts on acrylamide formation in fried beef. Six samples including T0 (the control sample), T1 (1% thyme and 1% rosemary), T2 (1% thyme), T3 (2% thyme), T4 (1% rosemary), and T5 (2% rosemary) were prepared. The samples were deep fried at 200 °C for 10 minutes. Physicochemical properties (pH and moisture content), oxidative indexes (peroxide value, anisidine value, and total oxidation index), acrylamide level, and sensory properties (flavor, texture, color, aroma, and overall acceptance) of the samples were investigated after frying. A completely randomized factorial design was adopted and all analyses were conducted using SPSS 16.0. According to the results, the addition of rosemary and thyme extracts to beef samples decreased acryl amide formation. The lowest levels of acrylamide were observed in T0 and T1 samples, respectively. The extracts had no significant effects on pH and anisidine values. Increasing the levels of extracts in the samples significantly reduced the peroxide value and total oxidation index, but increased the moisture content. The sensory results indicated that addition of rosemary and thyme extracts to the beef samples did not lead to any significant effects on flavor, texture, color, and aroma. Moreover, T1 and T5 samples had the highest and lowest scores, respectively. In conclusion, both rosemary and thyme extracts could significantly decrease the acrylamide level generated in fried beef. Moreover, 2% rosemary and a combination of 1% thyme and 1% rosemary had the strongest effects on reducing acrylamide and oxidative indexes. However, the samples containing 2% rosemary did not provide acceptable sensory indices. Therefore, T1 can be introduced as the best sample.
Fatemeh Gholami, Alireza Rahman, Toktam Mostaghim
Acrylamide, Fried Beef, Oxidative Indexes, Rosemary, Thyme
  1. S. Eriksson, Acrylamide in Food Products: Identification, Formation and Analytical Methodology, Doctoral thesis, Department of Environmental Chemistry, Stockholm University, Sweden, 2005.
  2. H. Mojska, I. Gielecinska, L. Szponar, M. Oltarzewski, Estimation of the dietary acrylamide exposure of the Polish population, Food and Chemical Toxicology, Volume 48, pp. 2090-2096, 2010.
  3. A. Shipp, G. Lawrence, R. Gentry, T. McDonald, H. Bartow, Acrylamide: Review of toxicity data and dose-response analyses for cancer and non-cancer effects, Critical Reviews in Toxicology, Volume 36, pp. 481-608, 2006.
  4. E. Tareke, P. Rydberg, P. Karlsson, S. Eriksson, M. Trnqvist, Analysis of acrylamide, a carcinogen formed in heated foodstuffs, Journal of Agricultural and Food Chemistry, Volume 50, pp. 4998-5006, 2002.
  5. R. Weisshaar, B. Gutsche, Formation of acrylamide in heated potato products- Model experiments pointing to asparagine as precursor, Deutsche Lebensm Rundschau, Volume 98, pp. 397-400, 2002.
  6. DP. Deryck, Acrylamide in fried, baked or roasted foods may increase risk of cancer in susceptible individuals, International Journal of Food Safety, Volume 9, pp. 14-16, 2007.
  7. VR. Medeiros, F. Mestdagh, B. De Meulenaer, Acrylamide formation in fried potato products - Present and future, a critical review on mitigation strategies, Food Chemistry, Volume 133, pp. 1138-1154, 2012.
  8. A. Mustafa, EA. Kamal, EV. Peterson, R. Andersson, P. man, Effect of extraction pH on acrylamide content in fresh and stored rye crisp bread, Journal of Food Composition and Analysis, Volume 21, pp. 351-355, 2008.
  9. T. Schettgen, H. Drexler, J. Angerer, Acrylamide in the general population-A daily intake estimation, Umweltmedizin in Forschung und Praxis, Volume 7, pp. 331-336, 2002.
  10. C. Gertz, S. Klostermann, Analysis of acrylamide and mechanisms of its formation in deep-fried foods, European Journal of Lipid Science and Technology, Volume 104, pp. 762-771, 2002.
  11. Y. Liu, P. Wang, F. Chen, Y. Yuan, Y. Zhu, H. Yan, X. Hu, Role of plant polyphenols in acrylamide formation and elimination, Food Chemistry, Volume 186, pp. 46-53, 2015.
  12. MR. Al-Sereiti, KM. Abu-Amer, P. Sen, Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials, Indian Journal of Experimental Biology, Volume 37, pp. 124-131, 1999.
  13. Z. Faixov, X. Faix, Biological effects of rosemary (Rosmarinus officinalis L.) essential oil: a review, Folia Veterinaria, Volume 52, pp. 135-139, 2008.
  14. A. Kadri, Z. Zarai, I. Ben Chobba, A. Bekir, N. Gharsallah, M. Damak, R. Gdoura, Chemical constituents and antioxidant properties of Rosmarinus officinalis L. essential oil cultivated from South-Western Tunisia, Journal of Medicinal Plants Research, Volume 5, pp. 5999-6004, 2001.
  15. DG. Machado, MP. Cunha, VB. Neis, GO. Balen, A. Colla, LE. Bettio, A. Oliveira, FL. Pazini, JB. Dalmarco, EL. Simionatto, MG. Pizzolatti, AL. Rodrigues, Antidepressant-like effects of fractions, essential oil, carnosol and betulinic acid isolated from Rosmarinus officinalis L, Food Chemistry, Volume 136, pp. 999-1005, 2013.
  16. U. Justesen, P. Knuthsen, Composition of flavonoids in fresh herbs and calculation of flavonoid intake by use of herbs in traditional Danish dishes, Food Chemistry, Volume 73, pp. 245-250, 2001.
  17. M. Lacroix, W. Smoragiewicz, L. Pazdernik, MI. Kone, K. Krzystyniak, Prevention of lipid radiolysis by natural antioxidants from rosemary (Rosmarinus offi cinalis L.) and thyme (Thymus vulgaris L.), Food Research International, Volume 30, pp. 457-462, 1997.
  18. RS. Farag, AZMA. Badei, FM. Hewedi, GSA. El-Baroty, Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media, JAOCS, Volume 66, pp. 792-799, 1989.
  19. M. Akbarmivehie, H. Baghaei, The effect of addition parsnip herb and its extract on Momtaze hamburger shelf life, European Online Journal of Natural and Social Sciences, Volume 5, pp. 132-146, 2016.
  20. E. Demirok, N. Kolsar c , Effect of green tea extract and microwave pre-cooking on the formation of acrylamide in fried chicken drumsticks and chicken wings, Food Research International, Volume 63, pp. 290-298, 2014.
  21. J. Wyrwisz, A. Ptorak, E. Po awska, M. Pierzcha a, A. Jozwik, M. Zalewska, A. Wierzbicka, The impact of heat treatment methods on the physical properties and cooking yield of selected muscles from Limousine breed cattle, Animal Science Papers and Reports, Volume 30, pp. 339-351, 2012.
  22. V. Gkmen, FJ. Morales, B. Ata, A. Serpen, G. Arribas-Lorenzo, Multiple-stage extraction strategy for the determination of acrylamide in foods, Journal of Food Composition and Analysis, Volume 22, pp. 142-147, 2009.
  23. T. Dzudie, CP. Koueboub, JJ. Essia-Ngangb, CMF. Mbofungb, Lipid sources and essential oils effects on quality and stability of beef patties, Journal of Food Engineering, Volume 65, pp.67-72, 2004.
  24. Standard and industrial research institute of Iran. Meat and meat products- determining moisture by reference method-test method. First review. No 745, 2003.
  25. EG. Bligh, WJ. Dver, A rapid method of total lipid extraction and purification, Canadian Journal of Biochemistry and Physiology, Volume 37, pp.911-917, 1959.
  26. AOCS International, Official Methods and Recommended Practice of the American Oil Chemists Society, 5th Edition, AOCS Press, Champaign, 1998.
  27. Y. Zhang, L. Yang, Y. Zu, X. Chen, F. Wang, F. Liu, Oxidative stability of sunflower oil supplemented with carnosic acid compared with synthetic antioxidants during accelerated storage, Food Chemistry, Volume 118, pp. 656-662, 2010.
  28. S. Urbancic, MH. Kolar, D. Dimitrijevic, L. Demsar, R. Vidrih, Stabilization of sunflower oil and reduction of acrylamide formation of potato with rosemary extract during deep-fat frying, LWT - Food Science and Technology, Volume 57, pp. 671-678, 2014.
  29. A. Napolitano, FJ. Morales, R. Sacchi, V. Fogliano, Relationship between virgin olive oil phenolic compounds and acrylamide formation in fried crisps, Journal of Agricultural and Food Chemistry, Volume 56, pp. 2034-2040, 2008.
  30. B. Behnam, J. Aliakbarlou, Antioxidant effects of Zataria multiflora and Mentha longifolia essential oils on chicken meat stored at 4 C, Journal of Food Industry Research, Volume 23, pp. 533-543, 2013. [In Persian]
  31. A. Hozhabri, M. Ganjkhanlou, A. Zali, A. Emami, A. Akbari Afjani Effect of fish oil and thyme essence on meat quality and meat oxidative stability of Mahabadi kids, Journal of Animal Science Researches, Volume 23, pp. 72-81, 2013. [In Persian]
  32. AD. Woyewoda, SJ. Shaw, PJ. Ke, BG. Burns, Quality indices-lipid related. In Recommended Laboratory Methods for Assessment of Fish Quality, Canadian Technical Report of Fisheries and Aquatic Science, Canada, 1986.
  33. EN. Frankel, SW. Huang, R. Aeschbach, E. Prior, Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol, and rosmarinic acid, in bulk oil and oil-in-water emulsion, Journal of Agricultural and Food Chemistry, Volume 44, pp. 131-135, 1996.
  34. M. Nogala-Kalucka, J. Korczak, M. Dratwia, E. Lampart-Szczapa, A. Siger, M. Buchowski, Changes in antioxidant activity and free radical scavenging potential of rosemary extract and tocopherols in isolated rapeseed oil triacylglycerols during accelerated tests, Food Chemistry, Volume 93, pp. 227-235, 2005.
  35. KI. Sallam, M. Ishioroshi, K. Samejima, Antioxidant and antimicrobial effects of garlic in chicken sausage, Lebensmittel-Wissenschaft Technologie, Volume 37, pp. 849-855, 2004.
  36. K. Pirouti, A. Javadi, F. Nahidi, Effect of thyme (Thymus vulgaria) extract on chemical, microbiological and sensory properties of sausage during storage, Journal of Food Hygiene, Volume 4, pp. 9-21, 2014.
  37. S. Ghafari, Effect of addition red cabbage extracts on oxidative shelf life of hamburger, Islamic Azad University, Shahre Qods Branch, 94 pp, 2015. [In Persian]
  38. DAP. De Abreu, PP. Losada, J. Maroto, JM. Cruz, Evaluation of the effectiveness of a new active packaging film containing natural antioxidants (from barley husks) that retard lipid damage in frozen Atlantic salmon (Salmo salar L.), Food Research International, Volume 43, pp. 1277-1282, 2010.
  39. J. Campo, J. Del, M. Amiot, Antimicrobial effect of rosemary extracts, Journal of Food Protection, Volume 63, pp. 1359-1368, 2000.
  40. Z. Formanek, A. Lynch, K. Galvin, J. Farkas, J. P. Kerry Combined effects of irradiation and the use of natural antioxidants on the shelf-life stability of overwrapped minced beef, Meat Science, Volume 63, pp.433-440, 2003.
  41. GF. Mohamed, EM. Hegazy, M. Abdellatef, Physicochemical properties and mycotoxins contents of Tilapia fish-fillets after solar drying and storage, Global Veterinaria, Volume 7, pp. 138-148, 2011.
  42. A. Banerjee, AK. Verma, AK. Das, V. Rajkumar, AA. Shewalkar, HP. Narkhede, Antioxidant effects of broccoli powder extract in goat meat nuggets , Meat Science, Volume 91, pp. 179-184, 2012.
Publication Details
  Published in : Volume 3 | Issue 4 | May-June 2017
  Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 352-360
Manuscript Number : IJSRST173476
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Fatemeh Gholami, Alireza Rahman, Toktam Mostaghim, "Effects of Rosemary and Thyme Extracts on Acrylamide Formation in Fried Beef", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 4, pp.352-360, May-June-2017.
Journal URL : http://ijsrst.com/IJSRST173476

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