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Biotechnological Perspectives of Microbial Proteases
Authors(4) :-Mimpal Singh, Beenish Muhammad Maalik, Sehrish Muhammad Maalik, Shumaila Arshad
The review briefly elucidates the importance of proteases in living organisms and their wide range of potential applications in the vast areas of research and biotechnology. The important features of the proteases are also exploited in a number of ways and can be used to serve various applications in different industries. Apart from their natural potential of performing various important operations in living tissues, various microbial proteases have potential applications in a number of industries. In cellular environment, proteases are involved in the breakdown of the proteinsí peptide bonds and transform them into smaller fragments of amino acids and peptides which are prerequisite for the differentiation and cellular growth. Proteases have also vast applications in a range of industrial procedures such as food, pharmaceutical, dairy and detergent. Microbial proteases have dominated roles in the industrial sectors. Microbial proteases are exploited for their characteristic feature of hydrolyzing the protein and the rest of the components of wheat and soy beans in the production of soy sauce. The production of proteases can be enhanced via substantiated fermentation methods. The variation in the composition of growth media such as changes in carbon and nitrogen ratio and some other features affecting microbial growth are significant in the evaluating the fermentation procedures. The production of microbial proteases is advantageous because they can be generated rapidly, their production is cost effective and the manipulation of microbial enzymes is quite easy. Proteolytic enzymes can be produced by either submerged fermentation (SmF) or solid state fermentation (SSF). But the latter is far more advantageous because it direct towards many potential benefits for the protease production. The review mainly focuses on the microbial protease production, their functional and structural aspects and the application of these proteolytic enzymes in different industries.
Mimpal Singh, Beenish Muhammad Maalik, Sehrish Muhammad Maalik, Shumaila Arshad
Proteases, Enzyme Production, Microbial Proteases, Industrial Level.
- Abidi, F., J.M. Chobert and T. Haertle. 2011. Purification and biochemical characterization of stable alkaline protease Prot-2 from Botrytis cinerea. Process Biochem., 46: 2301-2310.
- Andrade, V.S., L.A. Sarubbo and K. Fukushima. 2002. Production of extracellular proteases by Mucor circinelloides using D-glucose as carbon source / substrate. Braz. J. Microbiol., 33: 106-110.
- Anitha, T.S. and P. Palanivelu. 2013. Purification and characterization of an extracellular keratinolytic protease from a new isolate of Aspergillus parasiticus. Protein Expr. Purif., 88: 214-220.
- Banerjee, U.C., R.K. Sani, W. Azmi and R. Sani. 1999. Thermostable alkaline protease from Bacillus brevis and its characterisation as a laundry detergent additive. Proc. Biochem., 35: 213-219.
- Biesebeke, R.T, G. Ruijter and Y.S.P. Rahardjo. 2002. Aspergillus oryzae in solid-state and submerged fermentations progress report on a multi-disciplinary project. FEMS Yeast Res., 2: 245-248.
- Brandelli, A., D.J. Daroit and A. Riffel. 2010. Biochemical features of microbial keratinases and their production and applications. Appl. Microbiol. Biotechnol., 85: 1735-1750.
- Chao, Y.P., F.H. Xie, and J. Yang. 2007. Screening for a new Streptomyces strain capable of efficient keratin degradation. J. Environ. Sci., 19: 1125-1128.
- De Souza, P.M. and M.L.A. Bittencourt. 2015. A biotechnology perspective of fungal proteases. Brazil. J. Microbiol., 46(2): 337-346.
- Demain, A.L. and J.L. Adrio. 2008. Contributions of microorganisms to industrial biology. Mol. Biotechnol. 38: 41-55.
- Dhandapani, R. and R. Vijayaragvan. 1994. Production of thermophilic, extracellular alkaline protease by B. stearothermophilus AP-4. World J. Microbiol. Biotechnol., 10: 33-35.
- Geisseler, D. and W.R. Horwath. 2008. Regulation of extracellular protease activity in soil in response to different sources and concentrations of nitrogen and carbon. Soil Biol. Biochem., 40: 3040-3048.
- George, S., V. Raju, M.R.V. Krishnan, T.V. Subramanian and K. Jayaraman. 1995. Production of protease by Bacillus amyloliquefaciens in solid-state fermentation and its application in the unhairing of hides and skins. Process Biochem., 30: 457-46.
- Germano, S., A. Pandey and C.A. Osaku. 2003. Characterization and stability of proteases from Penicillium sp. produced by solid-state fermentation. Enz. Microb. Technol., 32: 246-251.
- Godfrey, T. and S. West. 1996. Introduction to Industrial Enzymology. In: Industrial Enzymology, (2nd ed) Macmillan Press, London, UK. pp 1-8.
- Gupta, R., Q.K. Beg and P. Lorenz. 2002. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biotechnol., 59: 15-32.
- Gupta, R., Q.K. Beg and P. Lorenz. 2002. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biotechnol., 59:15-32.
- Hajji, M., N. Hmidet and K. Jellouli. 2010. Gene cloning and expression of a detergent stable alkaline protease from Aspergillus clavatus ES1. Process Biochem., 45: 1746-1752.
- Haki, G.D. and S.K. Rakshit. 2003. Developments in industrially important thermostable enzymes: a review. Bioresour. Technol., 89: 17-34.
- Hameed, A., M.A. Natt and C.S. Evans. 1996. Production of alkaline protease by a new Bacillus subtilis isolate for use as a bating enzyme in leather treatment. World J. Microbiol. Biotechnol., 12: 289-291.
- Hameed, A., T. Keshavarz and C.S. Evans. 1999. Effect of dissolved oxygen tension and pH on the production of extracellular protease from a new isolate of Bacillus subtilis K2, for use in leather processing. J. Chem. Technol. Biotechnol., 74: 5-8.
- Hmidet, N., A.N. El-Hadj and A. Haddar. 2009. Alkaline proteases and thermostable amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive. Biochem. Eng. J., 47: 71-79.
- Jaouadi, B., S. Ellouz-Chaabouni and M. Rhimi. 2008. Biochemical and molecular characterization of a detergent-stable serine alkaline protease from Bacillus pumilus CBS with high catalytic efficiency. Biochim., 90 : 1291-1305.
- Johnvesly, B. and G.R. Naik. 2001. Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium. Process Biochem., 37: 139-144.
- Kalisz, H.M. 1988. Microbial proteinases. Adv. Biochem. Eng. Biotechnol. 36:1-65.
- Kranthi, V.S., D.M. Rao and P. Jaganmohan. 2012. Production of protease by Aspergillus flavus through solid state fermentation using different oil seed cakes. Int. J. Microbiol. Res., 3: 12-15.
- Kucera, M. 1981. The production of toxic protease by the entomopathogenous fungus Metarhizium anisopliae in submerged culture. J. Invertebr.Pathol. 38: 33-38.
- Kudrya, V.A. and I.A. Simonenko. 1994. Alkaline serine proteinase and lectin isolation from the culture fluid of Bacillus subtilis. Appl. Microbiol. Biotechnol., 41: 505-509.
- Kumar, C.G. and H. Takagi. 1999. Microbial alkaline proteases: From a bioindustrial viewpoint. Biotechnol. Adv., 17: 561-594.
- Kumari, M., A. Sharma and M.V. Jagannadham. 2012. Religiosin B, a milk-clotting serine protease from Ficus religiosa. Food Chem., 131: 1295-1303.
- Laxman, R.S., A.P. Sonawane and S.V. More. 2005. Optimization and scale up of production of alkaline protease from Conidiobolus coronatus. Process Biochem., 40: 3152-3158.
- Li, Q., L. Yi, P. Marek and B.L. Iverson. 2013. Commercial proteases: present and future. FEBS lett., 587: 1155-1163.
- Lopez-Otin, C. and J.S. Bond. 2008. Proteases: multifunctional enzymes in life and disease. J. Biol. Chem., 283: 30433-30437.
- Macchione, M.M., C.W. Merheb and E. Gomes. 2008. Protease production by different thermophilic fungi. Appl Biochem. Biotechnol., 146: 223-230.
- Maheshwari, R., G. Bharadwaj and M.K. Bhat. 2000. Thermophilic fungi: their physiology and enzymes. Microbiol. Mol. Biol. Rev., 64: 461-488.
- Merheb, C.W., H. Cabral and E. Gomes. 2007. Partial characterization of protease from a thermophilic fungus, Thermoascus aurantiacus and its hydrolytic activity on bovine casein. Food. Chem. 104: 127-131.
- Neklyudov, A.D., A.N. Ivankin and A.V. Berdutina. 2000. Properties and uses of protein hydrolysates. Appl. Biochem. Microbiol., 36: 452-459.
- Ogawa, A., A. Yasuhara and T. Tanaka. 1995. Production of Neutral Protease by Membrane-Surface of Aspergillus oryzae IAM2704. J. Ferment. Bioeng. 80: 35-40.
- Ramamurthy, V., C.M. Upadhyay and R.M. Kothari. 1991. An optimized protocol for the preparation and application of acid protease. J. Biotechnol., 21: 187-196.
- Rao, M.B., A.M. Tanksale and M.S. Ghatge. 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol., 62: 597-635.
- Rebeca, B.D., M.T. Pena-Vera and M. Diaz-Castaneda. 1991. Production of fish protein hydrolysates with bacterial proteases; yield and nutritional value. J. Food Sci., 56: 309-314.
- Sabotic, J. and J. Kos. 2012. Microbial and fungal protease inhibitors-current and potential applications. Appl. Microbiol. Biotechnol., 93: 1351-1375.
- Savitha, S., S. Sadhasivam and K. Swaminathan. 2011. Fungal protease: Production, purification and compatibility with laundry detergents and their wash performance. J. Taiwan Inst. Chem. Eng., 42: 298-304.
- Showell, M.S. 1999. Enzymes, detergent. In: Flickinger MC, Drew SW (ed) Encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation, vol 2. Wiley, New York, pp 958-971.
- Singh, J., N. Batra and R.C. Sobti. 2001. Serine alkaline protease from a newly isolated Bacillus sp. SSR1. Proc. Biochem., 36: 781-785.
- Siota , L.F., L. Blasco1, J.L.R. Rama1, J.B. Velazquez, T. Miguel, A.S. Perez and T.G. Villa. 2014. Recent patents on microbial proteases for the dairy industry. Recent Adv. DNA Gene Seq., 8: 44-55.
- Sun, S.Y., and Y. Xu. 2009. Membrane-bound ‘synthetic lipase’ specifically cultured under solid-state fermentation and submerged fermentation by Rhizopuschinensis: a comparative investigation. Bioresour. Technol., 100: 1336-1342.
- Tavano, O.L. 2013. Protein hydrolysis using proteases: An important tool for food biotechnology. J. Mol. Catal. B. Enz., 90: 1-11.
- Tomar, R., R. Kumar and M.V. Jagannadham. 2008. A stable serine protease, wrightin, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: purification and biochemical properties. J. Agric. Food Chem., 56: 1479-1487.
- Varela, H., M.D. Ferrari, L. Belobradjic, A. Vazquez and M.L.? Loperena. 1997. Skin unhairing proteases of Bacillus subtilis: production and partial characterization. Biotechnol. Lett. 19: 755-758.
- Vignardet, C., Y.C. Guillaume and L. Michel. 2001. Comparison of two hard keratinous substrates submitted to the action of a keratinase using an experimental design. Int. J. Pharm. 224: 115-122.
- Vishwanatha, K.S., A.G.? Rao and S.A. Singh. 2010. Acid protease production by solid-state fermentation using Aspergillus oryzae MTCC 5341: optimization of process parameters. J. Ind. Microbiol. Biotechnol., 37: 129-138.
- Vishwanatha, K.S., A.G.A Rao and S.A. Singh. 2010. Production and characterization of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341. Appl. Microbiol. Biotechnol., 85: 1849-1859.
- Vishwanatha, K.S., A.G.A. Rao and S.A. Singh. 2009. Characterisation of acid protease expressed from Aspergillus oryzae MTCC 5341. Food Chem., 114: 402-407.
- Walsh, K.A. and P.E. Wilcox. 1970. In: Perlmann, G.E., Lorand, L. (ed). Methods Enzymol.,pp 31?226.
- Ward, O.P. 1985. Proteolytic enzymes. In: Moo-Young M (ed) Comprehensive biotechnology, the practice of biotechnology: current commodity products, vol 3. Pergamon Press, Oxford, pp 789-818.
- Ward, O.P. 2011. Proteases. In: Moo-Young, M. (ed.), Comprehensive Biotechnology, Waterloo, Canada, pp 571-582.
- Watanabe, K. 2004. Collagenolytic proteases from bacteria. Appl. Microbiol. Biotechnol. 63: 520-526.
- Wu, T.Y., A.W. Mohammada and J.M. Jahim. 2006. Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate. Enz. Microb. Technol., 39: 1223-1229.
- Yang, F. and I. Lin. 1998. Production of acid protease using thin stillage from a rice-spirit distillery by Aspergillus niger. Enz. Microb. Technol., 23: 397-402.
- Zambare, V., S. Nilegaonkar and P. Kanekar. 2011. A novel extracellularprotease from Pseudomona aeruginosa MCM B-327: enzyme production and its partial characterization.? N. Biotechnol., 28:173-181.
Published in : Volume 3 | Issue 7 | September-October 2017
Date of Publication : 2017-10-31
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 377-386
Manuscript Number : IJSRST17362
Publisher : Technoscience Academy
PRINT ISSN : 2395-6011
ONLINE ISSN : 2395-602X
Cite This Article :
Mimpal Singh, Beenish Muhammad Maalik, Sehrish Muhammad Maalik, Shumaila Arshad, "Biotechnological Perspectives of Microbial Proteases", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 7, pp.377-386, September-October-2017
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