Anti-Ulcerogenic Effect of Some Indian Medicinal Plants on Mucosal Lesion in Rats

Authors

  • Lakshmipriya.G  Department of Zoology, Auxilium college, Vellore, Tamil Nadu, India
  • Dr.Mary Josephine Rani.A  Department of Zoology, Auxilium college, Vellore, Tamil Nadu, India

Keywords:

Antiulcer, gastric lesion, Lantana camara, Annona muricata, Kalanchoe pinnata, free acidity, total acidity, ulcer index, gastric juice.

Abstract

The antiulcer activity of the poly herbal formulation (composed of the leaf extracts from Lantana camara, Annona muricata, Kalanchoe pinnata) was evaluated in necrotizing agents induced ulcer model in rats. The extract at dose of 1000mg/kg produced significant inhibition of gastric lesion induced by above mentioned method. The extract reduced ulcerative lesion, gastric volume, free and total acidity and pH of gastric juice in the model. The result obtained suggesting that extract possesses significant anti-ulcer activity.

References

  1. Alassane Wele, Yanjun Zhang, Christelle Caux, Jean-Paul Brouard,Jean-Louis Pousset, Bernard Bodo, Annomuricatin C.,?? “A novel cyclohexapeptide from the seeds of Annona muricata”, C R Chimie, 2004; 7: 981-988.
  2. Al-Yahya MA, Rafatullah S, Mossa JS, Ageel AM, Al-Said MS, Tariq M. Gastric antisecretory, antiulcer and cytoprotective properties of ethanol extract of Alpinia galanga willd in rats. Phytother Res 1990;4:112-4.
  3. Anon., “ The useful plants of India”, Publications & Information Directorate, CSIR, New Delhi, India, 1986.
  4. Apak R, Guclu K, Demirata B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 2007; 12(7): 1496-1547.
  5. Arakawa T, Nakamura A, Fukuda T, Higuchi K, Nakamura H and Kobayashi K? In vitro adaptive cytoprotection in gastric cells isolated from rat stomach. J Clin Gastroenterol 12 (Suppl 1): 1990; S32-S38.
  6. Berenguer, B., Sanchez, L.M., Qulilez, A., Lopez barreiro, Galvez, J., Martin, M.J. Protective and antioxidant effects of Rhizophora mangle L. against NSAID-induced gastric lesions. J Ethnopharmacol? 2005; Volume 103, Pages 104-200.
  7. Borelli, F., Izzo, A.A. The plant kingdom as a source of anti-ulcer remedies. Phytotherapy Research., 2000; Volume 14, pages 581-91.
  8. Cao H, Wang M, Jia J, Wang Q, Cheng M., “Comparison of the effects of pantoprazole enantiomers on gastric mucosal lesions and gastric epithelial cells in rats”, Health Sci, 2004; 50:1-8.
  9. Christophe Gleye, Alain Laurens,Reynald Hocquemiller, Olivier Laprevote, Laurent Serani and Andre Cave, Cohibins A and B., “Acetogenins from roots of Annona muricata”, Phytochemistry, 1997; 44( 8): pg1541 -1545.
  10. Cullen D J, Hawkey G M, Greenwood D C: Peptic ulcer bleeding in the elderly: relative roles of Helicobacter pylori and non-steroidal anti-inflammatory drugs. Gut 1997; 41 (4): 459-62.
  11. Dhuley JN. Protective effect of Rhinax, a herbal formation against physical and chemical factors induced gastric and duodenal ulcers in rats. Indian J Pharmacol 1999; 31:128-32.
  12. Falcao HS, Mariath IR, Diniz MFFM, Batista LM, Barbosa-Filho JM. Plants of the American continent with antiulcer activity. Phytomed 2008; 15: 132-46.
  13. Falk GW., Cecil Essentials of Medicine. 5th Edn., W.B.Saunders Company, Edinburgh. 2001; 334-343.
  14. Ghani A. “Monographs of the recorded medicinal plants”,? Medicinal Plants of Bangladesh. 2nd ed. Asiatic Society of Bangladesh, 2003; p. 271-272.
  15. Glavin GB.? Szabo S., Experimental Gastric Mucosal Injury Laboratory Models Reveal Mechanisms of Pathogenesis and New Therapeutic Strategies. FASEB J. 1992; 6: 825-831.
  16. Glavin GB, Par? WP, Sandbak T, Bakke HK, Murison R. Restraint stress in biomedical research: an update. Neurosci Biobehav Rev 1994;18:223-49.
  17. Goulart YC, Sela VR, Obici S, Vanessa J, Martins C, Otobone F et al. Evaluation of gastric anti-ulcer activity in a hydroethanolic extract from Kielmeyera coriacea. Braz Arch Biol Techn 2005; 48(1):211-16.
  18. Goyal R. K.? Elements of Pharmacology, B.S. Shah Prakashan, NewDelhi, India, 17th edition, 2008.
  19. Guth PH, Paulsen G and Nagata H.? Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rat: Effect of prostaglandin cytoprotection. Gastroenterology 87: 1984; 1083-1090.
  20. Hajrezaie M, Golbabapour S, Hassandarvish P, Gwaram NS, Hadi AH, Ali HM, et al., “Acute toxicity and gastroprotection studies of a new Schiff base derived copper(II) complex against ethanol induced acute gastric lesions in rats”, PLos One, 2012; doi: 10.1371/journal.pone.0051537.
  21. Kent-Lioyd KC., Debas HT., Peripheral Regulation of Gastric Acid Secretion. Physiology of the Gastrointestinal Tract. Raven Press, New York. 1994; 1126-1185.
  22. Kumar SV, Sankar P and Varatharajan R. Anti-inflammatory activity of roots of Achyranthes aspera. Pharmaceutical Biology. 47 (10); 2009: 973-975.
  23. Magaji RA, Okasha MAM, Abubakar MS, Fatihu MY. Antiulcerogenic and anti-secretory activity of the N-butanol portion of Syzygium aromaticum in rats. Nigerian J Pharm Sci 2007; 6 (2):119-126.
  24. Malairajan . P?? Gopalakrishnan . G? Narasimhan . S Veni K.J. Kavimani S. J. Ethnopharmacol, 2007; 110, 348-351.
  25. Malfertheiner, F. K. Chan, and K. E. McColl, “Peptic ulcer disease,” The Lancet, 2009; vol. 374, no. 9699, pp. 1449-1461.
  26. Masferrer JL, Isakson PC and Seibert K. Cyclooxygenase-2 inhibitors: A new class of anti-inflammatory agents that spare the gastrointestinal tract. Gastroenterol Clin North Am 25: 1996; 363-372.
  27. McKenzie RA, Dunster PJ., “Hearts and flowers: Bryophyllum poisoning of cattle”, Aust. Vet. J, 1986; 63: 222.
  28. Mcquaid KR., Current Medical Diagnosis and Treatment. 1st Edn., Lange Medical Books/McGraw Hill Company, New York. 2002; 616-621.
  29. Miller L C and Tainter M L, Proc. Soc Expt. Biol, Med, 1944; 57-261
  30. Miller TA.? Protective effects of prostaglandins against gastric mucosal damage:Current knowledge and proposed mechanism. Am J Physiol 245: 1983; G601-G623.
  31. Mizuno H, Sakamoto C, Matsuda K, Wada K, Uchida T, Noguchi H, Akamatsu T and Kasuga M.? Induction of cyclooxygenase 2 in gastric mucosal lesions and its inhibition by the specific antagonist delays healing in mice. Gastroenterology 112: 1997; 387-397.
  32. Muzitano MF, Falc?o CAB, Cruz EA, Bergonzi MC, Bilia AR, Vincieri FF, et al., “Oral metabolism and efficacy of Kalanchoe pinnata flavonoids in a murine model of cutaneous leishmaniasis”, Planta Med, 2009; 75: 307-11.
  33. Nayak BS, Marshall JR, Isitor G., “Wound healing potential of ethanolic extract of Kalanchoe pinnata Lam. Leaf-A preliminary study”,? Indian J. Experim. Biol, 2010; 48: 572-576.
  34. Nwafor, P.A., Effrain, K.D., Jack, T.W. Gastroprotective effects of aqueous extract of Khaya senegalensis bark on indomethacin-induced ulceration in rats. West African Journal of PharmacologyandDrugresearch, 1996; volume12, Pages 46-50.
  35. O'Byrne DJ, Devaraj S, Grundy SM, Jialal I. Comparison of the antioxidant effects of Concord grape juice flavonoids alpha-tocopherol on markers of oxidative stress in healthy adults. Am J Clin Nutr 2002; 76(6):1367-1374.
  36. Okwu DE, Nnamdi FU., “Two novel flavonoids from Bryophyllum pinnatum and their antimicrobial Activity”, J. Chem. Pharm. Res, 2011; 3(2):1-10.
  37. Ozsoy N, Candoken E, Akev N. Implications for degenerative disorders: antioxidative activity, total phenols, flavonoids, ascorbic acid, beta-carotene and beta-tocopherol in? Aloe vera. Oxid Med Cell Longev 2009; 2(2): 99-106.
  38. Rafatullah S, Tariq M, Al-Yahya MA, Mossa JS, Ageel AM. Evaluation of turmeric (Curcuma longa) for gastric and duodenal anti-ulcer activity in rats. J Ehthnopharmacol 1990;29:25-34.
  39. Rafatullah S, Galal AM, Al-Yahya MA, Al-Said MS. Gastric antiulcer and cytoprotective effects of Aframomum melegueta in rats. Int J Pharmaco 1995;33:311-6.
  40. Rajkumar V et al. Evaluation of cytotoxic potential of Acorus calamus rhizome. Ethnobotanical? Leaflets. 13 (6); 2009: 832-839.
  41. Repetto MG, Llesuy SF. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res 2002; 35: 523-34.
  42. Rezaeizadeh A, Zuki ABZ, Abdollahi M. Determination of antioxidant activity in methanolic and chloroformic extracts of Momordica Charantia. Afr J Biotech.,? 2011;?? 10 (24): 4932-4940.??????
  43. Robert A.? Cytoprotection by prostaglandins. Gastroenterology 77: 1979; 761-767.
  44. Robert A, Nazamis JE, Lancaster C, Davis JP, Field SO and Hanchar J .? Mild irritants prevent gastric necrosis through “adaptive cytoprotection” mediated by prostaglandins. Am J Physiol 245: 1983; G113-G121.
  45. Sabu MC and Kuttan R. Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property. Journal of Ethnopharmacology. 81 (2); 2002; 155-160.
  46. Sachin, S. S. and Archana, R. J.? Antiulcer activity of methanol extract of Erythrina indica Lam. leaves in experimental animals. Pharmacognosy Research., 2009 1 (6): 396-401.
  47. Sastri BN.,? “The wealth of India”, CSIR New Delhi, India. 1962.
  48. Sawaoka H, Tsuji S, Tsujii M, Gunawan ES, Nakama A, Takei Y, Nagano K, Matsui H, Kawano S and HoriM.? Expression of the cyclooxygenase-2 gene in gastric epithelium. J Clin Gastroenterol 25 (Suppl 1): 1997; S105-S110.
  49. Sidhartha PA, Chandhuri KW., “Anti-inflammatory action of Bryophyllum pinnatum leaf extract”, Fitoterapia, 1990; 41: 527-533.
  50. Shetty BV, Arjuman A, Jorapur A, Samanth R, Yadav SK, Valliammai N, et al. Effect of extract of Benincasa hispida on oxitative stress in rats with Indomethacin-induced gastric ulcers. Indian J Physio Pharmcol 2008; 52(2): 178-182.
  51. Sun WH, Tsuji S, Tsujii M, Gunawan ES, Sawaoka H, Kawai N, Iijima H, Kimura A, Kakiuchi Y, Yasumaru Y, Sasaki Y, Kawano S and Hori M.? Cyclooxygenase- 2 inhibitors suppress epithelial cell kinetics and delay gastric wound healing in rats. J Gastroenterol Hepatol 15: 2000; 751-761.
  52. Supratman UT, Fujita K, Akiyama H, Hayashi A, Murakami H, Sakai K, et al., “Anti-tumor Promoting Activity of Bufadienolides from Kalanchoe pinnata and K. daigremontiana butiflora”, Biosci Biotechnol Biochem, 2000; 165: 947-949.
  53. Tepperman BL., Jacobson ED., Circulatory Factors in Gastric Mucosal Defense and Repair. Physiology of the Gastrointestinal Tract. Raven Press, New York. 1994; 1331- 1352.
  54. Thamotharan G, Sekar G, Ganesh T, Saikat sen, Raja Chakraborty, Senthil.kumar N.,? “Antiulcerogenic effect of Lantana camara Leaves on in vivo test models in Rats”, Asian. Jour. Pharm.Clinical. Res, 2010; 3: 57-60.
  55. Torres-Santos ECS, Da Silva AG, Costa APP, Santos APA, Rossi- Bergmann B., “Toxicological analysis and effectiveness of oral Kalanchoe pinnata on a human case of cutaneous leishmaniasis”, Phytother Res, 2003; 17: 801-803.
  56. Tsuji S, Kawano S, Sawaoka H, Takei Y, Kobayashi I, Nagano K, Fusamoto H and Kamada T.? Evidences for involvement of cyclooxygenase-2 in proliferation of two gastrointestinal cancer cell lines. Prostaglandins Leukot Essent Fatty Acids 55: 1996; 179-183.
  57. Valle P. D. L. “Peptic ulcer diseases and related disorders,” in Harrison’s Principles of Internal Medicine, E. Braunwald, A. S. Fauci,D. L.Kasper, S. L. Hauser,D. L. Longo, and J. L. Jameson, Eds., 2005; pp. 1746-1762,McGraw-Hill, New York, NY,USA.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2017-10-31

Issue

Volume 3, Issue 7, September-October-2017

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Lakshmipriya.G, Dr.Mary Josephine Rani.A, " Anti-Ulcerogenic Effect of Some Indian Medicinal Plants on Mucosal Lesion in Rats , International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 3, Issue 7, pp.773-779, September-October-2017.