Anatomical, Morphological, Palynological, Phytochemical And Molecular Profiling of Medicinal Mangrove Avicennia Marina(Forssk.) Vierh. (Avicenniaceae)

Abstract

Avicennia marina, commonly known as grey mangrove or white mangrove, is a species of mangrove tree classified in the plant family Avicenniaceae. Medicinal mangrove plant used to antimicrobial, antioxidant, anticandidal properties. Specimens were examined by morphological, anatomical, palynological, HPTLC and DNA fingerprinting.  In anatomical studies glandular trichomes occur in both  adaxial and abaxial surfaces of the leaf; on the adaxial side, the glandular hairs are sunken dispersed in crypts, and non-glandular hairs distributed in abaxial side;  nectariferous gland present in petiole. Toluene: Ethyl Acetate: Formic acid: Methanol: 7:5:1:0.5 as good solvent system for measuring the establishment of phytochemicals in HPTLC analysis. Itshowed Rf values ranged in between 0.07 to 0.80 in 254 nm and 0.07 to 0.81 in 366 nm. The concatenated rbcL + matK loci were able to adequately discriminate A. marina genera in Avicenniaceae. Our study provides the endorsement of the species resolution among mangroves using plastid genes. The nucleotide sequence was also deposited in NCBI.