Mitrochondrial Cytochrome Oxidize I (mt COI) Diagnostic Technique for the Quick Identification of Biotypes Whitefly, Bemisia Tabaci (Gennadius) on different Crops

Authors(3) :-Swati Tomar, Kamlesh Malik, Seema Sharma

Bemisia tabaci (Genn.) is one of the important pest and affects a board range of agricultural crops in the world having many biotypes. There is evidence that B. tabaci should be considered a cryptic complex species of 11 well-defined groups containing at least 24 morphologically indistinguishable species. Its populations differ biologically with respect to insecticide resistance, virus transmission and host range. Therefore, understanding genetic variation among populations is important for management. In present study, the mtCO I sequence of B. tabaci was analyzed from population sample collected from different host plants from different area, Uttar Pradesh. Results obtained showed that amplification of mtCO I gene fragment using the primers (C1-J-2195 and L2-N-3014) produced B. tabaci specific ~800bp band in all the samples of whitefly. Further Sequencing, homology search by Blast and comparison against the consensus sequences of Dinsdale or Ellango, revealed that these samples belong to group Asia II. It is highly probable that these all biotypes are the same entity, but have been assigned different names either as a consequence of the use of different methods of identification or a failure to broadly consider the available data in sources such as Genebank.

Authors and Affiliations

Swati Tomar
Zoology Department, Meerut College, Meerut, Uttar Pradesh, India
Kamlesh Malik
Plant Protection, Central Potato Research Institute Campus, Modipuram, Meerut, Uttar Pradesh, India
Seema Sharma

Amplification, Biotypes, COI, Bemisia tabaci

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Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 128-133
Manuscript Number : IJSRST173820
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Swati Tomar, Kamlesh Malik, Seema Sharma, " Mitrochondrial Cytochrome Oxidize I (mt COI) Diagnostic Technique for the Quick Identification of Biotypes Whitefly, Bemisia Tabaci (Gennadius) on different Crops", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 8, pp.128-133, November-December-2017.
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