Mitrochondrial Cytochrome Oxidize I (mt COI) Diagnostic Technique for the Quick Identification of Biotypes Whitefly, Bemisia Tabaci (Gennadius) on different Crops

Authors

  • Swati Tomar  Zoology Department, Meerut College, Meerut, Uttar Pradesh, India
  • Kamlesh Malik  Plant Protection, Central Potato Research Institute Campus, Modipuram, Meerut, Uttar Pradesh, India
  • Seema Sharma  

Keywords:

Amplification, Biotypes, COI, Bemisia tabaci

Abstract

Bemisia tabaci (Genn.) is one of the important pest and affects a board range of agricultural crops in the world having many biotypes. There is evidence that B. tabaci should be considered a cryptic complex species of 11 well-defined groups containing at least 24 morphologically indistinguishable species. Its populations differ biologically with respect to insecticide resistance, virus transmission and host range. Therefore, understanding genetic variation among populations is important for management. In present study, the mtCO I sequence of B. tabaci was analyzed from population sample collected from different host plants from different area, Uttar Pradesh. Results obtained showed that amplification of mtCO I gene fragment using the primers (C1-J-2195 and L2-N-3014) produced B. tabaci specific ~800bp band in all the samples of whitefly. Further Sequencing, homology search by Blast and comparison against the consensus sequences of Dinsdale or Ellango, revealed that these samples belong to group Asia II. It is highly probable that these all biotypes are the same entity, but have been assigned different names either as a consequence of the use of different methods of identification or a failure to broadly consider the available data in sources such as Genebank.

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International Journal of Scientific Research in Science and Technology

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Published

2017-12-31

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Volume 3, Issue 8, November-December-2017

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Research Articles

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[1]
Swati Tomar, Kamlesh Malik, Seema Sharma, " Mitrochondrial Cytochrome Oxidize I (mt COI) Diagnostic Technique for the Quick Identification of Biotypes Whitefly, Bemisia Tabaci (Gennadius) on different Crops, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 3, Issue 8, pp.128-133, November-December-2017.