Isolation of Chitinase Producing Streptomyces Sp. M1 for Recycling of Fungal Biomass

Authors(3) :-S. R Sukalkar, T. A. Kadam, H. J. Bhosale

Chitinases are glycosyl hydrolase which catalyze degradation of chitin. Microorganisms produce variety of chitinases that exhibits different substrate specificity and applicability in various fields. In the present study 107 actinomycetes obtained from water and soil samples of various habitats of Maharashtra, were screened for chitin hydrolysis activity on colloidal chitin agar. 30 isolates with prominent chitin hydrolysis ability were subjected for quantitative screening in colloidal chitin broth. 10 isolates with highest chitin hydrolysis used to study effect of fungal, insect, shrimp chitin and crab chitin on chitinase production.  The isolate M1 from Mumbai sea water showed a good chitinase activity with all types of substrates. The highest activity was observed in the presence of insect chitin- 3.7 U/ml followed byfungal chitin- 3.8 U/ml, shrimp chitin- 3.5 U/ml and crab chitin- 3.4 U/ml. Isolate M1 is further identified microscopically and 16S rRNA sequencing as Streptomyces sp.

Authors and Affiliations

S. R Sukalkar
School of Life Sciences, SRTM University, Nanded, Maharashtra, India
T. A. Kadam
School of Life Sciences, SRTM University, Nanded, Maharashtra, India
H. J. Bhosale
School of Life Sciences, SRTM University, Nanded, Maharashtra, India

Chitin, Chitinase, Streptomyces sp, substrate specificity.

  1. Bansode VB, Bajekal SS (2006): Characterization of chitinases from microorganisms isolated?? from Lonar lake. Indian J Biotechnol 2006; 5:357-363.
  2. N Dahiya, R Tewari, G S Hoondal. (2006). Biotechnological aspects of chitinolytic enzymes: a review. Appl Microbial Biotechnol. 71: 773-782.
  3. R S Galante, A G Taranto, M G B Koblitz. (2012). Purification characterization and structural determination of chitinase produced by Moniliophthora perniciosa. An Acad Bras Cienc. 84(2): 469- 486.
  4. M Kaya, V Baublys, E Can, I Satkauskience, B. Bitim. (2014). Comparision of physiochemical properties of chitins isolated from an insect (Meloloatha melolontha) and a Crustacean Sp. (Oniscus asellus), Zoomarph. 133 (3) pP- 285-293.
  5. W Kenchington. (1976). Adaptation of insects peritrophic membranes to form cocoon fabrics. In the insect integument, pp 497-513.
  6. J P N Kolla, V Muvva. (2009), Chitinase Production by Streptomyces sp ANU6277. Braz J Microbiol. 40: 725-733.
  7. U V Mane, A M Deshmukh, (2009). chitin degrading potential of three aqatic actinomycetes and its optimization. Afr j Biotechnol. 8(23) pp: 6617-6620.
  8. K S Matsumoto. (2014). Adv Agric Food biotechnol. 289.
  9. M Mohseni, H Norouzi, J Hamedi, A Roohi. (2013). Screening of antibacterial producing actinomycetes from sediments of caspaian sea. I J Mol Cell Med Spring. 2(2) 65.
  10. N Murthy, B Bleakly. (2012). Simplified method of preparing colloidal chitin used for screening of chitinase producing microorganisms. I J Microbial. 10 (2).
  11. N Nawani, D Prakash, V G Gadekar, B P Kapadnis. (2007). Enhancement of chitinase production from Microbiospora sp V2 by response surface methodology. Microbes: biofactories of the future. 48th Annual Conference, AMI Chennai, India. Pg-22.
  12. P Pattanapipipaisal, R Kamlandharn. (2012). Screening of chitinolytic actinomycetes for biological control of Sclerotium rolfsii stem rot disease of chill. Songklanakarin J Sci Technol. 34 (40), 387-393.
  13. R S Patil V Ghormade, M V Deshpande. (2002). Chitinolytic enzymes: an exploration. Enzyme AndMicrob Technol. 26: 473-483.
  14. W Peters. (1992) peritrophic membranes. In zoophysiology, vol 1, pp. 1-238.
  15. R Ragunathan, B Krishnaveni. (2015). Extraction and characterization of chitin and chitosan from Bionetria CBNR BKRR, synthesis of their Bionanocemposites and study of their application. Indo Gobal J Pharmaceutical sciences. 5(1): 40-52
  16. L I Rojas Avelizapa, M Gomez Ramirez, R Cruz Camarillo. (2001). In: Fermentation Of shrimp waste to produce proteo-chitinolytic enzymes and insecticidal crystals. Atech, Italy: Chitosan In Pharmacy And Chemistry. Pp. 478-487.
  17. I Saadoun, RAL Omari, Z Jaradat, Q Ababneh. (2009) influence of culture conditions of Streotomyces sp (strains 242) on chitinase production. . Polish J Microbial, 58 (4) 339-345.
  18. V Thiagarahan, R Revsthi, K Aparanji, P Sivamani. (2011). Extracellular chitinase production by Streptomyces sp PTK 19 in submerged fermtation and its lytic activity on Fusarium oxysporim PTK2 cell wall. I J Curr Sci.1: 30- 44.
  19. H Tsujibo, T Kubota, M Yammoto
  20. K? Miyamoto, I Yoshihiko. Appl. Environ? Microbiol. (2003). Characterization of chitinase genes from an alkaliphilic actinomycete Nocardiopsis prasina opc-131. 69:? 894 -900.
  21. N Sharma, K P Sharma, R K Gaur, V K Gupata. (2011). Role of chitinase in plant defense. Asian J Biochem. 6(1): 29-37.
  22. J M Shirlin, J M V Kalaorasi. (2016). Isolation of novel marine chitinolytic actinomycetes Streptosporangium sp. and production optimization of extracellular chitinase. I J Scientific Res Sci Technol. 6(2): 311-316.
  23. Y M Stoykov, A. Pavlov, A L Karstanov. (2015). Chitinase Biotechnology: Production purification and application. Eng life Sci. 15: 30-38.
  24. S L Wang, C H Chao, T W Liang, C C Chen. (2009). Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine waste by these enzymes. Mar. biotechnol. 11: 334-344.

Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 399-404
Manuscript Number : IJSRST173899
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

S. R Sukalkar, T. A. Kadam, H. J. Bhosale, " Isolation of Chitinase Producing Streptomyces Sp. M1 for Recycling of Fungal Biomass", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 8, pp.399-404, November-December-2017.
Journal URL : http://ijsrst.com/IJSRST173899

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