Isolation of Chitinase Producing Streptomyces Sp. M1 for Recycling of Fungal Biomass

Authors(3) :-S. R Sukalkar, T. A. Kadam, H. J. Bhosale

Chitinases are glycosyl hydrolase which catalyze degradation of chitin. Microorganisms produce variety of chitinases that exhibits different substrate specificity and applicability in various fields. In the present study 107 actinomycetes obtained from water and soil samples of various habitats of Maharashtra, were screened for chitin hydrolysis activity on colloidal chitin agar. 30 isolates with prominent chitin hydrolysis ability were subjected for quantitative screening in colloidal chitin broth. 10 isolates with highest chitin hydrolysis used to study effect of fungal, insect, shrimp chitin and crab chitin on chitinase production.  The isolate M1 from Mumbai sea water showed a good chitinase activity with all types of substrates. The highest activity was observed in the presence of insect chitin- 3.7 U/ml followed byfungal chitin- 3.8 U/ml, shrimp chitin- 3.5 U/ml and crab chitin- 3.4 U/ml. Isolate M1 is further identified microscopically and 16S rRNA sequencing as Streptomyces sp.

Authors and Affiliations

S. R Sukalkar
School of Life Sciences, SRTM University, Nanded, Maharashtra, India
T. A. Kadam
School of Life Sciences, SRTM University, Nanded, Maharashtra, India
H. J. Bhosale
School of Life Sciences, SRTM University, Nanded, Maharashtra, India

Chitin, Chitinase, Streptomyces sp, substrate specificity.

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Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 399-404
Manuscript Number : IJSRST173899
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

S. R Sukalkar, T. A. Kadam, H. J. Bhosale, " Isolation of Chitinase Producing Streptomyces Sp. M1 for Recycling of Fungal Biomass", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 3, Issue 8, pp.399-404, November-December-2017.
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