Phytochemical Screening of leaf extract of Syzygium Cumini L. by HRLC-MS Spectra Method

Authors

  • Satpute S.B.  Department of Biology, Shiv Chhatrapati College, N- 3, Cidco, Aurangabad, Maharashtra, India
  • Vanmare D.J.  Department of Botany, Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Samarthnagar, Aurangabad, Maharashtra, India

DOI:

https://doi.org//10.32628/IJSRST1840118

Keywords:

Syzygium cumini, Phytochemical, antioxident, anti-inflammentary activity, soxhlet and bioactive.

Abstract

By using HRLC-MS spectra method, Phytochemical screening of leaves of Syzygium cumini L. were carried out. It is highly sensitive and used for rapid identification of bioactive compounds. These mass spectra are fingerprint of that compound which can be identified from the data library. It may be the first report of its kind to analyze the bioactive compounds of Syzygium cumini L. The phytochemical study indicates the presence of important bioactive compounds which can be used to treat many diseases in medical field. Phytochemical analysis of methanolic leaf extract of Syzygium cumini L. spectrum profile shows total 80 compounds, out of that 19 major compounds were confirmed on the basis of their retention time, mass, molecular formula and mass per charge ratio (m/z). The findings of this study suggested that, Syzygium cumini L. is a source of important bioactive compounds. It may be contributing natural antimicrobial, antioxident, anti-inflammentary activity and further investigations which may lead to the development of drug formulation.

References

  1. Abhishek Kumar Sah and Vinod K. Verma (2011). Syzygium cumini: An overview. J. Chem. Pharm. Res. 3(3):108-113.
  2. Baris, O., Gulluce, M., Sahin, F., Ozer, H., Kilic, H., Ozkan, H., Sokmen, M., Ozbek, T. (2006). Biological activities of the essential oil and methanol extract of Achillea biebersteinii afan (Asteraceae). Turk. J. Biol., 30: 65-73.
  3. Bhandary, M.J.; Chandrashekar, K.R.; Kaveriappa, K.M. (1995). Medical ethnobotany of the siddis of Uttara Kannada district, Karnataka, India. J. Ethnopharmacol., 47: 149-158.
  4. Chaudhary, B. and Mukhopadhyay, K. (2012). Syzygium cumini (L.) Skeels: A potential source of nutraceuticals. 2(1):46-53.
  5. Gowri, S.S. and Vasantha, K. (2010). Phytochemical screening and antibacterial activity of Syzygium cumini L. (Myrtaceae) leaves extracts. Int. J. Pharm. Tech. Res. 2:1569-157.
  6. Imelda L. Ramos and Teresa May B. Bandiola (2017). Phytochemical Screening of Syzygium Cumini (Myrtaceae) Leaf Extracts Using Different Solvents of Extraction. Der Pharmacia Lettre, 9 (2):74-78.
  7. Kadam V.B., Momin R.K., Tambe S.S. and Wadikar M.S. (2013). Biochemical evaluation of some medicinal plants of marathwada region in Maharashtra. Int. J. pharmaceutical res. and bio sci. 2(4):181-188.
  8. Kakad Subhash, A.S. Wabale and M.N. Kharde (2013). Phytochemical Screening and Antimicrobial Studies on Plumbago zeylanica L. Adv. Biores., 4 (3): 115-117
  9. Muniappan A. and Pandurangan S. (2012). Syzygium cumini L. Skeels: A review of its phytochemical constituents and traditional uses. Asian. Pac. J. Trop. Biomed. Mar 2(3): 240-246.
  10. Osbourne, A.E. (1996). Preformed antimicrobial compounds and plant defense against fungal attack. Plant Cell. 8:1821-1831.
  11. Prateek, A., Meena, R.K. and Yadav, B. (2015). Antimicrobial activity of Syzygium cumini. Ind. J. Applied Res. 5(9):63-66.
  12. Rahman, A.U.; Zaman, K. (1989). Medicinal plants with hypoglycaemic activity. J. Ethnopharmacol., 74: 217-220.
  13. Rukshana, M.S., Doss, A. and Kumari Pushpa Rani, T.P (2017). Phytochemical Screening and GC-MS Analysis of Leaf Extract of Pergularia daemia (Forssk) Chiov. Asian J. Pl. Sci. and Res., 7(1): 9-15.
  14. Tanaka, J.C. A., Da silva, C.C., De OLiveira, A.J.B., Nakamura, C.V. and Dias Filho, B.P. (2006). Antibacterial activity of indole alkaloids from Aspidosperma ramiflorum. Braz. J. Med. Biol. Res., 39(3):387-391.
  15. Teixeira, C.C.; Pinto, L.P.; Kessler, F.H.P.; Knijnik, L.; Pinto, C.P.; Gastaldo, G.J.; Fuchs, F.D. (1997). The effect of Syzygium cumini (L.) Skeels on post-prandial blood glucose levels in non-diabetic rats and rats with streptozotocin-induced diabetes mellitus. J. Etnopharmacol. 56, 209-213.
  16. Teixeira, C.C.; Rava C.A.; Silva, P.M.; Melchior, R.; Argenta, R.; Anselmi, F.; Almeida, C.R.C.,; Fuchs, F.D. (2000). Absence of antihyperglycemic effect of jambolan in expetimental and clinical models. J. Etnopharmacol. 71, 343-347.
  17. Ugbabe, G.E, Ezeunala, M.N, Edmond, I.N, Apev, J. and Salawu, O.A. (2010). Preliminary Phytochemical, Antimicrobial and Acute Toxicity Studies of the Stem, bark and the Leaves of a cultivated Syzygium cumini L. (Family: Myrtaceae) in Nigeria. African J. Biotech. 9(41).6943-6947.
  18. Warrier, P.K.; Nambiar, V.P.K.; Ramankutty, C. (1996). Indian Medicinal Plants. Orient Longman Ltd., Hyderabad. P.225-228.

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Published

2018-11-30

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Section

Research Articles

How to Cite

[1]
Satpute S.B., Vanmare D.J., " Phytochemical Screening of leaf extract of Syzygium Cumini L. by HRLC-MS Spectra Method, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 11, pp.33-37, November-December-2018. Available at doi : https://doi.org/10.32628/IJSRST1840118