Genetic Variation in Mutants of Finger Millet ( Eleusine Coracana (L.) Gaertn. ) Var- Co 13 Evaluated by Rapd Markers

Authors

  • Aviya K  Department of Botany, Annamalai University, Annamalai Nagar, Tamil Nadu, India
  • Mullainathan L  Department of Botany, Annamalai University, Annamalai Nagar, Tamil Nadu, India

Keywords:

Induced Mutation, Finger Millet, EMS, DES, RAPD Analysis.

Abstract

The present study was under taken in order to analyze the chemical mutagenesis on finger millet germplasm. In this regard, Co-13 Variety of Finger millet was subjected to different mutagenic concentration for inducing mutagenesis. The M4 plants exposed to EMS and DES to produce clear difference from the control, thus indicating that mutagenic treatment produce polymorphic regions in the Finger millet. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall mutant, dwarf mutant, early maturity mutant, seed colour mutant and high yielding mutant were analyzed with control. Out of ten primers, five primers (OPC04, OPC08, OPA13, OPC12 and OPD08) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 145 products showing an average of 14.5 bands.

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International Journal of Scientific Research in Science and Technology

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Published

2018-09-30

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Volume 4, Issue 10, September-October-2018

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Research Articles

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[1]
Aviya K, Mullainathan L, " Genetic Variation in Mutants of Finger Millet ( Eleusine Coracana (L.) Gaertn. ) Var- Co 13 Evaluated by Rapd Markers, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 10, pp.48-57, September-October-2018.