Effect of Drought Stress in Paspalum Scrobiculatumon L. Biochemical and Compatible Solute Accumulation

Authors

  • Marimuthu. G  Stress Physiology Lab, Department of Botany, Annamalai University, Annamalai Nagar, Tamil Nadu, India
  • P.V. Murali  Stress Physiology Lab, Department of Botany, Annamalai University, Annamalai Nagar, Tamil Nadu, India

Keywords:

Compatible Solute, Drought, Paspalam Scrobiculatum.

Abstract

Drought stress is a major constraint for crop production to the world. Plants response to drought stress by different adaptation and metabolic alternations. A bag culture experiment was conducted to investigate the effect of Different Day Interval Drought (DID) stress on biochemical and compatible solute accumulation of Paspalum scrobiculatum (L.). The P. scrobiculatum variety CO- 1 and CO - 3 were obtained from Tamilnadu Agricultural University (TNAU), Coimbatore, Tamil Nadu and India. The plants were exposed to 3, 4, 5 and 6DID stress from 20 to 60 Days after sowing (DAS) plants. Irrigation at one day interval was kept as control. The plant sample were collected on 40, 60 and 80 DAS and estimate the biochemical contents like protein, proline, amino acid, glycine betane, total sugar and sucrose were analyzed. Drought stress significantly increased in biochemical contents like proline, amino acid, glycine betaine, total sugar and sucrose content but, the protein content were decreased. These results are indicate that plants are altering their osmoregulation and to balance the water content by lowering the water potential. From the results of this investigation it is clear that the CO - 3 variety were more tolerance then CO - 1 variety plants.

References

  1. Abbas, S.R., S.D. Ahmad, S.M. Sabir and A.H. Shah. (2014). Detection of drought tolerant sugarcane genotypes (Saccharum officinarum) using lipid peroxidation, antioxidant activity, glycine-betaine and proline contents. Journal of Soil Science and Plant Nutrition, 14(1), 233-243.
  2. Allen, C.M. and D.W. Hall. (2003). Paspalum. Flora of North America north of Mexico, 25, 566-599.
  3. Arakawa, K., M. Katayama and T. Takabe, (1990). Levels of betaine and betaine aldehyde dehydrogenase activity in the green leaves, and etiolated leaves and roots of barley. Plant and cell physiology, 31(6), 797-803.
  4. Arivalagan. M and R. Somasundaram. (2016). Induction of drought stress tolerance by propiconazole and salicylic acid in Sorghum bicolor is mediated by enhanced osmoregulation, compatible solutes and biochemical accumulation. Journal of Applied and Advanced Research, 1, 41?52.
  5. Arivalagan. M. and R. Somasundaram. (2016). Propiconazole and salicylic acid alleviate effect of drought stress in Sorghum (Sorghum bicolor (L.) Moench) through biochemical and some physiological characters. Journal of Applied and Advanced Research, 2016, 1(3), 1-7.
  6. Arivalagan. M. and R. Somasundaram. (2017). Exogenous application of triazoles modifies growth and biochemical characteristics of Lycopersicon esculentum Mill. under water limited conditions. Journal of scientific Agriculture, 1, 171?181.
  7. Bates. L.S, R.P. Waldron and I.D. Teare. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39, 205-208.
  8. Bayoumi. T.Y., M.H. Eid and E.M. Metwali. (2008). Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology, 7 (14)
  9. Bernt, E. and H.U. Bergmeyer. (1970). l-Glutamat. Bestimmung mit glutamat-dehydrogenase und NAD. Methoden der enzymatischen Analyse, 2, 1659-1663.
  10. Bohnert. H.J and R.J. Jenson. (1996). Strategies for engineering water-stress tolerance in plants, Trends Biotechnology, 14, 89?97.
  11. BOTANIK, Z. (1937). Journal of the Arnold Arboretum, 18, 54.
  12. Cattivelli. L., F. Rizza, F.W. Badeck, E. Mazzucotelli, A.N. Mastrangelo, E. Francia, C. Mare, A. Tondelli and A.M. Stanca. (2008). Drought tolerance improvement in crop plants. Anintegrated view from breeding to genomics. Field Crops Research, 105, 1-14.
  14. Davies. K.J.A. (1987). Protein damage and degradation by oxygen radicals. I. General aspects. Journal of Biochemistry, 262, 9895?9901.
  15. Grieve. C.M. and S.R. Grattan. (1983). Rapid assay for determination of water soluble quaternary ammonium compounds. Plant and Soil, 70(2), 303-307.
  16. Hare. P.D., W.A. Cress and J. Van Staden. (1998). Dissecting roles of osmolyte accumulation during stress. Plant Cell Environment, 21, 535?553.
  17. Iturriaga, G., R. Su?rez and B. Nova-Franco. (2009). Trehalose metabolism: from osmoprotection to signaling. International journal of molecular sciences, 10(9), 3793-3810.
  18. Jain, S., G. Bhatia, R. Barik, P. Kumar, A. Jain and V.K. Dixit. (2010). Anti-diabetic activity of Paspalum scrobiculatum Linn. In alloxan induced diabetic rats. Journal of Ethno pharmacology, 127, 325-338.
  19. Jungklang. J. and K. Saengnil. (2012). Effect of paclobutrazol on patumma cv. Chiang Mai Pink under water stress. Songklanakarin Journal of Science and Technology, 34, 361-366.
  20. Kavikishore. P.B., S. Sangam, R.N. Amrutha, P. Srilaxmi, K.R. Naidu, K.R.S.S. Rao, S.Rao, K.J. Reddy, P. Theriappan and N. Sreenivasulu.? (2005). Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance, Current Science, 88, 424?438.
  21. Kavikishore. P.B., Z. Hong, G.U. Miao, C.A.H. Hu and D.P.S. Verma. (1995). Over expression of 1-pyroline-5-carboxylase synthetase increases proline production and confers osmotolerance in transgenic plants, Plant Physiology, 108, 1387?1394.
  22. Kiran, P., M. Denni and M. Daniel. (2014). Anti-diabetic Principles, Phospholipids and Fixed Oil of Kodo Millet (Paspalum scrobiculatum Linn.). Indian Journal of Applied Research, 4, 13-15.
  23. Lowry. O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193(1), 265-275.
  24. Lum. M.S., M.M. Hanafi, Y.M. Rafii and A.S.N. Akmar. (2014). Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. Journal of Animal and Plant Science, 5, 1487-1493.
  25. Mafakheri. A., A. Siosemardeh, B. Bahramnejad, P.C. Struik and Y. Sohrabi. (2011). Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum) cultivars. AJCS, 5, 1255-1260.
  26. Misra. N. and A.K. Gupta.? (2006). Effect of salinity and different nitrogen sources on the activity of antioxidant enzymes and indole alkaloid content in Catharanthus roseus seedlings. Journal of plant physiology, 163(1), 11-18.
  27. Moore, S. and W.H. Stein. (1948). Photometric nin-hydrin method for use in the ehromatography of amino acids. Journal of biological chemistry, 176, 367-388.
  28. Mostajean, A and V.R. Eichi. (2009). Effects of drought stress on growth and yield of rice (Oryza sativa L.) Cultivars and accumulation of proline and soluble sugars in sheath and blades of their different ages leaves American-Evras. Journal of Agriulture and Environmental Science, 5, 264-272.
  29. Murthy, A.V.S and N.S.A. Subramanyam. (1989). Textbook of Economic Botany. (Wiley Eastern Limited), New Delhi.
  30. Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucose. Journal of biological chemistry, 153(2), 375-380.
  31. O'Kennedy. M.M., A. Grootboom and R.R. Shewry. (2006). Harnessing sorghum and millet biotechnology for food and health. Journal of Cereal Science, 44, 224?235.
  32. Osman. M.E.H., S.S. Elfeky, K. Abo El-Soud and A.M. Hasan. (2007). Response of Catharanthus roseus Shoots to Salinity and Drought in Relation to Vincristine Alkaloid Content. Asian Journal of Plant Sciences, 6(8), 1223-1228.
  33. Perez-Lopez, U., A. Robredo, M.? Lacuesta, A. Munoz-Rueda and A. Mena-Petite. (2010). Atmospheric CO 2 concentration influences the contributions of osmolyte accumulation and cell wall elasticity to salt tolerance in barley cultivars. Journal of plant physiology, 167(1), 15-22.
  34. Phillips. R.L., D.R. Zak, W.E. Holmes and D.C. White. (2002). Microbial community composition and function beneath temperate trees exposed to elevated atmospheric carbon dioxide and ozone. Oecologia, 131(2), 236-244.
  35. Pimentel. C. (2004) The relation of the plant with water. EDUR, Seropedica
  36. Sankar, B., C.A. Jaleel, P. Manivannan, A. Kishorekumar, R. Somasundaram and R. Panneerselvam. (2007). Effect of Paclobutrazol on water stress amelioration through antioxidants and free radical scavenging enzymes in Arachis hypogaea L. Colloids and Surfface. B: Biointerfaces, 60, 229?235
  37. Shao. H.B., L.Y. Chuc, G. Wu, J.H. Zhang, Z.H. Lua and Y.C. Hug. (2007). Changes of some anti-oxidative physiological indices under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at tillering stage. Colloids Surfface B: Biointerfaces, 54, 143?149.
  38. Tan, Y., Z. Liang, H. Shao and F. Du. (2006). Effect of water deficits on the activity of anti-oxidative enzymes and osmoregulation among three different genotypes of Radix Astragali at seeding stage. Colloids and surfaces B: Biointerfaces, 49(1), 60-65.
  39. Tuna. A.L., C. Kaya and M. Ashraf. (2010). Potassium sulfate improves water deficit tolerance in? melon plants grown under glasshouse conditions. Journal of Plant Nutrition, 33, 1276-1286.
  40. Yadav. S.K., N. Jyothi Lakshmi, M. Maheswari, M. Vanaja and B. Venkateswarlu. (2005). Influence of water deficit at vegetative, an thesis and grain filling stages on water relation and grain yield in sorghum. Indian Journal of Plant Physiology, 10, 20-24.
  41. Yadava, H. S., & Jain, A. K. (2006). Advances in kodo millet research. Directorate of Information and Publications of Agriculture, Indian Council of Agricultural Research.
  42. Zhang. L.H., J.H. Lai, Z.S. Liang and M. Ashraf. (2014). Interactive Effects of Sudden and Gradual Drought Stress and Foliar-applied Glycine betaine on Growth, Water Relations, Osmolyte Accumulation and Antioxidant Defence Systemin Two Maize Cultivars Differing in Drought Tolerance. Journal of Agriculture and Crop Science, 200, 425?433.
  43. Zlatev. Z and F.C. Lidon. (2012). An overview on drought induced changes in plant growth, water relations and photosynthesis. Emirate Journal of Food and Agriculture, 24, 57-72.

International Journal of Scientific Research in Science and Technology

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Published

2018-02-28

Issue

Volume 4, Issue 2, January-February-2018

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Research Articles

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How to Cite

[1]
Marimuthu. G, P.V. Murali, " Effect of Drought Stress in Paspalum Scrobiculatumon L. Biochemical and Compatible Solute Accumulation, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.618-622, January-February-2018.