Influence of the Topical Application Acetone Solution of Vitamin A (Retinol) to the Fifth Instar Larvae of the Silkworm, Bombyx   mori (L) (Race : PM x CSR2) on the Economic Parameters

Authors

  • Manali R. Shinde  Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar; Tal. Baramati, Dist. Pune, Maharastra, India
  • Shrutika L. Salunkhe  Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar; Tal. Baramati, Dist. Pune, Maharastra, India
  • Shubhangi S. Pawar  Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar; Tal. Baramati, Dist. Pune, Maharastra, India
  • Vitthalrao B. Khyade  Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar; Tal. Baramati, Dist. Pune, Maharastra, India

Keywords:

Retinol, TSI, Shell Ratio, Denier Scale of Silk Filament.

Abstract

The attempt concerned with efficient topical application of acetone solution of Vitamin A (retinol) to the fifth instar larvae of multivoltine silkworm, Bombyx mori (L) (Race: PM x CSR2) and assessment of economic parameters. The experimentatio was carried at laboratory level. The ten microliters of one milligram per liter strength acetone solution of retinol was topically applied to the individual fifth instar larvae of multivoltine cross breed race of silkworm, Bombyx mori ( L ) ( PM x CSR2) variously at 48, 54, 60 and 66 hours after the fourth moult. Topical application of acetone solution of retinol was found variously reflected into prolongation of larval age (14 - 39 %); improvement in the tissue somatic index (TSI) of silk glands (3.06 - 3.343); cocoon shell ratio (2.790 - 3.177) and denier scale of silk filament (p< 0.01). Topical application of acetone solution of retinol, thus chiefly lengthen the larval age in silkworm, Bombyx mori (L). Time required for eating and amount of mulberry leaves eaten had both been increased and they were practically reflected into the improvement in the quality of cocoon and the silk filament. Diterpenoid nature of retinol and delay in the spinning among the larvae, recipient of retinol in the attempt indicate the possibility of insect juvenoid activity of retinol in silkworm, Bombyx mori (L). Efficient utilization of retinol, the diterpenoid through acetone solvent for topical application to the fifth instared larvae of silkworm, Bombyx mori (L) may open a new biotechnological avenue in the sericulture industries

References

  1. Angelica Nakamura; Renata Stiebler; Marcelo R Fantappié; Eliane Fialho; Hatisaburo Masuda and Marcus F Oliveira (2007). Effects of retinoids and juvenoids on moult and on phenoloxidase activity in the blood-sucking insect, Rhodnius prolixus (L). Acta Trop. 2007 Jul 1;: 17686447 Cit:1 http://lib.bioinfo.pl/paper:17686447.
  2. Riddiford, L. M. and Ashburner, M. (1990). Role of Juvenile hormone in larval development and metamorphosis in Drosophila melanogaster (L). Comparative Endocrinology. 82: 172-183.
  3. Bowles, J. ; Knight, D. ; Wihlem, D. ; Richman, J. ; Mamiya, S. ; Yashiro, K. ; Chawengsaksphak, K. ; Wilson, M. J. ; Rossant, J. ; Hamada, H. and Koopman, P. (2006). Retinoid signaling determines germ cell fate in mice. Science 312: 596 – 600.
  4. Ying, H. Wang ; Guirong Wang and Gerold A. LeBlanc (2006). Cloning and characterization of the retinoid X receptor from a primitive crustacean, Daphnia magna (L). General and Comparative Endocrinology. 150: 309 – 318. www.sciencedirect.com and www.elsevier.com/Locate/ygcen
  5. Akai, H. and Kobayashi, M. (1971). Introduction of prolonged larval instar by juvenile hormone in Bombyxmori(L) ( Lepidoptera : bombycidae ). Appl. Entemol. Zool, 6: 1938 – 1939.
  6. Calvez, B.; Hiren, M. and Reddy, M. (1976). Progress of developmental programme during the last larval instar Bombyxmori (L): Relationships with food intake, ecdysteroids and juvenile hormone. Journal of insect physiology,24 (4): 233-239.
  7. Ching, F.C; Mukakeshi, S. and Tamura,S. (1972). Giant cocoon formation in the silkworm, Bombyxmori (L) treated with methyloneoxyphenyl derivatives. Agar. Biol. Chem. 36: 692-694.
  8. Granier and Granier (1938). Fenoxycarb, a fairly new growth regulator: A review of its effects on insects. Ann. Appl. Biol. 122: 369-403.
  9. Kamada, A. and Shimada, S. (1988). Effects of methoprene on growth profile of larval organs of silkworm, Bombyxmori (L). Jornal of sericulture science, Jpn. 48 (2): 129-136.
  10. Khyade V.B. (2004). Influence of juvenoids on silkworm, Bombyxmori (L). Ph.D thesis, Shivaji University, Kolhapur. ( Maharashtra, India ).
  11. Kiguchi, K. and Augi, N. (1981). Ecdysteroid level and development events during larval moulting in silkworm, Bombyxmori (L). insect physiol. 26: 805-813.
  12. Krishnaswami, S.; Narimhan, N.; Suryanarayana, S.K and Kumararaja, S. (1978). Manual on sericulture, vol. 2: silkworm rearing, FAO publication, agricultural services bulletin no. 15.
  13. Mamatha, D.N; Nagalalakhamma, K.; Vijay, P. and RajeshwaraRao, A.M. (1999). Impact of selected juvenile hormone mimics on organic constituents of silkworm, Bombyxmori (L). proceedings of NSTS- 99: 185-186.
  14. Muroga , A.; Nakajima, M.; Aomori,S.; Ozawa, Y. and Nihmura, M. (1975). Utilization of synthetic juvenile hormone analogue for the silkworm rearing on the mulberry leaves. J. Sreic. Sci. Jpn. 44: 267-273.
  15. Nihmura, M.; Aomori,S.; Mori, K. and Matusui, M. (1972). Utilization of synthetic compounds with juvenile hormone activity for silkworm rearing. Agri. Biol. Chem. 36: 882-889.
  16. Normn, T.J and Bailey, (1995). Stastical methods in biology.
  17. Prabhu, V.K.K; John, M. (1975). Juvenomimetic activity in some plants. Experinential,31: 913-914.
  18. Rajashekhargouda, R. (1991). Studies on methods to increase silk yield of Bombyxmori (L) ( Lepidoptera : bombycidae ). Ph.D thesis, Tamil Nadu University, Coimbtore, India.
  19. RatnaSen, (1988). How does juvenile hormone analogue cause more silk yield. Indian silk: 21-22.
  20. Riddiford , L.M (1985). Hormone action at Cellular and Molecular actions of juvenile hormone: general considerations for premetamorpic actions. Adv. Insect physiol., 24: 213-214.
  21. Sehnal, F. and Rambold, H. (1985). Brain stimulation of juvenile hormone production in insect larvae. Experimentation 41: 684-685.
  22. Slama, K. (1971). Insect juvenile hormone analogues ann. Rev. biochem. 40: 1079-1102.
  23. Slama, K.; Wimmer, Z. and Romanuk, M. (1978). Juvenile hormone activity of some glycosidicjuvenogens. Hopperstyler’s Physiol. Chem. 359: 1407-1412.
  24. Slama, K. (1985). Pharmacology of insect juvenile hormones. In: Biochemistry and pharmacology ( Eds G.A Kerkutand L.I Gilbert ), vol. 11: 357-394. Pergamon Press, New York.
  25. Vitthalrao B. Khyade; Patil, S.B; Khyade,, S.V and Bhavane, G.P (2002). Influence of acetone maceratives of Vitisvinifera on the larval parameters of silkworm Bombyxmori (L). Indian journal of comparative animal physiology vol. 20: 14-18.
  26. Vitthalrao B. Khyade; Patil, S.B; Khyade, S.V and Bhavane, G.P (2003). Influence of acetone macerative of Vitisvinifera on the economic parameters of silkworm Bombyx mori (L). Indian journal of comparative animal physiology. Vol. 21: 28-32.
  27. Wimmer, Z. and Romanauk, M. (1981). The synthesis of biologically active 2-(4- hydroxyl benzyl)-1- cyclohexanol derivation. Coll. Czech. Chem. Commun. 46: 2573-2586.
  28. Anjang, Tan ; Hiromasa, Tanaka; Toshiki Tamura and Takahiro Shiotsuki (2005). Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase. Proc. Natl. Acad. Sci. USA Aug 16, 2005; 102(33): 11751 – 11756. http://genepath.med.harvard.edu/~perrimon/papers/2013_Zirin_DevBio_Ecdysone.pdf

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Published

2018-02-28

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Research Articles

How to Cite

[1]
Manali R. Shinde, Shrutika L. Salunkhe, Shubhangi S. Pawar, Vitthalrao B. Khyade, " Influence of the Topical Application Acetone Solution of Vitamin A (Retinol) to the Fifth Instar Larvae of the Silkworm, Bombyx   mori (L) (Race : PM x CSR2) on the Economic Parameters, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.792-799, January-February-2018.