Response of the Microbial Activity to Fosetyl-Aluminium and Copper Oxychloride (Fungicides) in Groundnut (Arachis Hypogaea L.) Soils

Authors

  • A. Rekha Padmini  Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India
  • B. Anuradha  Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India
  • A. Madhavi  Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India
  • Rangaswamy V  Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India

Keywords:

Microbial activity , Protease enzyme, fungicides (fosetyl-aluminium and copper oxychloride and groundnut (Arachis hypogaea L.) soils

Abstract

The evaluation of the adverse effect of Fungicides on the microbial community and the soil enzyme activity were evidenced in soil amended with fosetyl-aluminium and copper oxychloride (Fungicides) in groundnut (Arachis hypogaea L.) soils. Variations in activity were independent upon the period of incubation. A fungicide disturbs the activities of soil enzymes and soil micro biota. So we investigated in laboratory conditions that the effect of two fungicides, fosetyl-aluminium and copper oxychloride on enzyme activities, such as protease in two soils collected from groundnut (Arachis hypogaea. L) cultivated fields of Anantapuram district of Andhra Pradesh, India, by conducting experiments at different concentrations (10, 25, 50, 75 and 100 ppm) which are equivalent to field application rates (1.0, 2.5, 5.0, 7.5 and 10.0 kg ha-1). In our present study we observed, protease activities were significantly enhanced at 2.5 and 5.0 kg ha-1 in black and red soils after 10 days of incubation. Furthermore increase in concentration of fungicides and decreased the rate of enzyme activities. However, the stimulatory effect was continued up to 20 days of incubation in black and red soils. Whereas, the decline phase was started after 20 days and the minimum enzyme activities were noticed at the end of 40 days of incubation. But higher concentrations of fungicides at the level of 7.5 to 10.0 kg ha-1 were either toxic or innocuous to protease activity in black and red soils. An increasing trend in the activity of microbial population and soil enzyme activities were observed during 20 days of incubation in both control and treated soil with fungicides. These fluctuations in activities were in accordance to impact of selected fungicides in groundnut (Arachis hypogaea L.) soils.

References

  1. Anonymous Report, 2016 - 17, Department Of Agriculture, Operation and farmers welfare. Ministry of Agriculture and Farmers Welfare Government of India Krishi Bhawan, New Delhi-110 001.
  2. Bundt M, Widmer F, Pesaro M, Zeyer J and Blaser P. 2001. Preferential flow paths: biological ‘hot spots’ in soils. Soil Biology & Biochemistry. 33: 729-738.
  3. Fierer N, Bradford M A and Jackson R B 2007. Towards an ecological classification of soil bacteria. Ecology.88: 1354-1364.
  4. Burns, R.G. 1978. Soil Enzymes. Academic Press, London.
  5. Burns, R.G. and Dick, R.P. 2002. Enzymes in the Environment: Activity, Ecology and Applications. Marcel Dekker, New York.
  6. Amador, J.A., Glucksman, A.M., Lyons, J.B. and Gorres, J.H. 1997. Spatial distribution of soil phosphatase
  7. activity within a riparian forest. Sci. 162: 808-825.
  8. Waldrop, M.P., Balser, T.C. and Firestone, M.K. 2000. Linking microbial community composition to function
  9. in a tropical soil. Soil Biol. Biochem. 32: 1837-1846.
  10. Kourtev, P.S., Ehrenfeld, J.G. and Haggblom, M. 2002. Exotic plant species alter the microbial community structure and function in the soil. Ecology 83: 3152-3166.
  11. Sinsabaugh, R.L., Carreiro, M.M. and Repert, D.A. 2002. Allocation of extracellular enzymatic activity in relation to litter composition, Ndeposition, and mass loss. Biogeochemistry 60: 1-24.
  12. Bolton Jr., H., Smith, J.L. and Link, S.O. 1993. Soil microbial biomass and activity of a disturbed and undisturbed shrub-steppe ecosystem. Soil Biol. Biochem. 25: 545-552.
  13. Eivazi, F. and Bayan, M.R. 1996. Effects of long term prescribed burning on the activity of selected soil enzymes in an oak-hickory forest. Can. J. For. Res. 26: 1799-1804.
  14. Garcia, C. and Hernandez, T. 1997. Biological and biochemical indicators in derelict soils subject to erosion. Soil Biol. Biochem. 29: 171-177.
  15. Boerner, R.E.J., Decker, K.L.M. and Sutherland, E.K. 2000. Prescribed burning effects on soil enzyme activity in a southern Ohio hardwood forest: a landscape-scale analysis. Soil Biol. Biochem. 32: 899-908.
  16. Tscherko, D., Rustemeier, J., Richter, A., Wanek, W. and Kandeler, E. 2003. Functional diversity of the soil microflora in the primary successon across two glacier forelands in the Central Alps. Eur. J. Soil Sci.54:685-696.
  17. Pan-UK. (2003). Current pesticide spectrum, global use and major concerns.
  18. Pimentel, D. 1995. Amounts of pesticides reaching target pests: Environmental impacts and ethics. J. Agric. Environ. Ethics. 8:17-29.
  19. Carriger, J. F., Rand, G. M., Gardinali, P. R., Perry, W. B., Tompkins, M. S. and Fernandez, A. M. 2006.
  20. Pesticides of potential ecological concern in sediment from South Florida Canals: An ecological risk prioritization for aquatic arthropods. Soil Sed. Contam. 15: 21-45.
  21. Andrea, M. M., Peres, T. B., Luchini, L. C. and Pettinelli, A. Jr. 2000). Impact of long term pesticide application on some soil biological parameters. J. Environ. Sci. Health B 35: 297-307.
  22. Baxter, J. and Cummings, S. P. 2008. The degradation of the herbicide bromoxynil and its impact on bacterial diversity in a top soil. J.Appl. Microbiol. 104: 1605-1616.
  23. Ingram, C. W., Coyne, M. S. and Williams, D. W. 2005. Effects of commercial diazinon and imidacloprid on microbial urease activity in soil. J. Environ. Qual. 34: 1573-1580.
  24. Littlefield-Wyer, J. G., Brooks, P. and Katouli, M. 2008. Application of biochemical fingerprinting and fatty
  25. acid methyl ester profiling to assess the effect of the pesticide. Atradex on aquatic microbial
  26. communities.Environ. Pollut. 153: 393-400.
  27. Niewiadomska, A. 2004. Effect of carbendazim, imazetapir and thiramon nitrogenase activity, the number of microorganisms in soil and
  28. yield of red clover (Trifolium pretense L). Pol. J.Environ. Stud. 13: 403-410.
  29. Wang, M. C., Gong, M., Zang, H. B., Hua, X. M., Yao, J., Pang, Y. J. and Yang, Y. H. 2006. Effect of methamidophos and urea application on microbial communities in soils as determined by microbial biomass and community level physiological profiles. J. Environ. Sci. Health B. 41: 399-413.
  30. Demanou, J., Sharma, S., Weber, A., Berndt-Michae, W., Njine, T., Monkiedje, A., Munch, J. C. and Schloter, M. 2006. Shifts in microbial community functions and nitrifying communities as a result of combined application of copper and mefenoxam. FEMS Microbiol. Lett. 260:55-62.
  31. Kinney, C. A., Mandernack, K. W. and Mosier, A. R. 2005. Laboratory investigations into the effects of the pesticides mancozeb, chlorothalonil and prosulfuron on nitrous oxide and nitric oxide production in fertilized soil. Soil Biol. Biochem. 37: 837-850.
  32. Antonious, G. F. 2003. Impact of soil management and two botanical insecticides on urease and invertase activity. J. Environ. Sci. Health B.38: 479-488.
  33. Hussain, S., Arshad, M., Saleem, M. and Khalid, A. 2007 a. Biodegradation of a- and b-endosulfan by soil bacteria. Biodegradation 18: 731-740.
  34. Hussain, S., Arshad, M., Saleem, M. and Zahir, Z. A. 2007 b. Screening of soil fungi for in vitro degradation of endosulfan. World J. Microbiol. Biotechnol. 23: 939-945.
  35. Kumar, M. and Philip, L. 2006. Enrichment and isolation of a mixed bacterial culture for complete mineralization of endosulfan. J. Environ.Sci. Health B. 41: 81-96.
  36. Siddique, T., Okeke, B. C., Arshad, M. and Frankenberger, W. T. Jr. 2003. Enrichment and isolation of endosulfan-degrading microorganisms. J. Environ. Qual. 32: 47-54.
  37. Tyess, D. L., Shea, P. J. and Parkhurst, A. M. 2006. Mineralization potential of atrazine and degradation intermediates from clustered characteristics in inoculated soils. Soil Sed. Contam. 15: 87-102.
  38. Das, A. C. and Mukherjee, D. 2000 a. Soil application of insecticides influences microorganisms and plant nutrients. Appl. Soil Ecol. 14:55-62.
  39. Das, A. C. and Mukherjee, D. 2000 b. Influence of insecticides on microbial transformation of nitrogen and phosphorus in Typic Orchra gualf soil. J. Agric. Food Chem. 48: 3728-3732.
  40. Widenfalk, A., Bertilsson, S., Sundh, I. and Goedkoop, W. 2008. Effects of pesticides on community composition and activity of sediment microbes—Responses at various levels of microbial community organization. Environ. Pollut. 152: 576-584.
  41. Johnson CM and Ulrich A 1960. Determination of moisture in plant tissues. In: Wilde SA et al (eds). Soil and
  42. Plant analysis for tree culture. Obortage publishing Co., Oxford, pp: 112-115.
  43. Alexander, M. 1961.Introduction to soil microbiology, 2nd edn.Wiley, Estern Ltd, New Delhi. ackson, M.L. 1971. Soil chem. anal. Prentice Hall India, New Delhi.
  44. Barnes H and Folkard B R. 1951.The determination of nitrite. Analyst 76:599-603.
  45. Ranney T A and Bartlett R J. 1972. Rapid field determination of nitrate in natural waters. Commun Soil Sci Plant Anal. 3:183-186.
  46. Speir, T.W and D.J. Ross. 1975. Effects of storage on the activities of protease, urease, phosphatase and sulphatase in three soils under pasture. N.Z.J. Sci. 18: 231- 237.
  47. Jayamadhuri , R. 2002. Interactions between fungicides and microorganiams in groundnut (Arachis hypogeae
  48. L.) soils. M.Phil., Dissertation, submitted to Sri Krishnadevaraya University, Anantapur.
  49. Jayamadhuri , R. 2004. Interactions between pesticides and microorganiams in soils from groundnut fields.
  50. Ph.D., Thesis, submitted to Sri Krishnadevaraya University, Anantapur.
  51. M. Megharaj, I. Singleton, R. Kookana, and R. Naidu. 1999. “Persistence and effects of fenamiphos on native algal populations and enzymatic activities in soil,” Soil Biology and Biochemistry, vol. 31. no. 11: pp. 1549-1553.
  52. Anonymous. 2011. Agriculture production plan for Anantapur District, Andhra Pradesh, India. Department of Agriculture, Anantapur, Andhra Pradesh, India.
  53. Loganathan M, Sundarababu P C and Balasubramanyam G. 2002. Efficacy of biopesitcides against
  54. Spdeoptera litura (Fab.) on groundnut (Arachis hypogaea L.). Mad Agric J 89(7-9):521-524.
  55. Srinivasulu M, Mohiddin G J and Rangaswamy V. 2012. Effect of insecticides alone and in combination with fungicides on nitrification and phosphatase activity in two groundnut (Arachis hypogaea L.) soils. 
  56. Environ Geochem Health 3(34):365-374.
  57. Kiss S, Dragon-Bularda M and Radulescu D. 1975. Biological signifcance of enzymes in soil. Adv Agron 27: 25-87.
  58. Dedeken M and Voets J P. 1965. Recherches sur le metabolisme des acides amines dans le sol. 1. Le metabolisme de glycine, alanine, acids aspartque et acide glutaminique, Annales de 1’ Institut Pasteur, Paris
  59. 10g 3: 103-111.
  60. Ladd J N and Butler H A. 1972. Short-term assays of soil proteolytic enzyme activities using proteins and dipeptide derivatives as substrates. Soil Biol Biochem. 4: 19-30.
  61. Watanabe K and Hayano K. 1993. Distribution and identifcation of proteolytic Bacillus sp. in paddy field under rice cultivation. Can. J. Microbiol. 39: 674-680.
  62. Rangaswamy .V, Reddy B. R and Venkateswarlu . K. 1994. Activities of dehydrogenase and protease in soil as in?uenced by monocrotophos, quinalphos, cypermethrin, fenvalerate. Agric Ecosys Environ. 47: 319-326.
  63. Nasreen C, Mohiddin G J, Srinivasulu M, Rekha Padmini A and Ramanamma P. 2012. Interaction effects of insecticides on enzyme activities in black clay soil from groundnut (Arachis hypogaea L.) felds. Environ Res Eng Management. 2:21-28.
  64. Ramudu AC, Srinivasulu M, Mohiddin G J and Rangaswamy V. 2012. Effect of fungicides on urease and protease activities in two groundnut (Arachis hypogaea L.) soils. Int. J. Environ Protection. 2: 23-28.

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Published

2018-02-28

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Research Articles

How to Cite

[1]
A. Rekha Padmini, B. Anuradha, A. Madhavi, Rangaswamy V, " Response of the Microbial Activity to Fosetyl-Aluminium and Copper Oxychloride (Fungicides) in Groundnut (Arachis Hypogaea L.) Soils, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.773-783, January-February-2018.