Detection of Cholera Toxin Using Lactose-Decorated Silver Nanoparticles

Authors

  • Ratnadeep R. Koyale  Department of Biotechnology, Sinhgad College of Science, Ambegaon Bk, Pune, Maharashtra, Pune, Maharastra, India
  • Imran L. Patel  Department of Biotechnology, Sinhgad College of Science, Ambegaon Bk, Pune, Maharashtra, Pune, Maharastra, India
  • Samradni D. Pingale  Department of Biotechnology, Sinhgad College of Science, Ambegaon Bk, Pune, Maharashtra, Pune, Maharastra, India

Keywords:

Biosensor, Cholera toxin, Surface Plasmon Absorbance, Zeta Potential.

Abstract

A novel method for biosensor preparation is mechanized, which uses silver nanoparticles as sensing molecules. These nanoparticles were prepared using banana leaf extract-mediated synthesis, which is decorated using PEG to stabilize their surface. The silver nanospheres were synthesized with size 100 to 120 nm enveloped with PEG coating with the stability of -16 mV. They were characterized using UV-spectroscopy, particle size analyzer and SEM imaging. These particles are coated with lactose decorated silver nanoparticles against food pathogen such as cholera toxin. The food samples were exposed to these plates and the surface plasmon absorbance was recorded at 419 nm. Changes in surface Plasmon absorbance showed biosignals that specify the presence of toxin producing bacteria in the sample.

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International Journal of Scientific Research in Science and Technology

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Published

2018-02-28

Issue

Volume 4, Issue 2, January-February-2018

Section

Research Articles

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How to Cite

[1]
Ratnadeep R. Koyale, Imran L. Patel, Samradni D. Pingale, " Detection of Cholera Toxin Using Lactose-Decorated Silver Nanoparticles, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.902-906, January-February-2018.