The Genotoxic Effect of Coal Fly Ash of Thermal Power Plant on Raphanus sativus L. ( Radish )

Authors

  • Parimal M. Patel  Department of Biosciences, Veer Narmad South Gujarat University, Surat, Gujarat, India.
  • Rajendra Jadav  Bhavan's Sheth R. A. College of Science, Khanpur, Ahemdabad, Gujarat, India
  • Kailash Patel  Department of Biosciences, Veer Narmad South Gujarat University, Surat, Gujarat, India.

Keywords:

Coal Fly-Ash, Genotoxic, Cytogenetic, Mitotic Index, Raphanus Sativus L.

Abstract

The effect of coal fly ash treatment on the chromosomes of Raphanus sativus L. was investigated. The seeds of Raphanus sativusL. were placed in petri dishes in three replicates and allowed to germinate for five days in different concentration of coal fly ash solution. The root were treated with diluted, semi-diluted, and concentrated solution of fly ash, while the control group had distilled water. The total aberration were examined. The mitotic index was calculated. The mitotic index decreased as the concentration increased. The highest mitotic index value was diluted fly ash solution while the least was concentrated fly ash treatment. The results show the most frequent chromosomal abnormalities observed included: chromatid bridge, c-mitosis, and stickiness. Concentrated fly ash solution is much more genotoxic than semi-diluted fly ash solution, as it induced more aberrations having percentage abnormalities for the highest concentration tested.

References

  1. Davison, R.L., Natusch D.F.S., Wallace, J.K. and Evans, C.A. Jr. 1974. Trace elements in fly-ash : dependence of concentration on particle size. Environ Sci. Technol. 8 : 1107-1113.
  2. Page, A.L., elseewi, A.A. and Straughan, I. 1979. Physical and chemical properties of fly-ash from coal fired power plants with reference to environmental impacts. Residue Rev. 71 : 83-120.
  3. Khan, S., Bagum, T. and Singh, J. 1996. Effect of fly-ash on physico-chemical properties and nutrient status of soil. Indian J. Environ Health. 38 : 41-46.
  4. Gupta, S.K., Tewari, A., Srivastava, R., Murthy, R.C. and Chandra, S. 2005. Potential of Eisenia foetida L. for sustainable and efficient vermi composting of fly-ash, water, Air and Soil pollution. 163 : 293-302.
  5. Pandey, V.C. and Singh, N. 2010. Impact of fly-ash incorporation in soil systems. Agriculture, Ecosystems and Environment. 139 : 16-27.
  6. Giedrojc, B., Fatyga, J. and Hryncewizc, Z. 1980. The effect of fertilizers with ashes from black coal burned in electric power stations of the properties of sandy soils and crops. Polish. J. Soil. Science. 13 : 163-171.
  7. Pandey, V.C., Abhilash, P.C., Upadhyay, R.N. and Tewari, D.D. 2009. Application of fly-ash on the growth performance and translocation of toxic heavy metals within Cajanus cajan L. Implication for safe utilization of fly-ash for agricultural production, Journal of hazardous materials. 166 : 255-259.
  8. Hodgson, L., Dyer, D. and Brown, D.A. 1982. Neutralization and dissolution of high calcium fly-ash. J. Environ. Qual. 11 : 93-98.
  9. Campbell, D.J., Fox, W.E., Aitken, R.L. and Bell, L.C. 1983. Physical characteristics of san amended with fly-ash. Australian. J. soil research. 21 : 147-154.
  10. Miller, D.M., Miller, W.P., Dudka, S. and Somner, M.E. 2000. Characterization of industrial by products : Land application of agricultural industrial and municipal by product. SSSA book series. Madison. WI. PP : 107-119.
  11. Davies, B.E. 1993. Radish as an indicator plant for derelict land : uptake of zinc at toxic concentrations. Comm. Soil. Sci. Plant. Anal. 24 : 1883-1895.
  12. Sahi, A.N. and Singh, R.N. 1996. Fly-ash induced chromosomal aberrations in Allium cepa, cytobios. 86 : 3-4 , 23-28.
  13. Mishra, A. and Choudhari, M.A. 1999. Effect of salicylic acid on heavy metal induced membrane deterioration mediated by lipooxygenase in rice. Biol. Plant. 42 : 409-415.
  14. Taets, C. and Rayburn, A.L. 1996. Coal fly-ash exposure at agronomic levels does not induced triploidy in maize. Bull. Environ. Contam. Toxicol. 56 : (5), 690-695.
  15. Mc Murphy, L.M., Birdar, D.P., Tacts, C. and Rayburn, A.L. 1996. Differential effect of weathered coal fly-ash and fly-ash leachete on the maize genome. Arch. Environ. Contam. Toxicol. 31 : (2), 166-169.
  16. Kumar, G. and Srivastava, S. 2006. Mechanism of Aluminum cytotoxicity in meristematic cells of Helianthus annuus L. J. Phytol. Res. 19 : 175-178.
  17. Kumar, G. and Tripathi, R. 2007. Lead induced cytotoxicity and mutagenecity in Grass Pea Turk. J. Biol. Sci. 32 : 73-78.
  18. Jiang, W. and Liu,k D. 2000. Effect of Pb on root growth, Cell division and nucleolus of Zea mays L. Bull. Environ. Contam. Toxicol. 65 : 786-793.
  19. Caetano-Pereira, C.M., Pagliarini, M.S. and Brasil, E.M. 1995. Influence of aluminium in causing chromosome stickiness in maize microsporocytes. Maydica. 40 : 325-330.
  20. Kumar, G. and Tripathi, A. 2003. Comparative mitotoxicity and mutagenicity of two heavy metals in Allium cepa L. J. Cytol. Genet-4(NS) : 169-172.
  21. Kumar, G. and Srivastava, S. 2006. Mechanism of Aluminum cytotoxicity in meristematic cells of Helianthus annuus L. J. Phytol. Res. 19 : 175-178.
  22. Patnaik, S., Saran, B.L. and Patnaik, S.M. 1984. Effect of Zarda ( processed tobacco leaf ) extract on the chromosomes of Allium cepa Cyotlogia. 49 : 807-814.

International Journal of Scientific Research in Science and Technology

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Published

2016-11-30

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Volume 2, Issue 6 , November-December-2016

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Research Articles

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How to Cite

[1]
Parimal M. Patel, Rajendra Jadav, Kailash Patel, " The Genotoxic Effect of Coal Fly Ash of Thermal Power Plant on Raphanus sativus L. ( Radish ), International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 2, Issue 6 , pp.555-561, November-December-2016.