Invitro Screening and Molecular Characterization of Trichoderma Harzianum Against Phytophthora Palmivora (Butl.,) Causing Root Rot Disease in Caricapapaya L.

Authors(4) :-M. Mahadevi, A. Thavaselvi, V. Latha, A. Panneerselvam

The present investigation showed Trichoderma. harzianum exhibited promising biocontrol ability against P. palmivora (Butl.) by dual culture experiment and poisoned food technique. T. harzianum was found to be the most effective antagonists against P. palmivora (Butl.). GC-MS analysis of ethyl acetate extract of the filtrate of T. harzianum revealed the presence of 17 compounds by representing 17 prominent peaks. The major phytocompound of T. harzianumwas Diethyl Phthalate (RT= 4.105 min) with 29.994 % of peak area.The ITS region gene sequence of potential biocontrol fungus obtained in this study were deposited in GenBank under the accession number KY346985. The sequence of potential biocontrol fungus showed the maximum homology with T. harzianum (GenBank Accession Number KC33021) by BLAST homology analysis. Therefore potential biocontrol fungus was further confirmed as T. harzianum by molecular analysis. The secondary structure of ITS region gene of T. harzianum showed 45 stems, 28 bulge loops and 12 hairpin like structure respectively. The free energy of ITS region gene of T. harzianum secondary structure was – 291.0 kkal/mol. The total restriction enzyme sites of ITS region gene of T. harzianum were 35. The GC and AT content of ITS region gene of T. harzianum were found to be 56 and 44% respectively. These exacting results suggested that fungicide play an important role in controlling the phytopathogenic fungi.

Authors and Affiliations

M. Mahadevi
Kundavai Naachiyaar Government Arts College For Women (Autonomous), Thanjavur,Tamil Nadu, India
A. Thavaselvi
PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, India
V. Latha
Kundavai Naachiyaar Government Arts College For Women (Autonomous), Thanjavur,Tamil Nadu, India
A. Panneerselvam
PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, India

Fungicides, T. harzianum, GC-MS, Biocontrol.

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Publication Details

Published in : Volume 4 | Issue 5 | March-April 2018
Date of Publication : 2018-02-28
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 156-168
Manuscript Number : IJSRST1841274
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

M. Mahadevi, A. Thavaselvi, V. Latha, A. Panneerselvam, " Invitro Screening and Molecular Characterization of Trichoderma Harzianum Against Phytophthora Palmivora (Butl.,) Causing Root Rot Disease in Caricapapaya L.", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 4, Issue 5, pp.156-168, March-April-2018.
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