Plant Growth Promoting Characteristics of Bacillus Licheniformis DS3 Isolated from Agriculture Field Soil

Authors

  • D. Silpa  Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh , India
  • P. Brahmaji Rao  Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh , India
  • G. Kranthi kumar  Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh , India
  • M. Raghu Ram  Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh , India

Keywords:

Ammonia, PGPR, IAA, Gibberellic acid (GA3), Siderophore

Abstract

Plant growth promoting rhizobacteria (PGPR) are known to influence the plant growth by various direct or indirect mechanisms. In search of efficient PGPR strains with multiple activities, a total of 10 bacterial isolates belonging to Bacillus species isolated from different banana field soils, Tadepalli, Guntur district of Andhra Pradesh, India. Out of ten only one strain Bacillus licheniformis DS3 showed potential activity of producing IAA, Gibberellic acid production, Siderophore production and excretion of Ammonia. Bacillus licheniformis DS3 showed the maximum IAA and Gibberellic acid productions are 142 µg/ml and 74.75 µg/ml respectively. An orange halo appears around the Bacillus licheniformis DS3 on CAS agar media indicates the siderophore production. The optimum conditions for IAA production in Bacillus licheniformis DS3 are 0.1 % L-tryptophan, 48 h of incubation, Glucose (1%) and Ammonium sulphate (0.5%) were used as carbon and nitrogen sources. This strain has shown to exhibit multiple plant growth promoting characteristics. These plant growth promoting abilities can make this isolate a potential PGPR candidate for its application in sustainable agriculture.

References

  1. Arshad M., Frankenberger W.T. Jr. 1992. Microbial production of plant growth regulators. In: Metting FB Jr (eds). Soil Microbial Ecol, Marcel Dekker Inc., New York.307-347.
  2. Basu P.S., Ghosh, A.C. 2001. Production of Indole Acetic Acid in cultures by a Rhizobium species from the root nodules of a monocotyledonous tree, Roystonea regia. Acta Biotechnol. 21: 65-72.
  3. Borrow A., P., W, Brain, U., E., Chester, P, .J. Curtis, H., G., Hemming, E., C, Jeffereys,R., B., Lloyd, I., S., Nixon, G., L., F., Norris, and N., Radley. 1955. Gibberellic acids a metabolic product of the fungus Gibberella fujikuroi some observations on its production and isolation. J. Sci. Food. Agric., 6, 340- 348,
  4. Brick J.M., Bostock R.M., Silverstone S. E., 1991. Rapid insitu assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane. Appl. Environ.Microbiol. 57, 535-538.
  5. Cappuccino J. C., Sherman, N., 1992. In: Microbiology: A Laboratory Manual, New York, pp. 125-179.
  6. Gordon S.A. and Weber, R.P. 1951. Colorimetric estimation of indole acetic acid. Plant physiology, 26(1):192-195.
  7. Gutierrez Manero F., J., Ramos, B., Probanza, A., Mehouachi, J., Talon, M. 2001. The plant growth promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiol Plant, 111, 206-211.
  8. Hurek T, Reinhold-Hurek B. Azoarcus spp. strain BH72 as a model for nitrogen fixing grass endophytes. J Biotechnol, 2003:106:169.
  9. Jayaprakashvel M., Abishamala, K., Periasamy, C.M., Satheesh, J., Hussain, A.J. and Vanitha, M.C., 2014. Isolation and Characterization of Indole Acetic Acid (IAA) Produced by a Halo Tolerant Marine Bacterium Isolated from Coastal Sand Dune Plants. Biosciences Biotechnology Research Asia, 11:263-269.
  10. Kayasth M., Kumar, V. and Gera, R., 2013. Exploring the potential of PGPR strain Bacillus licheniformis to be developed as multifunctional biofertilizer. Central European Journal of Biology, 2(1), pp.12-17.
  11. Khalid A., Tahir, S., Arshad, M. and Zahir, Z.A. 2004. Relative efficiency of rhizobacteria for auxin biosynthesis in rhizosphere and non-rhizosphere soils. Aus. J. Soil Res. 42:921-926.
  12. Kloepper JW, Schroth MN. 1978. Plant growth promoting rhizobacteria on radishes. In: Proceedings of IVth International Conference on Plant Pathogenic Bacteria. pp.879-882.
  13. Lee S., Flores, E.M., contreras , Z,M., Gracia , F,L., Escamilla, J,E., and Kennedy,C.2004. IAA biosynthesis is deficient in Gluconacetobacter diazotrophicus strains with mutations in cytochrome c biogenesis gene .Journal of Bacteriology. 186(16): 5384-5391.
  14. Mohite B., 2013. Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of soil science and plant nutrition, 13(3): 638-649.
  15. Patten C.L. and Glick, B.R. 1996. Bacterial biosynthesis of indole-3-acetic acid. Can. J.Microbiol. 42:207-20.
  16. Pattern CL, Glick BR. 2002. Role of Pseudomanas putida indo lactic acid in development of the host plant root system. Applied Environmental Microbiology. 68: 3795-3801.
  17. Schwyn B., Neilands, J.B., 1987. Universal chemical assay for detection and determination of siderophore. Anal. Biochem. 160, 47-56.
  18. Shilts T., Erturk, U., Patel, N.J., Chung, K.R. 2005. Physiological regulation of biosynthesis of Indole-3 -acetic acid and other indole derivatives by the citrus fungal pathogen Collectotrichum acutatum. Journal of Biological Sciences. 5, 205- 210.
  19. Shukla R., Chand, S., Srivastava, A. K. 2005. Batch kinetics and modeling of gibberellic acid production by Gibberella fujikuroi. Enzyme and Microbial Technology.36, 492-7.
  20. Sneath PHA., 1986. Bacillus. In Bergey’s Manual of Systematic Bacteriology, edited by. Mair NS, Sharpe ME, Holt JG, Baltimore, USA, Williams and Wilkins, 2: 1105-1139.

International Journal of Scientific Research in Science and Technology

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Published

2018-02-28

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Volume 4, Issue 2, January-February-2018

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Research Articles

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How to Cite

[1]
D. Silpa, P. Brahmaji Rao, G. Kranthi kumar, M. Raghu Ram, " Plant Growth Promoting Characteristics of Bacillus Licheniformis DS3 Isolated from Agriculture Field Soil, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.1477-1478, January-February-2018.