Angiotensin Converting Enzyme (ACE): Inhibition of Rabbit Lungs ACE in Vitro by Nardostacys Jatamansi

Authors

  • Hinal H Patel  Department of Bioscience, Veer Narmad South Gujarat University, Surat, Gujarat, India
  • Dr. Dhara Gamit  Department of Bioscience, Veer Narmad South Gujarat University, Surat, Gujarat, India

Keywords:

Angiotensin Converting Enzyme, Nardostacys Jatamansi, Vitro, HPTLC, Plant materials

Abstract

In renin-angiotensin-Aldosteron-system, renin produced from juxtaglomerular apparatus of kidney. Produced renin, convert angiotensin to inactive angiotensin-I. With the help of angiotensin converting enzyme, angiotensin-I converted into angiotensin-II which leads to hypertension. In this study methanol, ethyl acetate and petroleum ether extract of Nardostachys jatamansi was used to inhibit ACE. In vitro assay was based on release of hippuric acid from substrate Hippuric-Histidyle-Leucine by ACE and measured spectrophotometrically at 228 nm. ACE from rabbit lungs (sigma Aldrich) was inhibited by methanol, petroleum ether and ethyl acetate extract of Nardostacys jatamansi and higher percentage of inhibition was observed in methanolic extract that confirmed by standard drug inhibitor like Captropril. HPTLC of plant extract was carried out using Toluene: Ethyl acetate, 9.3:0.7solvent system. Presence of different phytoconstituents was observed in HPTLC finger prints. These different phytoconstituents present in the plant help to inhibit ACE. This result support traditional use of plant as antihypertensive.

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International Journal of Scientific Research in Science and Technology

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Published

2018-02-28

Issue

Volume 4, Issue 2, January-February-2018

Section

Research Articles

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How to Cite

[1]
Hinal H Patel, Dr. Dhara Gamit, " Angiotensin Converting Enzyme (ACE): Inhibition of Rabbit Lungs ACE in Vitro by Nardostacys Jatamansi, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.1519-1523, January-February-2018.