Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Sodium and Tizanidine Hydrochloride in Bulk and Tablet Formulation

Authors(3) :-Sonali D Labhade, Sanjay R Chaudhari, Ravi B Saudagar

A reverse phase high performance liquid chromatographic method (RP-HPLC) hasbeen developed for the simultaneous estimation of Diclofenac sodium (DICS), and Tizanidine Hydrochloride (TIZH) in the pharmaceutical formulation. The chromatographic column used was a reverse phase 4.6250 mm Hypersil C18 HPLC column with 5m (particles) packing. The mobile phase was ACN: Phosphate buffer pH 7.0 (50:50 v/v) delivered at a flow rate of 1.0 mL/min. The injection volume was 25 L. Elute was analyzed by a UV detector set at 220 nm .Linearity was obtained inthe concentration range of 2-10 μg/ml for DICS, 2.5- 12.5 μg/ml Tizanidine hydrochloride. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed RP-HPLC method may be used for determining Diclofenac Sodium and Tizanidine Hydrochloride in bulk drug samples.

Authors and Affiliations

Sonali D Labhade
SJVPM's RMD Institute of Pharmaceutical Education and Research, Pune, Maharashtra, India
Sanjay R Chaudhari
SJVPM's RMD Institute of Pharmaceutical Education and Research, Pune, Maharashtra, India
Ravi B Saudagar
KCT's, R G Sapkal College of Pharmacy, Nashik, Maharashtra, India

Diclofenac Sodium, Tizanidine Hydrochloride, High Performance Liquid Chromatography.

  1. Chien, Y.W., 1992. Novel drug delivery system (2nd ed.). Marcel dekker Inc., New York, pp. 139-140.
  2. Chien, Y.W., 1992. Novel drug delivery system (2nd ed.). Marcel dekker Inc., New York, pp. 1-2.
  3. Bankers, G.S., Rhodes, C.T., 1995. Modern pharmaceutics (3rd ed.). Marcel dekker Inc., New York, pp. 575.
  4. Bankers, G.S., Rhodes, C.T., 1995. Modern pharmaceutics (3rd ed.). Marcel dekker Inc., New York, pp. 6.
  5. Bankers, G.S., Rhodes, C.T., 1995. Modern pharmaceutics (3rd ed.). Marcel dekker Inc., New York, pp. 577-578.
  6. Bankers, G.S., Rhodes, C.T., 1995. Modern pharmaceutics (3rd ed.). Marcel dekker Inc., New York, pp. 579-580.
  7. Lachman, L., Liberman, H.A., Kanig J.L., 1987. The theory and practice of industrial pharmacy (3rd ed.). Varghese publishing house, India, pp. 430.
  8. Brahmankar, D.M., Jaiswal, S.B., Biopharmaceutics and pharmacokinetics-a treatise 1st ed. Vallabh prakashan 1995. 347-371.
  9. Lachman, L., Liberman, H.A., Kanig J.L., 1987. The theory and practice of industrial pharmacy (3rd ed.). Varghese publishing house, India, pp. 453.
  10. Lachman, L., Liberman, H.A., Kanig J.L., 1987. The theory and practice of industrial pharmacy (3rd ed.). Varghese publishing house, India, pp. 443-453.
  11. Jantzen, G.M., Robinson, J.R., 1992. Sustained and controlled release drug delivery system, modern pharmaceutics, Modern pharmaceutics, Marcel Dekker, New York, pp. 71-121.
  12. Karande, A., Dhoke, S., Yeole, P., 2006. Formulation and evaluation of bilayer tablet with antihypertensive drugs having different release pattern, Indian Drugs. 43(1), 44-50.
  13. Wang, X.C.F., Yonezawa, Y. Sunada, H., 2003. Preparation and evaluation of combination tablet containing incompatible active ingredients, Chem Pharm Bull. 51, 7772.
  14. Dressman, B., Amidon, G.L. Reppas, C., 1998. Dissolution testing as a prognostic tool for oral drug absorption: immediate release dosage forms, Pharm Res. 15 (1), 11-22.
  15. Subhramanian, G., Musmade, P., Agarwal, S., Udupa, N., 2004. Simultaneous RP-HPLC estimation of tizanidine, diclofenac, paracetamol in tablets, Indian J pharmSci, 694-696.

Publication Details

Published in : Volume 4 | Issue 2 | January-February 2018
Date of Publication : 2018-02-28
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 1413-1417
Manuscript Number : IJSRST184159
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Sonali D Labhade, Sanjay R Chaudhari, Ravi B Saudagar, " Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Sodium and Tizanidine Hydrochloride in Bulk and Tablet Formulation", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 4, Issue 2, pp.1413-1417, January-February-2018.
Journal URL : https://ijsrst.com/IJSRST184159
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