Antibacterial Activity of Marine Fungi Against Veterinary Based Clinical Pathogens

Authors(8) :-G. Anuhya, Ch. Koteswara Rao, G. Samuel, V. Jyostna, B. Bodaiah , C. Uma, I. Karthika Lakshmi, P. Sudhakar

Marine fungal metabolites are bioactive compounds develop and inhibit the growth of microbial and clinical pathogens. So, the Present study was focussed on potentiality of gaps-1 and gaps-2 against standard antibiotic tetracycline by their bioactivity to fetch out the veterinary medicine that are used by the bovine and poultry farmers. Bioactive metabolites were extracted from marine fungal isolates (GAPS-1 and GAPS-2) through solvent extraction. Potency of metabolite was determined by antibacterial assay and MIC (minimum inhibitory concentration) against Clinical pathogens like Escherichia coli, Klebsiella, Pasteurella multocida using standard antibiotic tetracycline as a standard. Fungal extracts of (GAPS-1 and GAPS-2) are effectively extracted in Ethyl acetate, when compared to chloroform, hexane and petroleum either respectively, both extracts had showed a prominence antibacterial activity on all test organisms and MIC of both extracts starts from 500µg where as standard antibiotic tetracycline starts from 250µg. Since both fungal isolates revealed significant effect on clinical pathogens indicating the potentiality of marine environment in producing alternatives of chemically synthesizing standard antibiotics.

Authors and Affiliations

G. Anuhya
Department of Biotechnology, Acharya Nagarjuna University, Andhra Pradesh, India
Ch. Koteswara Rao
Department of Commerce and business Administration, Acharya Nagarjuna University, Andhra Pradesh, India
G. Samuel
Poultry farmer, Dharanikota, Guntur, Andhra Pradesh, India
V. Jyostna
Department of Biotechnology, Acharya Nagarjuna University, Andhra Pradesh, India
B. Bodaiah
Department of Biotechnology, Acharya Nagarjuna University, Andhra Pradesh, India
C. Uma
AD of Department of Epidemiology, Animal Husbandary, Vijayawada, Andhra Pradesh, India
I. Karthika Lakshmi
AD of Department of Epidemiology, Animal Husbandary, Vijayawada, Andhra Pradesh, India
P. Sudhakar
Department of Biotechnology, Acharya Nagarjuna University, Andhra Pradesh, India

Marine fungi, antibacterial assay, Clinical pathogens, MIC, Antibiotics.

  1. J. P. Nataro, C.A. Bopp, P.I. Fields, J. B.; Kaper and N. A. Strockbine. Escherichia, shigella, and salmonella. In Manual of Clinical Microbiology; , J.Versalovic, K.C. Carroll, G. Funke, J.H. Jorgensen, M.L. Landry and D.W Warnock. Eds.; ASM Press: Washington, DC, USA, pp. 603-626, 2011.
  2. M. J. Van den Beld and F. A. Reubsaet. Differentiation between shigella, enteroinvasiveEscherichia coli (EIEC) and noninvasiveEscherichia coli. Eur. J. Clin. Microbiol. Infect. Dis. 31, 899-904, 2012,
  3. F. Qadri, A.I. Khan, A.S. Faruque, Y.A. Begum, F. Chowdhury, G.B. Nair, M.A.Salam, D.A. Sack and A.M. Svennerholm. EnterotoxigenicEscherichia coli and vibrio choleraediarrhea, Bangladesh, 2004. Emerg. Infect. Dis. 11, 1104-1107, 2005.
  4. A. Caprioli, S. Morabito, H. Brugere and E. Oswald Enterohaemorrhagic Escherichia coli: Emerging issues on virulence and modes of transmission. Vet. Res. 36, 289-311, 2005.
  5. J.B. Kaper, J.P. Nataro and H.L. Mobley, Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2, 123-140, 2004.
  6. J. Mehrzad, L. Duchateau, S. Pyorala and C. Burvenich. Blood and milk neutrophils chemiluminescence and viability in primiparous and pluriparous dairy cows during late pregnancy, around parturition and early lactation. J. Dairy Sci. 85, 3268-3276, 2002.
  7. C. Svensson, J. Hultgren and P.A. Oltenacu. Morbidity in 3-7-month-old dairy calves in south-western Sweden, and risk factors for diarrhoea and respiratory disease. Prev Vet Med 74:162-179, 2006.
  8. R.J. Callan and F.B. Garry. Biosecurity and bovine respiratory disease. Vet. Clin. North Am. Food AnimPract 18:57-77, 2002.
  9. J. P. Christensen and M. Bisgaard. Fowl cholera. Rev Sci Tech OffIntEpiz. 19:626-637, 2000. PubMed].
  10. R. B. Rimler and J. R. Glisson. Fowl cholera. In: B. W. Calnek, H. J. Barnes, C. W. Beard, L. R. McDougald and Y. M. Saif. editors. Diseases of poultry. 10th ed. Ames, Iowa: Iowa state University press; pp. 143-161, 1997.
  11. M. Bisgaard Ecology and significance of Pasteurellaceae in animals. ZentblBakteriol. 279:7-26, 1993.PubMed],
  12. W. Frederiksen Ecology and significance of Pasteurellaceae in man—an update. ZentblBakteriol. 279:27-34, 1993. PubMed]
  13. S. Thanigaivel, S. Hindu Vidhya, S. Vijayakumar, A. Mukherjee, N. Chandrasekaran and J. Thomas. Differential solvent extraction of two seaweeds and their efficacy in controlling Aeromonassalmonicida infection in Oreochromismossambicus a novel therapeutic approach. Aquaculture 433, 56-64, 2015.
  14. K. Jalil, R. Vadood and B. Abolfazi. Isolation of Escherichia coli O157:H7 from manure fertilized farms and raw vegetables grown on it, in Tabriz city in Iran. Afr. J. Microbiol. Res. 4, 891-895, 2010.
  15. NCCLS- National Committee for Clinical Laboratory Standards: Performance standards for antimicrobial disc susceptibility tests. 8th edition. Approved Standard, document M2-A8SNCCLS, Wayne, Pennsylvania; 2003.
  16. A. Saha and M. S. Rahman Antimicrobial Activity of Crude Extract from Calycopterisfloribunsa. Bang. J. Microbiol. 25:137-139, 2008

Publication Details

Published in : Volume 4 | Issue 5 | March-April 2018
Date of Publication : 2018-04-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 742-746
Manuscript Number : IJSRST1845141
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

G. Anuhya, Ch. Koteswara Rao, G. Samuel, V. Jyostna, B. Bodaiah , C. Uma, I. Karthika Lakshmi, P. Sudhakar, " Antibacterial Activity of Marine Fungi Against Veterinary Based Clinical Pathogens ", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 4, Issue 5, pp.742-746, March-April-2018.
Journal URL : https://ijsrst.com/IJSRST1845141
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