Preliminary Characterization of Rhizobacterial Strains Isolated from Legume [Vigna trilobata (L.) verdc.] Root Nodules

Authors(2) :-G. Kranthi Kumar, M. Raghu Ram

Four Rhizobial strains were isolated from root nodules of Vigna trilobata (L –Verdc) plants raised in soils of different areas in Andhra Pradesh India. These strains were isolated on selective Yeast Extract Mannitol Agar (YEMA) medium. In the present study four Rhizobial strains were further subjected to physiochemical and biochemical characteristics. The Rhizobium strains were rod shaped gram negative and mucilaginous in nature. Except Rhizobium sp. MRR106 all the isolated colonies showed in white colour. Further these four strains were observed for physical conditions like pH, NaCl and temperature for their growth. Optimum conditions of these strains were at 30? C temperatures, 0.1% NaCl and a neutral pH 7.0. All the four strains were positive for biochemical tests such as ammonia production test, oxidase test, catalase test, nitrate reductase test and urease test. Except Rhizobium sp. MRR 123 all the strains showed amylase and citrate utilization tests. All these strains showed negative chemical reaction on hofer’s alkaline broth test, Ketolactose test and glucose peptone agar tests.

Authors and Affiliations

G. Kranthi Kumar
Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, India
M. Raghu Ram
Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, India

Rhizobium, Vigna trilobata, Yeast Extract Mannitol Agar

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Publication Details

Published in : Volume 4 | Issue 7 | March-April 2018
Date of Publication : 2018-04-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 279-284
Manuscript Number : IJSRST1845247
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

G. Kranthi Kumar, M. Raghu Ram, " Preliminary Characterization of Rhizobacterial Strains Isolated from Legume [Vigna trilobata (L.) verdc.] Root Nodules", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 4, Issue 7, pp.279-284, March-April-2018.
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