Development and Validation of Stability Indicating Ultra Performance Liquid Chromatography method for simultaneous Quantification of Thiophante-methyl, Fipronil and Pyraclostrobin in Pesticide Formulation

Authors

  • Dilip K. Patel  School of Basic and Applied Science, Raffles University, Neemrana Alwar, Rajasthan, India
  • Babu Lal Swami  School of Basic and Applied Science, Raffles University, Neemrana Alwar, Rajasthan, India
  • T. L. Rajawat  School of Basic and Applied Science, Raffles University, Neemrana Alwar, Rajasthan, India

Keywords:

Thiophante-methyl; Fipronil; Pyraclostrobin; Stability indicating; Validation; Horwitz equation; FS-Flow-able concentrate for Seed treatment, CIPAC - Collaborative International Pesticides Analytical council, Uncertainty in measurements.

Abstract

A novel stability-indicating ultra-performance liquid chromatography (UPLC) method has been developed and validated for quantification of Thiophanate-methyl, Fipronil and Pyraclostrobin in pesticide formulation (FS), using Poroshell 120 EC-C18 (100 mm × 4.6 mm, 2.7µm) column. Mixture of 0.1% ortho-phosphoric acid: acetonitrile (40:60 v/v) was used as mobile phase. The flow rate was kept 0.75 ml/min and detection was carried out at 275 nm. The limit of detection was 0.00017 mg/ml, 0.0010 mg/ml and 0.00022 mg/ml for Thiophanate-methyl, Fipronil and Pyraclostrobin respectively. The limit of quantitation values was 0.00035mg/ml, 0.0020mg/ml and 0.00035mg/ml for Thiophanate-methyl, Fipronil and Pyraclostrobin respectively. The linearity of proposed method was investigated in the range of 0.00038-0.661mg/ml (r2=0.9993), 0.00202-0.743mg/ml (r2=0.9997) and 0.0004-0.091mg/ml (r2=0.9996) for Thiophanate-methyl, Fipronil and Pyraclostrobin respectively. The percentage recovery found to be in range from 98.4-100.0 %, 98.4-99.1% and 98.5-99.3% for Thiophanate-methyl, Fipronil and Pyraclostrobin respectively. The % RSD values for intraday precision study and inter-day precision study were <1.65, <1.68 and <2.33 for Thiophanate-methyl, Fipronil and Pyraclostrobin respectively as per modified Horwitz equation as requirements by CIPAC. The developed method was found to be specific, linear, precise, accurate and robust. This method is also useful for quantification of Thiophanate-methyl, Fipronil and Pyraclostrobin in their single or combination formulated products, environmental samples (soil, water), and agricultural products for pesticide residue analysis.

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International Journal of Scientific Research in Science and Technology

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Published

2018-04-30

Issue

Volume 4, Issue 5, March-April-2018

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Research Articles

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How to Cite

[1]
Dilip K. Patel, Babu Lal Swami, T. L. Rajawat, " Development and Validation of Stability Indicating Ultra Performance Liquid Chromatography method for simultaneous Quantification of Thiophante-methyl, Fipronil and Pyraclostrobin in Pesticide Formulation, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 5, pp.1308-1317, March-April-2018.