Modulating effects of Piper Nigrum Fruit extract Against Adriamycin Induced Genotoxicity in Swiss Albino Mice

Abstract

In the present research, studies were carried out to examine the modulating effects of crude Piper nigrum fruit extract against Adriamycin induced genotoxicity in germ cells of Swiss Albino mice using Sperm Morphology Assay. Two experiments were conducted using three different doses 200, 300, 400 mg/kg body weight of the crude Piper nigrum fruit extract and 16mg/kg body weight Adriamycin in germ cells of mice. In first test the antigenotoxic effects of Piper nigrum extract was studied and in the second test one group of animals were treated with Adriamycin alone and other groups of animals were primed with crude Piper nigrum fruit extract prior to adriamycin treatment . Control animals were fed only with physiological saline. All the exposed and control animals were sacrificed on last day of fifth week and cauda epididymis was dissected out, smeared, stained and screened for the presence of sperm abnormalities in all control and treated animal groups. The results were found to be insignificant in crude Piper nigrum fruit extract treated animal groups. A significant increase in the percentage of abnormal sperms were noted when treated with Adriamycin alone and the cells showed inhibition in the percentage of abnormal sperms when animals were primed with crude Piper nigrum fruit extract prior to adriamycin treatment. The present study reveals that the crude Piper nigrum fruit extract nonmutagenic nature. Hence crude Piper nigrum fruit extract supplementation is safer in chemotherapeutic strategy.