Analytical Method Development and Validation for The Simultaneous Estimation of Azithromycin and Cefixime by Rp-Hplc Method in Bulk and Pharmaceutical Formulations

Authors

  • K. Nagaraju  Research scholar, Krishna university, Machilipatnam , Krishna, Andhra Pradesh, India
  • Y. A. Chowdary  Sir C. R Reddy college of Pharmaceutical Sciences, Eluru, W.G 2NRI College of Pharmacy, Pothavarappadu (V), Via Nunna,Agiripalli (M), Vijayawada Rural, Krishna, Andhra Pradesh, India

Keywords:

Azithomycin and Cefixime,RP-HPLC

Abstract

To develop and validate a simple, rapid, accurate and precise RP-HPLC method for the simultaneous determination of azithromycin and cefixime in bulk and pharmaceutical formulation. Chromatographic separation was performed on a supleco C18 (25cm×4.6 mm, 5 µm) column from thermo isocratic mode with mobile phase 80:20 Na2HPO4: Methanol with pH adjusted to 8 with methanol at flow rate 1 ml/min. Peak intensity of both the drugs was monitored at 273 nm with PDA detection.The retention time (RT) of Azithomycin and Cefixime was found to be 2.77 and 4.93 min, respectively. The linearity of Azithromycin and Cefixime were found in the range of 50-150 µg/ml . The limit of detection and limit of quantitation was3mg/ml and 10 mg/ml for Azithromycin and 3 mg/ml and 10 mg/ml for CHL.The proposed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantification. Furthermore, no interference was observed with extra pharmacopoeial excipients in tablet suggesting its utility for routine quality control analysis of Azithromycin and Cefixime in pharmaceutical formulations

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International Journal of Scientific Research in Science and Technology

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Published

2018-04-30

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Volume 4, Issue 5, March-April-2018

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Research Articles

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How to Cite

[1]
K. Nagaraju, Y. A. Chowdary, " Analytical Method Development and Validation for The Simultaneous Estimation of Azithromycin and Cefixime by Rp-Hplc Method in Bulk and Pharmaceutical Formulations, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 5, pp.669-674, March-April-2018.