In-silico Homology Modeling of MMP25 involved in Asthma

Authors

  • Yogesh N. Joshi  Department of PG Studies and Research in Bioinformatics, Walchand Centre for Biotechnology, Walchand College of Arts & Science, Solapur, Maharashtra, India
  • Shruti G. Gajul  Department of PG Studies and Research in Bioinformatics, Walchand Centre for Biotechnology, Walchand College of Arts & Science, Solapur, Maharashtra, India

Keywords:

In-Silico, Asthma, MMP25, UniProtKB, Homology Modeling

Abstract

Asthma is a chronic long term inflammatory disease of the airways of lungs in which the airways narrow, swell and produce extra mucus. This leads to a limitation of the flow of air to and from the lungs, causing shortness of breathing. According to the latest WHO estimates released in December 2016, there were 3,97,000 deaths in 2015 and about 334 million patients are suffering from asthma across the world. It is caused by a combination of genetic and environment factors. Exposure to second hand smoke, exhaust fumes or other types of allergens is the major risk factors for developing asthma. Subsequent work suggest that the MMP25 protein plays a crucial role for causing airway remodeling in asthma disease by cleaving fibronectin, a extracellular component secreted by fibroblast which maintains the form, solidity and integrity of the lungs. In the present study, we used different In-Silico tools and techniques which includes retrieval of MMP25 protein sequence from UniProt KB database and the sequence analysis was performed by using Protparam tool which concluded that the protein was unstable and basic in nature. The secondary structure was predicted by using GOR tool which indicated that the percentage of coils was higher than the percentage of alpha helix and extended strand. Then the 3D structure of MMP25 was predicted by using SWISS MODEL server and the model was validated by using PROCHECK analysis tool. After validation of the model, the validation score was 89.40% indicating that the model was of good quality and the predicted 3D structure was deposited in protein model database (PMDB). Further study on MMP25 protein can be carried out in molecular docking and in structure based drug designing (SBDD) to inhibit airway remodeling in asthma.

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International Journal of Scientific Research in Science and Technology

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Published

2018-07-30

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Volume 4, Issue 9, July-August-2018

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Research Articles

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How to Cite

[1]
Yogesh N. Joshi, Shruti G. Gajul, " In-silico Homology Modeling of MMP25 involved in Asthma, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 9, pp.202-208, July-August-2018.