Effects of Quinalphos on Glycogen and Protein Content in Different Tissues of the Freshwater Fish, Channa Gachua (Hamilton, 1822)

Authors(2) :-Nilesh B. Pakhare, Sidharth D. Pagare

This study was conducted to investigate changes in the glycogen and protein content of the gill, liver, muscle and kidney of the air-breathing fish Channa gachua exposed to chronic exposure of quinalphos for 10, 20 and 30 days exposure at 1/10th of 96 hr toxicity (0.147 ppm). The estimated glycogen in the tissue gill, liver, muscle and kidney were found to reduce during the exposure period, the liver was most affected than the other tissues in compare to control. The disruption of enzyme associated with carbohydrate metabolism resulted in glycogen depletion. The estimated protein in the tissue gill, liver, kidney and muscle were found to significantly reduce during the exposure period. Protein degradation in the tissues of test fish suggests that the quinalphos disturbing the process of protein metabolism by declining the protein level due to the disturbance in the proteolysis.

Authors and Affiliations

Nilesh B. Pakhare
Department of Zoology, Deogiri college, Railway Station Road, Aurangabad, Maharashtra, India
Sidharth D. Pagare
Department of Zoology, K. A. A. N. M. Sonawane Arts, Commerce and Science College, Satana Dist. Nashik, Maharashtra, India

Channa gachua, Gill, Glycogen, Kidney, Liver, Muscle, Protein, Quinalphos

  1. Barbieri E., (2008): Effect of 2,4-D herbicide (2,4-Dichlorophenoxyacetic acid) on oxygen consumption and ammonium excretion of juveniles of Geophagus brasiliensis (Quoy and Gaimard, 1824) (Osteichtyes, Cichlidae). Ecotoxicology.
  2. Baruah, B. K., Sengupta, S. and Das, M. (2004): Effect of Paper mill effluent of muscle protein profile of fish Heteropneustes fossilis (Bloch). Poll. Res. 23(4): 623-625.
  3. Binukumari, S. and Vasanthi, J. (2013): Toxicity of the pesticide dimethoate 30 % EC on the carbohydrate content of the freshwater fish, Labeo rohita. J. of Chem., Biol. and Phys. Sci., 4(1): 220-223.
  4. Calvert G. M., Sanderson W. T., Barnett M., Blondell J. M. and Melher L. N., (2001). Surveillance of pesticide-related illness and injury in humans. In R. Krieger et al., (Eds). Handbook of pesticide toxicology, 2nd edn. (pp. 603-641). New York, NY: Academic.
  5. Chebbi S. G. and David M., (2009): Neurobehavioral responses of the freshwater teleost, Cyprinus carpio (Linnaeus.) under Quinalphos intoxication. Biotechnology in animal husbandry. 25(3-4): 241-249.
  6. Das B. K. and Mukherjee S. C., (2000): Sublethal effects of Quinalphos on selected blood parameters of Labeo rohita (Ham) fingerlings. Asian fish. Sci., 13: 225-233.
  7. Dautremepuits C., Paris-Palacios S., Betoulle S. and Vernet G., (2004): Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan. Comparative Biochemistry and physiology part C: Toxicology and pharmacology 137: 325-333.
  8. Dezwaan, A. and Zandee, D. T. (1972): The utilization of glycogen accumulation of some intermediates during anaerobiosis in Mytilus edulis L. Comp. Biochem. Physiol., B. 43: 47-54.
  9. Ganeshwade R. M., Rokade P. B. and Sonwane S. R., (2012): Impact of Dimethoate on protein contents in the freshwater fish Puntius ticto (Ham). The Bioscan. 7(1): 153-155.
  10. Harper’s Illustrated Biochemistry (27th Edn.) Robert K. Murray, Daryl K. Garnner adn W. Victor Rodwell (2006): Lange Medical Publications. Asia, Singapore. 74-75.
  11. Jain S. (2014): Sub-lethal effects of methyl parathion on protein content of different tissues of Channa gachua. Int. J. of Life Sci. 2(2): 114-118.
  12. Lakshmanan S., Rajendran A. and Sivasubramaniyan C., (2013): Impact of Dichlorvos on tissue glycogen and protein content in freshwater fingerlings, Oreochromis mossambicus (Peters). Inter. J. Res. Environ. Sci. and Tech. 3(1): 19-25.
  13. Lehninger Principles of Biochemistry (5th Edn.) Michael M. Cox and David L. Nelson (2008): New York. pp. 570-572.
  14. Lopes P. A., Pinheiro T., Santos M. C., Da Loz Mathias B. M., Collares-Pereira M. J. and Viegas-Crespo A. M., (2001): Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Sci. of the Total Environ. 280: 153-163.
  15. Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. J. (1951): Protein measurement with folin phenol reagent. J. Biol. Chem., 193: 265-275.
  16. Magar R. S. and Shaikh A., (2012): Biochemical changes in proteins and amino acids in Channa punctatus in response to sub-lethal treatment with the insecticide malathion. Trends in Life Sciences. 1(3): 19-23.
  17. Marigoudar S. R., (2012): Cypermethrin induced pathophysiological and some biochemical changes in the freshwater teleost, Labeo rohita (Hamilton). PhD thesis submitted to Karnatak University, Dharwad. 110-111.
  18. Mary Chandravathy and Reddy (1996): Lead nitrate exposure changes in carbohydrate metabolism of freshwater fish. J. Environ. Biol. 16(1):75-79.
  19. Mishra A. and Devi Y., (2014): Histopathological alterations in the brain (optic tectum) of the fresh water teleost Channa punctatus in response to acute and subchronic exposure to the pesticide chlorpyrifos. Acta. Histochem. 116:176–81.
  20. Muttappa K., Reddy H. R. V., Rajesh M. and Padmanabha A. (2014): Quinalphos induced alteration in respiratory rate and food consumption of freshwater fish Cyprinus carpio. Journal of Environ. Bio. 35: 395-398.
  21. Pakhare, N. B. and Reddy, K. R., (2017): Efficacy of Quinalphos (25% EC) on Protein content in different tissues of the freshwater fish, Channa gachua (Hamilton, 1822). IJRASET. 5(12): 987-991.
  22. Pakhare, N. B., Gaikwad, S. B. and Reddy, K. R., (2016): Acute Toxicity of Quinalphos (25% EC) on Glycogen content in different tissues of the freshwater fish, Channa gachua (Hamilton, 1822). The Ecoscan. Vol 9: 783-787.
  23. Palanivelu V., Vijayavel K., Balasubramanian S. and Balasubramanian M. P., (2005): Influence of insecticidal derivative (Cartap hydrochloride) from the marine polycheate on certain enzyme systems of the freshwater fish Oreochromis mossambicus. J. Environ. Biol. 26: 191-195.
  24. Ram S. V. (2016): Indoxacarb (AVAUNT 14.5% SC) an insecticide induced toxicity biochemical and histopathological changes in the freshwater murrel Channa Punctatus (Bloch). PhD thesis, Acharya Nagarjuna University.
  25. Sastry, K. V. and Siddiqui, A. A. (1984): Some haematological, biochemical and enzymological parameters of a freshwater teleost fish, Channa punctatus, exposed to sublethal concentrations of quinalphos. Pestic. Biochem. Physiol. 22: 8-13.
  26. Satyavardhan K., (2013): Effect of fenvalerateTM and malathionTM on biochemical constituents of freshwater fish, Ctenopharyngodon idella (Valenciennes). World Appl. Sci. J. 27(5): 649-655.
  27. Schmidt N. B., (1975): Osmoregulation: Effect of salinity and heavy metal. Fed. Proc., 33: 2137-2146.
  28. Shobha, K., A. Poornima, P. Harini and Veeraiah, K. (2007): A study on biochemical changes in the freshwater fish, Catla catla (Hamilton) exposed to the heavy metal toxicant cadmium chloride. Kathmandu Univ. J. Sci. Eng. Technol., 1: 1-11.
  29. Snodgrass W. R. (2001). Diagnosis and treatment of poisoning due to pesticides. In R. Krieger et al., (Eds). Handbook of pesticide toxicology, 2nd edn. (pp. 589-602). New York, NY: Academic.
  30. Somaiah, K., Satish, P. V. V., Sunita, K., Nagaraju, B. and Oyebola, O. O., (2014): Toxic impact of phenthoate on protein and glycogen levels in certain tissues of Indian major carp Labeo rohita (Hamilton). IOSR J. of Envi. Sci., Tox. and Food Tech., 8(9): 2319-2399.
  31. Stalin I. S. and Das S. M. S., (2012): Biochemical changes in certain tissues of Cirrhina mrigala (Hamilton) (Cyprinidae: Cyprniformes) exposed to fenthion. Inter. J. Environ. Sci. 2(3): 1268-1277.
  32. Swarna Kumari, R., Vijaya Kumar, M., and Tilak, K. S. (2008): Biochemical changes of total proteins and glycogen in the tissues of grass carp Ctenopharyngodon idella (Valenciennes), exposed to an organo phosphate Nuvan (76% Ec). J. Aqua. Biol. 23(1):159- 168.
  33. UNEP. (2005): Ridding the world of Persistent Organic Pollutants: A guide to the Stockholm convention on persistent organic pollutants. United Nations Environment Programme, 11-13, Chemin des Anemone, CH-1219, Chatelaine, Geneva, Switzerland, p. 24.
  34. Venkateswara Rao, J., (2006): Sublethal effects of an organophosphorus insecticide (RPR-ll) on biochemical parameters of tilapia, Oreochromis mossambicus. Copm. Biochem. Phy. 143 (4):492-498.
  35. Vornanen, M., J. Asikainen and J. Haverinen, (2011): Body mass dependence of glycogen stores in the anoxia-tolerant crucian carp (Carassius carassius L.). Naturwissenschaften, 98: 225-232.

Publication Details

Published in : Volume 4 | Issue 9 | July-August 2018
Date of Publication : 2018-08-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 314-320
Manuscript Number : IJSRST184978
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Nilesh B. Pakhare, Sidharth D. Pagare, " Effects of Quinalphos on Glycogen and Protein Content in Different Tissues of the Freshwater Fish, Channa Gachua (Hamilton, 1822)", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 4, Issue 9, pp.314-320, July-August-2018.
Journal URL : http://ijsrst.com/IJSRST184978

Article Preview