Protein, Ascorbic Acid and Antioxidative Enzymes Alterations In The Digestive Gland of Lamellidenscorrianus Due to Heavy Metals from Different Reservoirs of Nashik District. (M.S.)

Authors

  • Rahane Balasaheb  Department of Zoology, Swami Muktanand College of Science, Yeola, Dist. Nasik, Maharashtra, India
  • Bhalla Resham  Department of Zoology, LVH Arts, Science & Commerce College, Panchavati, Nashik, Maharashtra, India

Keywords:

Lamellidenscorrianus,heavy metals, proteins, ascorbic acid, antioxidant enzymes.

Abstract

The heavy metals Zn, Cu, Pb and Cd concentrations were determined in surface water and the freshwater bivalve lamellidens corrianus were collected from Girna, Ozarkhed, Chankapur and Gangapur reservoirs of Nasik district during summer, monsoon and winter seasons.The biochemical components proteins, ascorbic acid and oxidative stress indicator parameters like activity of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione-S-transferase (GST), the levels of antioxidant scavenger molecules, reduced glutathione(GSH) and lipid peroxidation (LPO) were estimated from digestive glands of the freshwater bivalve lamellidenscorrianus.The results demonstrate that the level of proteins, ascorbic acid, LPO and activity of GST were lowest and activity of antioxidant enzyme CAT, GPx and SOD were highest at Gangapur reservoir and lowest at Girna reservoir. The results also indicates that the level of LPO and activity of GST were lowest and CAT, GPxand SOD activity were highest in monsoon, while level of LPO and activity of GST were highest and CAT, GPx and SOD activity were lowest in summer season at four reservoirs in digestive glands oflamellidenscorrianus. The mean values of heavy metals Zn, Cu, Pb and Cd concentrations in surface water were highest at Girna reservoir and lowest at Gangapur reservoir. Therefore, it was concluded that Girna reservoir was more polluted than other studied reservoirs.

References

  1. Aebi,H. (1984):Catalase invitro. Methods in Enzymol. 105,121-126.
  2. Banni M,Bouraoui Z,Ghedira J,Clerandeau C,Guerbej H,Narbonne JF,Boussetta H,(2008) :Acute effects of benzoa]pyrene on liver phase I and II enzymes,and DNA damage on sea bream Sparus aurata. Fish Physiol Biochem 34:201–207.
  3. Barron MG (2003): Bioaccumulation and bioconcentration in aquatic organisms. In: Handbook of exotoxicology (2nd edition),(Eds.) Hoffman,DJ Rattner,BA Burton,GA (Jr.) and Cairns J (Jr.) Lewis Publishers,Boacan Raton: 877-888.
  4. Beauchamp,C and Fridovich,I (1973): Isozymes of superoxide dismutase from wheat germ. Biochimica et Biophysica Acta,317: 50-64.
  5. Bocchetti R,Fattorini D,Pisanelli B,Macchia S,Oliviero L,Pilato F,Pellegrini D,Regoli F (2008):Contaminant accumulation and biomarker responses in caged mussels,Mytilus galloprovincialis,to evaluate bioavailability and toxicological effects of remobilized chemicals during dredging and disposal operations in harbor areas. Aquat Toxicol 89:257–266.
  6. Bouraoui Z,Banni M,Ghedira J,Clerandeau C,Narbonne JF,Boussetta H. (2009): Evaluation of enzymaticbiomarkers and lipoperoxidation level in Hediste diversicolor exposed to copperand benzoa]pyrene. Ecotoxicol Environ Saf; 72:1893–8.
  7. Box,A.,Sureda,A.,Galgani,F.,Pons,A.,& Deudero,S. (2007):Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis. Comparative Biochemistry and Physiology,Part C 146: 531-539.
  8. Boyne,A.F. and G.L. Ellman,(1972): A methodology for analysis of tissue sulfhydryl components. Anal. Biochem.,46: 639-653.
  9. Canesi,L.,Borghi,C.,Ciacci,C.,Fabbri,R.,Lorusso,L.C.,Vergani,L.,Marcomini,A.,Poiana,G.,(2008):ShorttermeffectsofenvironmentallyrelevantconcentrationsofEDCmixtureson Mytilus galloprovincialis digestive gland.Aquat. Toxicol.87,272–279.
  10. Cargnelutti D,Tabaldi LA,Spanevello RM,Jucoski GO,Battisti V,Redin M,Linares CEB,Dressler VL,Flores EMM,Nicoloso FT,Morsch VM,Schetinger MRC,(2006) :Mercury toxicity induces oxidative stress in growing cucumber seedlings. Chemosphere 65:999-1006.
  11. Dafre A.L,Medeiros I.D,Muller I.C,Ventura E.C,Bainy A.C.D. (2004):Antioxidantenzymes and thiol/disulfide status in the digestive gland of the brownmussel Perna perna exposed to lead and paraquat. Chem Biol Interact;149:97 – 105.
  12. Deshmukh G.M.(2013): Biomonitoring of heavy metal pollution of jayakwadi reservoir at Paithan by using bivalves as bioindicators. Ph.D.thesis submitted to Dr.B.A.M.University,Aurangabad,(M.S.) India.
  13. Gamble.S,Goldfarb,P.S.,Porte,C.,Livingstone,D.R. (1995): Glutathione peroxidase and other antioxidant enzyme function in marine invertebrates (Mytilus eulis,Pecten maximus,Carcinus maenas and Asterias rubens) Mar.Environ.Res.39,191-195.
  14. Grune,T. (2000): Oxidative stress,aging and the proteasomal system. Biogerontology. 1,31-40.
  15. Habig,W.J.,Babst,M.J. and Jacoby,W.J. (1974) :Glutathione s-transferase the first step in mercapturic acid formation. JBC.,249,7130.
  16. Houston,D.K. and M.A. Johnson,(2000): Does Vitamin C intake protect against lead toxicity? Nutr. Rev.,58(3): 73-75.
  17. (Iratoet.a. 2003). Irato,P.,Santovito,G.,Cassini,A.,Piccinni,E. and Albergoni,V. (2003) :Metal accumulation and binding protein induction in Mytilus galloprovincialis,Scapharca inaequivalvis,and Tapes philippinarum from the Lagoon of Venice. Archives of Environmental Contamination and Toxicology, 44: 476-484.
  18. Iwana,G.K.,Thomas,P.T.,Forsyth,R.B. and Vijayan,M.M.,(1998): Heat shock proteinexpression in fish,reviews in fish Biol-Fisheries. 8(1):35-56.
  19. Kohler,T.,Van Delden,C.,Curty,L.K.,Hamzehpour,M.M.,and Pechere,J.C. (2001) :Overexpression of the MexEF-OprN multidrug efflux system affects cell-to-cell signaling in Pseudomonas aeruginosa. J Bacteriol 183: 5213–5222.
  20. Lodhi H.S.,Khan M.A.,Verma R.S. and Sharma U.D. (2006): Acute toxicity of copper sulphate to fresh water prawns. J. Environ. Biol.,27(3),585-588.
  21. Lowry O.M,Rosenbroughty,N.J,Farr A.L,Randall,R.F (1951):Protein estimation with folin phenol reagent. J. Biol. Chem.,193: 265-275.
  22. Medeiros,R.J.,dos Santos,L.M.G.,Freire,A.S.,Santelli,R.E.,Braga,A.M.C.B.,Krauss,T.M.,and Jacob,S.D.(2012): Determination of inorganic trace elements in edible marine fish from Rio de Janeiro State,Brazil. Food Control 23:535-541.
  23. Nandi,D.,Patra,R.C. and Swarup,D. (2005): Effect of cysteine,methionine,ascorbic acid and thiamine on arsenic-induced oxidative stress and biochemical alterations in rats. Toxicology. 211:226–235.
  24. Nawale,S.P. (2008): Synergistic effect of Caffeine (1,3,7- Trymethylxanthine) and ascorbic acid on heavy metal induced alterations in an experimental model,Lamellidens corrianus (Lea). Ph. D. Thesis,Dr. Babasaheb Ambedkar Marathwada University,Aurangabad (M.S.) India.
  25. Nedjoud,G.,Houria,B.,Rachid,R.,Amira,A. andReda,D.M. (2009): “Impact of Pollution by Industrial Metallic Dust on Bioaccumulator Organism Helix Aspersa”,Global Veterinaria,Vol. 3,No. 4:276-280.
  26. Osman,A.,Heuvel,H.,and Noort,P. (2007): Differential responses of biomarkers in tissues of a freshwater mussel,Dreissena polymorpha,to the exposure of sediment extracts with different levels of contamination. Journal ofApplied Toxicology. 27: 51-59.
  27. Otitoloju,A.A. and Don-Pedro,K.N. (2004): Integrated laboratory and field assessments of heavy metal accumulation in edible periwinkle,Tympanotonus fuscatus Var. radula (L.). Ecotoxicol. Environ. Safety,57: 354-362.
  28. Pavlovic SZ,Belic D,Blagojevic DP,Radojicic MR,Zikic VR,Saicic SZ,Grubor-Lajsic G and Spasic MB (2004): Seasonal variations of cytosolic antioxidant enzyme activities in the liver and white muscle of thin lip gray mullet (Liza ramada Risso) from the Adriatic sea. Cryo Letters,25: 273-285.
  29. Pipe,R. K.,Coles,J. A.,Carissan,F. M. M. and Ramanathan,K. (1999):Copperinduced immunomodulation in the marine mussel Mytilus edulis.Aquat.Toxicol.46,43-54.
  30. Pottinger,T.G.; Carrick,T.R.; Yeomans,W.E. (2002):The three-spined stickleback as an environmental sentinel: effects of stressors on whole-body physiological indices. J Fish Biol. 61: 207-229.
  31. Rajkumar,J.S.I. and Milton,M.C.J. (2011) : Biochemical markers of oxidative stress in Mugil cephalus exposed to cadmium,copper,lead and zinc. Int. J. Pharm. Biosci.,2: 41-50.
  32. Rao M.V.,Chinoy N.J.,Suthar M.B. and Rajvanshi M.I.(2001):Role of ascorbic acid on mercuric chloride-induced genotoxicity in human blood cultures. Toxicol In Vitro 15:649-654.
  33. Roe J. H. (1967): Method of Biochemical analysis Vol. 5,(Eds.By GlickInterscience,New York),5: 44-45.
  34. RotruckJ.T,Pope A.L,Ganther H.E,Swanson A.B,Hafeman D.G,and Hoekstra W.G,(1973): Science,179:588-590.
  35. Sarabjeet Singh Ahluwalia & Dinesh Goyal,(2007): “Microbial and plant derived biomass for removal of heavy metals from wastewater”,Bio resource Technology,(12):2243-2257.
  36. Valavanidis,A.,Vlahogianni,T.,Dassenakis,M.,Scoullos,M.,(2006): Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicol. Environ. Safe. 46,178–189.
  37. Vasseur,P.,Cossu-Leguille,C.,(2003): Biomarkers and communityindices as complementary tools for environmental safety. EnvironmentInternational 28,711–717.
  38. Verlecar,X.N.,Jena,K.B.,Chainy,G.B.N.,(2008): Modulation of antioxidant defences in digestive gland of Perna viridis (L.),on mercury exposures. Chemosphere 71,1977–1985.
  39. Vincent S.,Ambhore T.,Kumar L. C. A. and Selvanayagam M. (1995): Biochemical response of the Indian major carp,Catlacatla (Ham) to Chromium toxicity. Indian J. Environ. Health. 37(3): 190-196.
  40. Waykar,B. and Lomte,V. S. (2001): Total protein alteration in different tissues of fresh water bivalve,Parreysia cylindrica after cypermethrin exposure. Ecol. Env.and Cons.7(4): 465-469.
  41. Waykar,B. and Shinde,S. (2011): Assessment of the heavy metal bioaccumulation in three species of fresh water bivalves. Bull. Env.Contam.Toxico. 87(3): 267-271.
  42. Yigit and Altindag,(2006):Yigit,Sibel and Ahmet,Altindag. (2006): Concentration of heavy metals in the food web of Lake Egirdir,Turkey. J Environmental Biology,27: 475-478.
  43. Zhang,M.,Zhao,Z.,He,L.and Wan C. A. (2010): Meta-analysis of oxidative stress markers in schizophrenia. Sci China LifeSci 53: 112-24.
  44. Zhou,Q.,Zhang,J.,Fu,J.,Shi,J. and Jiang,G.(2008):Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem. Anal.Chim.Acta,606: 135-150.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2018-01-20

Issue

Volume 5, Issue 1, January-February-2018

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Rahane Balasaheb, Bhalla Resham, " Protein, Ascorbic Acid and Antioxidative Enzymes Alterations In The Digestive Gland of Lamellidenscorrianus Due to Heavy Metals from Different Reservoirs of Nashik District. (M.S.), International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 5, Issue 1, pp.30-37, January-February-2018.