In Vitro Response of GA3 in Caulogenesis of Fiver nut

Authors(2) :-Manju Rakesh, Patil N. M.

A protocol was optimized for the caulogenesis of fiver nut. Internodal explant showed immediate response in shoot regeneration and production of callus in in vitro cultures of Caesalpiniabonducella (L.) Roxb.commonly known as fiver nut. MS medium supplemented with 1 to 10 mg/l GA 3 was found to induce callus. The Internodal explant inoculated on MS medium with 6 mg/l GA3 was found to produce shoots after 35 days of inoculation. Maximum amount of pale yellow coloured friable callus was produced in 7mg/l GA3 of dry weight 1.513± 0.108 g. The method can be used to generate callus and shoot which are natural sources of pharmaceutical compounds without disturbing the natural population of the plant.

Authors and Affiliations

Manju Rakesh
Post Graduate Research Center, Modern College, Shivajinagar, Pune, Maharashtra, India
Patil N. M.
Post Graduate Research Center, Modern College, Shivajinagar, Pune, Maharashtra, India

Fiver Nut, Caulogenesis , Callus.

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Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 128-131
Manuscript Number : IJSRST185126
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Manju Rakesh, Patil N. M., " In Vitro Response of GA3 in Caulogenesis of Fiver nut", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.128-131, January-February-2018.
Journal URL : http://ijsrst.com/IJSRST185126

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