Effect of Lead Acetate Toxicity on Experimental Chick Embryo

Authors(2) :-Sawale Shweta, Kachhaway Archana R

Heavy metals are natural components of the Earth's crust and are important environmental pollutants which are mostly toxic even at very low concentrations. Lead is a common industrial and environment pollutant which is carcinogenic in animals. This study was conducted to understand the toxic effects of lead acetate on antioxidant enzymes and its bioaccumulation in liver and brain using a chick embryo as model. Chick embryos were divided into three groups, first group represented the healthy control and remaining two groups were injected as single dose of 20?l and 40?l/egg of lead acetate respectively, on 7th day of incubation with 1mg. concentration of lead acetate. On 11th and 14th day of incubation, control and treated chick embryos were sacrificed to collect liver and brain for biochemical assays. Different doses of lead acetate caused an increase or decrease of the activity of all investigated Enzymes, with a variable values, at almost all developmental stages. The lead that entered into the body is accumulated in organs and modifies the function of organ by changing the structure of cells of organs biochemical modifications in cells and functional variations of many of enzymes. Results of the present study clearly suggest that lead acetate induce toxic effects on the chick embryo.

Authors and Affiliations

Sawale Shweta
Department of Biotechnology, Savitribai Phule Pune University, Pune,Maharashtra, India
Kachhaway Archana R
Department of Zoology,Shri Sishivinayak Mahila Mahavidyalaya,Karve Nagar, Pune, Maharashtra, India

Lead Acetate, Antioxidant Enzymes, Liver, Brain

  1. A. Aebi H. (1984) :Catalase.Methods Enzymol., 105, 125-126.
  2. ATSDR- (2005): Draft toxicological profile for lead, US Department of health and human services, Atlanta,Georgia, USA. pp.102-225
  3. Clement, G.Y. and Paul, B.T (2007).:N-Acetyl-L-Cysteine Affords Protection against Lead-Induced Cytotoxicity and Oxidative Stress in Human Liver Carcinoma (HepG2) Cells. International Journal of Environmental Research andPublic Health, 4, 132-137.
  4. Cormier, J.M. and S.E. Bloom (1973):An in vivo study of the effects of X-radiation on the chromosomes of chick allantoic membranes, Mutation Res., 20, 77-85.Christie, N. T.; Costa, M. (1984).In vitro assessment of the toxicity of metal compounds. IV. Disposition of metals in cells: interaction with membranes, glutathione, metallothionein, and DNA. Biol.Trace Elem. Res. 6:139–158
  5. Denizeau F, Marion M. (1989).:Genotoxic effects of heavy metals in rat hepatocytes. Cell Bio Toxicol 5:15-25.
  6. Freeman BM, Vince MA (1974): Development of the avian embryo London: Chapman and Hall, 1-156.
  7. Garaza, A., R. Vega and E. Soto, (2006): Cellular mechanisms of lead neuro toxicity.Med. Sci. Monitor., 12(3): 57-65.
  8. Kirkman, H. N., Rolfo, M., Ferraris, A. M., Gaetani, G. F. (1999): Mechanisms of protection of catalase by NADPH.J. Biol. Chem. 274, 13908-139
  9. Marcia,C. Carvalho, Evelise M. Nazari, Marcelo Farina,1 and Yara M. R. Muller,(2008): Behavioral, Morphological, and Biochemical Changes after In Ova Exposure to Methylmercury in Chicks toxicological sciences 106(1), 180–185.
  10. Mudgal V, Madaan N, Mudgal A, Singh RB, Mishra S(2010): Effect of toxic metals on human health.Open Nutra J; 3: 94-99

Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 177-183
Manuscript Number : IJSRST185136
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Sawale Shweta, Kachhaway Archana R, " Effect of Lead Acetate Toxicity on Experimental Chick Embryo", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.177-183, January-February-2018.
Journal URL : http://ijsrst.com/IJSRST185136

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