Biochemical Characterization of Lipase from Fagopyrum Esculentum Seeds and Its Application

Authors

  • Bajoria Vani D.  Department of Chemistry, Division of Biochemistry, Savitribai Phule Pune University, Pune, Maharashtra, India
  • Sabharwal Sushma G.  Department of Chemistry, Division of Biochemistry, Savitribai Phule Pune University, Pune, Maharashtra, India

Keywords:

Lipase, enzyme immobilization, buckwheat

Abstract

Enzymes are biocatalysts, providing opportunity for economically sustainable industrial applications. Immobilization of enzyme further enhances its reusability. Lipase a hydrolytic enzyme, owing to its enantioselectivity and regioselectivity finds varied industrial applications. Lipases are used in detergent, food and various industries. In the present study, lipase was purified from the seeds of Fagopyrum esculentum (buckwheat). The enzyme was immobilized on chitosan beads. Optimization of different parameters viz. use of different cross linkers, concentration of enzyme, etc. for effective enzyme immobilization was carried out. Biochemical characterization of both the native and the immobilized enzyme was studied. Further, the reusability of the immobilized enzyme was also evaluated.

References

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International Journal of Scientific Research in Science and Technology

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Published

2018-01-20

Issue

Volume 5, Issue 1, January-February-2018

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Bajoria Vani D., Sabharwal Sushma G., " Biochemical Characterization of Lipase from Fagopyrum Esculentum Seeds and Its Application, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 5, Issue 1, pp.192-196, January-February-2018.