Biochemical Characterization of Lipase from Fagopyrum Esculentum Seeds and Its Application

Authors(2) :-Bajoria Vani D., Sabharwal Sushma G.

Enzymes are biocatalysts, providing opportunity for economically sustainable industrial applications. Immobilization of enzyme further enhances its reusability. Lipase a hydrolytic enzyme, owing to its enantioselectivity and regioselectivity finds varied industrial applications. Lipases are used in detergent, food and various industries. In the present study, lipase was purified from the seeds of Fagopyrum esculentum (buckwheat). The enzyme was immobilized on chitosan beads. Optimization of different parameters viz. use of different cross linkers, concentration of enzyme, etc. for effective enzyme immobilization was carried out. Biochemical characterization of both the native and the immobilized enzyme was studied. Further, the reusability of the immobilized enzyme was also evaluated.

Authors and Affiliations

Bajoria Vani D.
Department of Chemistry, Division of Biochemistry, Savitribai Phule Pune University, Pune, Maharashtra, India
Sabharwal Sushma G.
Department of Chemistry, Division of Biochemistry, Savitribai Phule Pune University, Pune, Maharashtra, India

Lipase, enzyme immobilization, buckwheat

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Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 192-196
Manuscript Number : IJSRST185138
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Bajoria Vani D., Sabharwal Sushma G., " Biochemical Characterization of Lipase from Fagopyrum Esculentum Seeds and Its Application", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.192-196, January-February-2018.
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