Biochemical Studies and Characterization of Immobilized Alkaline Phosphatases on Carboxyl-Functionalised Carbon Nanotubes

Authors(3) :-Vijaya D. Giramkar, Dr. Girish J. Phatak , Prof. Sushma G. Sabharwal

Alkaline phosphatase has been isolated from the seeds of 'Arachis hypoghaea' by ammonium sulphate fractionation (30-80%) and ion exchange chromatography. The isolated enzyme was studied for its biochemical properties viz. optimum pH and temperature. The purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) as well as a matrix of ethyl cellulose. The resulting two matrices were characterized by FTIR and SEM to obtain structural and morphological properties. By both methods the immobilised enzyme showed good activity comparable with the native enzyme. Reusability of the immobilised enzyme was demonstrated up to 8-10 cycles. The immobilized enzyme showed higher temperature stability. The investigation of cutting-edge nanomaterial with the help of enzyme immobilisation using exceptional techniques makes nanobiocatalyst of promising thirst for knowledge for biosensor applications.

Authors and Affiliations

Vijaya D. Giramkar
Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune, Maharashtra, India
Dr. Girish J. Phatak
Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune, Maharashtra, India
Prof. Sushma G. Sabharwal
Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University, Pune, Maharashtra, India

Alkaline phosphatase, Immobilization, CNTs, Biochemical studies

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Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 197-201
Manuscript Number : IJSRST185139
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Vijaya D. Giramkar, Dr. Girish J. Phatak ,Prof. Sushma G. Sabharwal, " Biochemical Studies and Characterization of Immobilized Alkaline Phosphatases on Carboxyl-Functionalised Carbon Nanotubes", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.197-201 , January-February-2018.
Journal URL : http://ijsrst.com/IJSRST185139

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